Buckyballs to fight pandemic: Water-soluble fullerene derivatives with pendant carboxylic groups emerge as a new family of promising SARS-CoV-2 inhibitors.

Herein, we present the first experimental study of individual water-soluble fullerene derivatives proving their ability to inhibit SARS-CoV-2 in vitro. The initial screening allowed us to identify a few new compounds that have demonstrated pronounced antiviral activity with IC values as low as 390 nM and selectivity indexes reaching 214. Time-of-addition analysis and molecular docking results suggested that the viral protease and/or the spike protein are the most probable targets inhibited by the fullerene derivatives.

Extraordinary SERS on nanostructured self-assembled silver films due to preliminary random nano-profiling of substrate and ultrasonic exposure of analyte during the deposition.

In this study, we have investigated the surface-enhanced Raman scattering (SERS) spectra of myoglobin on silver substrates with different morphology. The aim was to determine the optimal parameters of analyte and substrate preparation for obtaining of high-amplitude SERS spectra of proteins. It is shown that not only the morphology of the silver film, but also the method of analyte molecules deposition on the SERS substrate plays an important role.

Deep dive into the diversity and properties of rhodopsins in actinomycetes of the family Geodermatophilaceae.

In recent decades, most studies of microbial rhodopsins have focused on their identification and characterization in aquatic bacteria. In 2021, actinomycetes of the family Geodermatophilaceae, commonly inhabiting terrestrial ecosystems in hot and arid regions, have been reported to contain rhodopsins with DTEW, DTEF and NDQ amino acid motifs. An advanced bioinformatics analysis performed in this work additionally revealed NTQ rhodopsin and heliorhodopsins.

Effect of Macromolecular Architecture on Adhesion.

The behavior of single linear chains on a substrate is a well-studied area of polymer science. Herein, one of the most essential issues is the interaction of the chains with the substrate, which determines both macromolecular conformations near the substrate and adhesive properties of polymer materials. However, very little is known about the effect of macromolecular architecture on adhesion.

A randomized controlled trial on anonymizing reviewers to each other in peer review discussions.

Many peer-review processes involve reviewers submitting their independent reviews, followed by a discussion between the reviewers of each paper. A common question among policymakers is whether the reviewers of a paper should be anonymous to each other during the discussion. We shed light on this question by conducting a randomized controlled trial at the Conference on Uncertainty in Artificial Intelligence (UAI) 2022 conference where reviewer discussions were conducted over a typed forum.

A novel strain of Leishmania braziliensis harbors not a toti- but a bunyavirus.

Leishmania is a genus of the family Trypanosomatidae that unites obligatory parasitic flagellates causing a variety of vector-borne diseases collectively called leishmaniasis. The symptoms range from relatively innocuous skin lesions to complete failures of visceral organs. The disease is exacerbated if a parasite harbors Leishmania RNA viruses (LRVs) of the family Pseudototiviridae.

[Otohematoma as manifestation of auricular arteriovenous malformation].

Arteriovenous malformation (AVM, arteriovenous dysplasia) is one of the variants of congenital vascular defects formed as a result of a defect in the development of the arterial and venous systems during ontogenesis with the formation of direct messages between vessels of different diameters. In this regard, high-speed shunting of blood from the arterial part of the vascular system to the venous through fistulas of various calibers occurs. This disease is characterized by a variety of clinical manifestations.

[Clinical case of auricular gouty tophi].

A clinical case of a patient with tumors of the auricles on both sides is presented. With careful collection of anamnesis, it was found that the patient suffers from gout for a long time, has concomitant diseases that aggravate its course, and there is no compliance to treatment. Based on these data, the presence of gouty tophi, which are deposits of sodium monaurate in tissues in the form of nodes, is suspected.

[Methods of stenting the laryngeal-tracheal lumen after reconstructive surgical interventions. Literature review].

The article presents a literature review that analyzes methods of stenting the laryngeal-tracheal lumen after reconstructive surgical interventions. 49 literature sources were studied. The advantages and disadvantages of existing stents are determined.

