Ultrasonic Effect on the Growth of Crystals from Aqueous Electrolyte Solutions on Polymer Substrates: The Role of Isotopic Composition of Liquid.

The peculiarities of the crystal formation from supersaturated aqueous solutions of CuSO on polymer substrates were studied using X-ray diffractometry. During the crystal formation, the test solutions were irradiated with one or two counter-propagating ultrasonic beams. Test solutions were prepared using natural deionized water with a deuterium content of 157 ± 1 ppm.

Characterization and Evaluation of Zero-Order Release System Comprising Glycero-(9,10-trioxolane)-trialeate and PLA: Opportunity for Packaging and Biomedicine Applications.

Glycerol-(9,10-trioxolane) trioleate (OTOA) is a promising material that combines good plasticizing properties for PLA with profound antimicrobial activity, which makes it suitable for application in state-of-the-art biomedical and packaging materials with added functionality. On the other hand, application of OTOA in PLA-based antibacterial materials is hindered by a lack of knowledge on kinetics of the OTOA release. In this work, the release of glycero-(9,10-trioxolane) trioleate (OTOA) from PLA films with 50% OTOA content was studied during incubation in normal saline solution, and for the first time, the kinetics of OTOA release from PLA film was evaluated.

Effect of Side Substituent on Comb-like Polysiloxane Membrane Pervaporation Properties During Recovery of Alcohols C2-C4 from Water.

The pervaporation properties of membranes based on comb-like polysiloxanes when C-C alcohols are removed from water were studied for the first time. It was established that membranes based on comb-like polysiloxanes with linear aliphatic and organosilicon substituents have increased permeability selectivity for C alcohols. The obtained results were interpreted from the point of view of the solubility of the components of the separated mixture in polysiloxanes.

Relaxation Phenomena in Low-Density and High-Density Polyethylene.

A study was conducted on the internal friction spectra and temperature dependencies of the frequency of free damped oscillatory processes excited in the investigated samples of low-density polyethylene (LDPE) and high-density polyethylene (HDPE) over a temperature range from -150 °C to +150 °C. It was found that the internal friction spectra exhibit several local dissipative processes of varying intensity, which manifest in different temperature intervals. The structure of the internal friction spectra and the peaks of dissipative losses are complex, as evidenced by the occurrence of sharp, locally temperature-dependent jumps in the intensity of dissipative losses observed throughout the entire temperature range.

Characterization and Rheological Properties of Ultra-High Molecular Weight Polyethylenes.

The molecular characteristics and rheological properties of three UHMWPE samples were investigated. The high-temperature GPC method was used for characterizing UHMWPE samples used. The interpretation of the measurement results was based on calibration using the PS standard and the approximation of the PS data by linear and cubic polynomials, as well as on the data for linear PE.

High-Performance Porous Supports Based on Hydroxyl-Terminated Polysulfone and CO/CO-Selective Composite Membranes.

The scope of this work was to develop a thin-film composite (TFC) membrane for the separation of CO/CO mixtures, which are relevant for many processes of gas processing and gasification of carbon-based feedstock. Special attention was given to the development of highly permeable porous polysulfone (PSF) supports (more than 26,000 GPU for CO) since both the selective and support layers contribute significantly to the overall performance of the TFC membrane. The PSF porous support is widely used in commercial and lab-scale TFC membranes, and its porous structure and other exploitation parameters are set during the non-solvent-induced phase separation (NIPS) process.

Virus-like Particles Produced in Plants: A Promising Platform for Recombinant Vaccine Development.

The capsid proteins of many viruses are capable of spontaneous self-assembly into virus-like particles (VLPs), which do not contain the viral genome and are therefore not infectious. VLPs are structurally similar to their parent viruses and are therefore effectively recognized by the immune system and can induce strong humoral and cellular immune responses. The structural features of VLPs make them an attractive platform for the development of potential vaccines and diagnostic tools.

On the Cryptic Speciation in the Mosses with East Asia-East North America Disjunction: A Case Study of Two Poorly Understood Mosses from the Southern Extremity of the Russian Far East.

A survey of the moss flora of the southernmost part of the Russian Primorsky Territory yielded several intriguing taxa, whose identity is assessed herein based on an integrative morpho-molecular approach. was previously known in inland Asia only from the Sino-Himalayan region and the new locality is distant from the earlier known ones to ca. 3000 km.

Effect of Selenium, Copper and Manganese Nanocomposites in Arabinogalactan Matrix on Potato Colonization by Phytopathogens and .