[Topical issues of early diagnosis of hearing loss in newborn children].

A group of Russian specialists dealing with the problems of auditory function in premature babies touches upon important issues of early detection of hearing loss and deafness in this contingent of children born before the date of physiological birth. The purpose of the article was to argue the need for a personalized approach to the diagnosis of auditory function in premature and full-term babies depending on the timing of gestation and their somatic state at the time of birth, as well as the comprehensive rehabilitation of children with hearing loss and deafness. The article describes the advantages of the previously developed computer program Multiplicity of audiological monitoring in children of the first year of life with risk factors for hearing loss and deafness.

[Epidemiology of central auditory disorders in school-age children. Literature review].

Unlabelled: Central auditory disorders (CSD) - this is a violation of the processing of sound stimuli, including speech, above the cochlear nuclei of the brain stem, which is mainly manifested by difficulties in speech recognition, especially in noisy environments. Children with this pathology are more likely to have behavioral problems, impaired auditory, linguistic and cognitive development, and especially difficulties with learning at school.

Objective: To analyze the literature data on the epidemiology of central auditory disorders in school-age children.

[Differential diagnosis of acute maxillary ethmoiditis complicated by orbital abscess with dacryocystitis and phlegmon of the lacrimal sac in children].

Introduction: The differential diagnosis of orbital complications of rhinosinusitis with diseases of the lacrimal sac in childhood remains unresolved both due to the similarity of the symptoms of the diseases and due to certain diagnostic difficulties requiring computed tomography.

Objective: To develop an algorithm for routing a patient to the emergency department of a multidisciplinary emergency hospital based on clinical and diagnostic distinctive features of sinusitis with orbital complications and diseases of the lacrimal sac with reactive edema of the eyelids.

Materials And Methods: A retrospective cohort analysis of children's medical histories, who were treated in the otorhinolaryngological and ophthalmological departments of the Morozovskaya Children's City Clinical Hospital during 2022 was performed for orbital complications of rhinosinusitis or dacryocystitis with reactive edema of the eyelids.

[Efficacy and safety of topical antibiotic therapy in patients with acute external bacterial otitis: results of a retrospective study].

Otitis externa is one of the most common diseases in otorhinolaryngological practice frequently requiring prescription of analgesic medications and antimicrobials. The total of 2714 patients were included in the retrospective study to evaluate bacterial etiology, effectiveness, and safety of topical empirical treatment of patients with diagnosed otitis externa during 2018-2023. The most common pathogens isolated were (38.

[Acoustic reflex as one of the indicators of the maturity of the functioning of the auditory analyzer].

Unlabelled: The acoustic reflex is a physiological protective mechanism of the sound-conducting system of the auditory analyzer, which allows to identify various retrocochlear lesions of the human auditory system. The absence of its registration may indicate both pathology and immaturity of the auditory analyzer in children of the first year of life.

Objective: To register the acoustic reflex in children of the first year of life, to identify a full-fledged acoustic reflex at various frequencies and to analyze the dependence of its appearance on the timing of gestation.

[Immunomodulatory therapy in the postoperative period in chronic purulent otitis media].

Unlabelled: This paper presents the results of the evaluation of cellular and humoral immunity in chronic purulent otitis media (CPOM), as well as cytokine status, and studies the effect of azoximers bromide on the immunity system in CPOM.

Objective: To study the clinical and immunologic effectiveness of azoximer bromide in the postoperative period during tympanoplasty in CPOM patients.

Material And Methods: Forty-nine patients with mesotympanitis and epitympanitis were examined.

Inability of Ogataea parapolymorpha pho91-Δ mutant to produce active methanol oxidase can be compensated by inactivation of the PHO87 gene.

Cells of the methylotrophic yeast Ogataea parapolymorpha have two genes encoding low-affinity phosphate transporters: PHO87, encoding the plasma membrane transporter, and PHO91, encoding a protein, which is homologous to the Saccharomyces cerevisiae vacuolar membrane transporter. Earlier, we reported that inactivation of PHO91 in O. parapolymorpha interferes with methanol utilization due to the lack of activity of methanol oxidase encoded by the MOX gene.