The effect of chemically synthesized nanocomposites (NCs) of selenium (Se/AG NC), copper oxide (Cu/AG NC) and manganese hydroxide (Mn/AG NC), based on the natural polymer arabinogalactan (AG), on the processes of growth, development and colonization of potato plants in vitro was studied upon infection with the causative agent of potato blackleg-the Gram-negative bacterium -and the causative agent of ring rot-the Gram-positive bacterium (). It was shown that the infection of potatoes with reduced the root formation of plants and the concentration of pigments in leaf tissues. The treatment of plants with Cu/AG NC before infection with stimulated leaf formation and increased the concentration of pigments in them.

Identification and Analysis of , , , and Gene Families in .

(β-ketoacyl-acyl carrier protein (ACP) synthases II), (fatty acid thioesterases), (stearoyl-ACP desaturase), and (fatty acid desaturases) are the vital gene families involved in fatty acid (FA) synthesis in L. However, information on the number and location of these genes and which ones are key to the formation of FAs in fruit seeds and pulp was not complete. Our study aimed to solve this issue using the available genomic sequences and transcriptome data that we obtained.

Nanopore Data-Driven Chromosome-Level Assembly of Flax Genome.

Flax is an important crop grown for seed and fiber. Flax chromosome number is 2n = 30, and its genome size is about 450-480 Mb. To date, the genomes of several flax varieties have been sequenced and assembled.

Differential Immunological Responses of Adult Domestic and Bighorn Sheep to Inoculation with Type Strain Y98.

Bighorn sheep (BHS) populations have been reported to experience high levels of morbidity and mortality following infection with . This contrasts with the subclinical presentation in domestic sheep (DS). Understanding this difference requires baseline knowledge of pre- and post-infection immune responses of both species.

Genomic and Phenotypic Characterization of sp. nov., Amicetin-Producing Actinobacteria Isolated from Bamboo Rhizospheric Soil.

In our large-scale search for antimicrobial-producing bacteria, we isolated an actinomycete strain from rhizospheric soil of . The strain designated BP-8 showed noticeable antibacterial activity. BP-8 was subjected to a whole-genome analysis via a polyphasic taxonomy approach, and its antibacterial metabolite was identified by HRLS-MS.

Complete Genome Sequence of a Novel Strain TA Isolated from Western Siberia Chernevaya Taiga Soil.

A whole genome sequence of a new strain of the nitrogen-fixing bacterium known for its diverse plant growth-promoting bacteria (PGPB), was obtained for the first time. The strain, designated e AT, was isolated during a soil analysis in the Chernevaya taiga of Western Siberia, a unique and fertile forest ecosystem known for its diverse plant growth-promoting bacteria (PGPB). The genome under study is fully assembled into seven circular molecules, none of which are unequivocally plasmids, with a total length of 6.

Lateral Flow Immunosensing of Typhimurium Cells in Milk: Comparing Three Sequences of Interactions.

To ensure the safety of foodstuffs, widespread non-laboratory monitoring for pathogenic contaminants is in demand. A suitable technique for this purpose is lateral flow immunoassay (LFIA) which combines simplicity, rapidity, and productivity with specific immune detection. This study considered three developed formats of LFIA for Typhimurium, a priority pathogenic contaminant of milk.

Sulfate-Reducing Bacteria Isolated from an Oil Field in Kazakhstan and a Description of sp. nov.

Sulfidogenic bacteria cause numerous issues in the oil industry since they produce sulfide, corroding steel equipment, reducing oil quality, and worsening the environmental conditions in oil fields. The purpose of this work was to isolate and taxonomically identify the sulfidogenic bacteria responsible for the corrosion of steel equipment at the Karazhanbas oil field (Kazakhstan). In this study, we characterized five sulfidogenic strains of the genera , , and isolated from the formation water of the Karazhanbas oil field (Kazakhstan).

Biooxidation of a Pyrite-Arsenopyrite Concentrate Under Stressful Conditions.

Gold recovery from refractory pyrite-arsenopyrite concentrates using stirred tank reactor biooxidation is widely applied worldwide. Therefore, studies to address the characteristic problem of this technology are urgent. The goal of the present work was to research the possibility of counteracting the negative effects of unfavorable conditions (increasing pulp density and temperature) on the biooxidation of pyrite-arsenopyrite concentrate in laboratory-scale stirred tank reactors using additional carbon supply in the form of CO.