Epidemiology and Molecular Characterisation of Multidrug-Resistant Isolated from Cow Milk.

Antimicrobial resistance (AMR) is a growing global concern and poses a significant threat to public health. The emergence of multidrug-resistant organisms, including , also presents a risk of transmission to humans through the food chain, including milk. This study aimed to investigate the prevalence of in raw milk in the Chattogram metropolitan area (CMA) of Bangladesh and their phenotypic and genotypic antimicrobial resistance patterns.

Gender Differences and Performance Changes in Sprinting and Long Jump Among Young Athletes.

This research aimed to examine the dynamics of changes in sprint and long jump performance and the age of gender divergence in well-trained young athletes specialising in athletics. Data were collected from 1249 male and 1497 female athletes aged 10 to 15 years who participated in the final round of the annual national athletics tournament "Shipovka Yunykh" from 2017 to 2019. The top 50 results in each age group for the 60-metre sprint and long jump were analysed.

The Protein Engineering of Zearalenone Hydrolase Results in a Shift in the pH Optimum of the Relative Activity of the Enzyme.

An acidic shift in the pH profile of zearalenone hydrolase (ZHD), the most effective and well-studied zearalenone-specific lactone hydrolase, is required to extend the range of applications for the enzyme as a decontamination agent in the feed and food production industries. Amino acid substitutions were engineered in the active center of the enzyme to decrease the pKa values of the catalytic residues E126 and H242. The T216K substitution provided a shift in the pH optimum by one unit to the acidic region, accompanied by a notable expansion in the pH profile under acidic conditions.

Toxins from Animal Venom-A Rich Source of Active Compounds with High Pharmacological Potential.

Animal venoms contain a huge variety of bioactive molecules-namely, toxins-with an almost combinatorial spectrum of biological activities [...

Influence of the Microbial Metabolite Acetyl Phosphate on Mitochondrial Functions Under Conditions of Exogenous Acetylation and Alkalization.

Background: Acetyl phosphate (AcP) is a microbial intermediate involved in the central bacterial metabolism. In bacteria, it also functions as a donor of acetyl and phosphoryl groups in the nonenzymatic protein acetylation and signal transduction. In host, AcP was detected as an intermediate of the pyruvate dehydrogenase complex, and its appearance in the blood was considered as an indication of mitochondrial breakdown.

The () Genetic Variant Is Associated with Muscle Fiber Size and Strength Athlete Status.

Background: Data on the genetic factors contributing to inter-individual variability in muscle fiber size are limited. Recent research has demonstrated that mice lacking the Arkadia (RNF111) N-terminal-like PKA signaling regulator 2N (; also known as ) gene exhibit reduced muscle fiber size, contraction force, and exercise capacity, along with defects in calcium handling within fast-twitch muscle fibers. However, the role of the gene in human muscle physiology, and particularly in athletic populations, remains poorly understood.

Nanopore Data-Driven T2T Genome Assemblies of Strains.

is a pathogenic fungus that infects flax and causes significant yield losses. In this study, we assembled the genomes of four highly virulent strains using the Oxford Nanopore Technologies (ONT, R10.4.

Boldenone and Testosterone Production from Phytosterol via One-Pot Cascade Biotransformations.

Testosterone (TS) and its 1(2)-dehydrogenated derivative boldenone (BD) are widely used in medicine, veterinary science and as precursors in organic synthesis of many therapeutic steroids. Green production of these compounds is possible from androstenedione (AD) enzymatically, or from phytosterol (PS) using fermentation stages. In this study, the ascomycete sp.

Evaluation of Color Difference Models for Wide Color Gamut and High Dynamic Range.

Color difference models (CDMs) are essential for accurate color reproduction in image processing. While CDMs aim to reflect perceived color differences (CDs) from psychophysical data, they remain largely untested in wide color gamut (WCG) and high dynamic range (HDR) contexts, which are underrepresented in current datasets. This gap highlights the need to validate CDMs across WCG and HDR.