Highly Targeted Detection of Priority Phytopathogen : From Obtaining Polyclonal Antibodies to Development and Approbation of Enzyme-Linked Immunoassay and Lateral Flow Immunoassay.

is a bacterial phytopathogen that causes soft and black rot and actively spreads worldwide. Our study is the first development of immunoassays for detecting . We immunized rabbits and obtained serum with an extremely high titer (1:10).

In Vitro Dynamic Model Evaluation of Meropenem Alone and in Combination with Avibactam Against Carbapenemase-Producing .

A potential strategy to maintain the efficacy of carbapenems against carbapenemase-producing (CPKP) is their combination with carbapenemase inhibitors. To address these issues, the effectiveness of a novel combination of meropenem with avibactam against CPKP was studied. Additionally, the applicability of a pharmacokinetically-based approach to antibiotic/inhibitor minimum inhibitory concentration (MIC) determinations to better predict efficacy was examined.

p53: The Multifaceted Roles of Covalent Modifications in Cancer.

The p53 protein has attracted huge research interest over several decades due to its role as one of the most important tumor suppressors in mammals, which orchestrates a synchronous response from normal cells in the body to various forms of stress. The diverse cellular activities of the p53 protein are regulated mainly via its post-translational modifications (PTMs). PTMs affect p53 on several levels: at the level of the assembly of tetrameric complexes on DNA to transactivate its target genes, at the level of the assembly of tetrameric complexes on DNA to transactivate its target genes; at the level of proteolysis in the absence of stress; and on the contrary, at the level of augmented protein stability in response to stress signals.

N-Glycoside of Indolo[2,3-]pyrrolo[3,4-]carbazole LCS1269 Exerts Anti-Glioblastoma Effects by G2 Cell Cycle Arrest and CDK1 Activity Modulation: Molecular Docking Studies, Biological Investigations, and ADMET Prediction.

Indolo[2,3-]pyrrolo[3,4-]carbazole scaffold is successfully used as an efficient structural motif for the design and development of different antitumor agents. In this study, we investigated the anti-glioblastoma therapeutic potential of glycosylated indolocarbazole analog LCS1269 utilizing in vitro, in vivo, and in silico approaches. Cell viability was estimated by an MTT assay.

Effects of the Tobacco Defensin NaD1 Against Susceptible and Resistant Strains of .

Today, is still the most common cause of both local and life-threatening systemic candidiasis. The spread of resistant fungal strains has resulted in an urgent need to search for new promising antimycotics. Here, we investigated the antifungal action of the tobacco defensin NaD1 against susceptible and resistant to azoles and echinocandins strains of .

RNA Stability: A Review of the Role of Structural Features and Environmental Conditions.

The stability of RNA is a critical factor in determining its functionality and degradation in the cell. In recent years, it has been shown that the stability of RNA depends on a complex interaction of external and internal factors. External conditions, such as temperature fluctuations, the level of acidity of the environment, the presence of various substances and ions, as well as the effects of oxidative stress, can change the structure of RNA and affect its stability.

A Novel Pot-Economy Approach to the Synthesis of Triantennary GalNAc-Oligonucleotide.

N-Acetylgalactosamine (GalNAc) is an efficient and multifunctional delivery tool in the development and synthesis of chemically modified oligonucleotide therapeutics (conjugates). Such therapeutics demonstrate improved potency in vivo due to the selective and efficient delivery to hepatocytes in the liver via receptor-mediated endocytosis, which is what drives the high interest in this molecule. The ways to synthesize such structures are relatively new and have not been optimized in terms of the yields and stages both in lab and large-scale synthesis.

In Vitro Study of Antiviral Properties of Compounds Based on 1,4-Dioxane Derivative of Closo-Decaborate Anion with Amino Acid Ester Residues Against Influenza Virus A/IIV-Orenburg/83/2012(H1N1)pdm09.

New derivatives of the -decaborate anion [BH-O(CH)O(CH)C(O)-L-OCH] (An) (: L = Trp; : L = His; : L = Met; : L = Ala(2-oxopyrrolidin-3-yl) (Pld) were synthesized and isolated as tetraphenylphosphonium salts (PhP)An. Anions ; ; , and contain a pendant functional group from the L-tryptophan methyl ester, L-histidine methyl ester, L-methionine methyl ester, or methyl 2-amino-3-(2-oxopyrrolidin-3-yl)propanoate (-Trp-OCH, -His-OCH, -Met-OCH, or -Pld-OCH) residue, respectively, bonded with the boron cluster anion through the oxybis[(ethane-2,1-diyl)oxy] spacer. This pacer is formed as a result of the nucleophilic opening of the attached dioxane molecule in the [BHO(CH)O] starting derivative.