Using Zebrafish Models to Study Epitranscriptomic Regulation of CNS Functions.

Epitranscriptomic regulation of cell functions involves multiple post-transcriptional chemical modifications of coding and non-coding RNA that are increasingly recognized in studying human brain disorders. Although rodent models are presently widely used in neuroepitranscriptomic research, the zebrafish (Danio rerio) has emerged as a useful and promising alternative model species. Mounting evidence supports the importance of RNA modifications in zebrafish CNS function, providing additional insights into epitranscriptomic mechanisms underlying a wide range of brain disorders.

Advantages of aptamers as ligands upon protein detection by AFM-based fishing.

A combined AFM/MS method was employed for protein registration in solution. This method is based on reversible specific capturing of a target protein from a large volume of analyzed solution onto a small sensor area of a chip with immobilized aptamer ligands. Fishing of the core antigen of hepatitis C virus (HCVcoreAg) from 10 M solution of this protein in buffer was carried out.

Unlocking Mechanisms for Soil Organic Matter Accumulation: Carbon Use Efficiency and Microbial Necromass as the Keys.

Soil microorganisms transform plant-derived C (carbon) into particulate organic C (POC) and mineral-associated C (MAOC) pools. While microbial carbon use efficiency (CUE) is widely recognized in current biogeochemical models as a key predictor of soil organic carbon (SOC) storage, large-scale empirical evidence is limited. In this study, we proposed and experimentally tested two predictors of POC and MAOC pool formation: microbial necromass (using amino sugars as a proxy) and CUE (by O-HO approach).

B-doped nano-hydroxyapatite facilitates proliferation and differentiation of osteoblasts.

Purpose: We aimed to explore the mechanism by which Boron-doped nano-hydroxyapatite (B-nHAp) facilitates the proliferation and differentiation of osteoblasts through controlled release of B.

Methods: B-nHAp characterization was accomplished by means of X-ray diffraction, scanning electron microscopy, inductively coupled plasma mass spectrometry, and transmission electron microscopy. Human bone marrow mesenchymal stem cells (hBMSCs) were subjected to flow cytometry, alizarin red S staining, and cell counting kit-8 assay for proliferation and differentiation determination.

Morpho-physiological digestive features of genetically closely related sympatric lacustrine whitefish forms with different feeding habits.

The effect of different feeding habits on gut morphology and digestive function has been intensively studied during the last decades but sympatric closely related fishes are relatively rare objects of such studies. In the present study, we have identified both morphological and physiological changes in the digestive system of a sympatric pair of whitefish represented by "normal" Coregonus lavaretus pidschian (benthivorous) and "dwarf" C. l.

Frequency-specific changes in prefrontal activity associated with maladaptive belief updating in volatile environments in euthymic bipolar disorder.

Bipolar disorder (BD) involves altered reward processing and decision-making, with inconsistencies across studies. Here, we integrated hierarchical Bayesian modelling with magnetoencephalography (MEG) to characterise maladaptive belief updating in this condition. First, we determined if previously reported increased learning rates in BD stem from a heightened expectation of environmental changes.

Redesigning methionine γ-lyase for improved stability and catalytic activity in the β-elimination reaction for the synthesis of thiosulfinates.

Pyridoxal 5'-phosphate (PLP)-dependent enzymes are involved in many cellular processes and possess unequalled catalytic versatility. Rational design through site-directed mutagenesis is a powerful strategy for creating tailor-made enzymes for a wide range of biocatalytic applications. PLP-dependent methionine γ-lyase (MGL), which degrades sulfur-containing amino acids, is an encouraging enzyme for many therapeutic purposes - from combating bacterial resistant strains and fungi to antitumor activity.

Outcomes after medical treatment for primary aldosteronism: an international consensus and analysis of treatment response in an international cohort.

Background: Primary aldosteronism can be treated medically but there is no standardised method to evaluate treatment outcomes. We aimed to develop criteria for assessing the outcomes of targeted medical treatment of primary aldosteronism, analyse outcomes across an international cohort, and identify factors associated with a complete treatment response.

Methods: An international panel of 31 primary aldosteronism experts used the Delphi method to reach consensus on the definition of complete, partial, or absent biochemical and clinical outcomes of medical treatment of primary aldosteronism.

SMAD2/3 signaling determines the colony architecture in a hydrozoan, Dynamena pumila.

Most hydrozoan cnidarians form complex colonies that vary in size, shape, and branching patterns. However, little is known about the molecular genetic mechanisms responsible for the diversity of the hydrozoan body plans. The Nodal signaling pathway has previously been shown to be essential for setting up a new body axis in a budding Hydra.

Designing and clinical application of a 3D-printed personalized model of a radiofrequency needle guide with a maxillary fixator for puncture of the gasserian ganglion for trigeminal neuralgia treatment.

Objective: The purpose of this study was to present a newly designed 3D-printed personalized model (3D PPM) of a radiofrequency needle guide with a maxillary fixation for gasserian ganglion (GG) puncture.

Methods: Implementation of 3D CT-guided radiofrequency therapy of the GG with and without use of 3D PPM was analyzed. The following parameters were assessed: radiation time, dose area product, air kerma reference point, pain severity during the puncture needle insertion, prosopalgia regression degree (according to visual analog scale) and the severity of facial numbness (according to the Barrow Neurological Institute scale) in the early postoperative period, and postpuncture complications.

Novel Allele HLA-B*52:130, Identified by Next-Generation Sequencing.

The new HLA-B*52:130 allele showed one nonsynonymous nucleotide difference compared to the HLA-B*52:01:01:01 allele in codon 170.

A case of simultaneous bilateral pulmonary resection for arteriovenous pulmonary malformation.

Pulmonary arteriovenous malformation (PAVM) is a congenital vascular pathology, which is caused by the presence of a direct connection between the branches of the artery and the veins of the lungs, and the discharge of unoxygenated blood into the arterial bed. Arteriovenous malformations are characterized by a wide variety of clinical manifestations and, in some cases, may be accompanied with severe circulatory disorders.

Targeting CD45 by gene-edited CAR T cells for leukemia eradication and hematopoietic stem cell transplantation preconditioning.

Hematopoietic stem cell transplantation (HSCT) is widely used to treat patients with life-threatening hematologic and immune system disorders. Current nontargeted chemo-/radiotherapy conditioning regimens cause tissue injury and induce an array of immediate and delayed adverse effects, limiting the application of this life-saving treatment. The growing demand to replace canonical conditioning regimens has led to the development of alternative approaches, such as antibody-drug conjugates, naked antibodies, and CAR T cells.

Revision of the millipede family Dalodesmidae in Madagascar, with descriptions of two new Malagasy species of Cook, 1896 (Diplopoda, Polydesmida).

The family Dalodesmidae Cook, 1896 in the fauna of Madagascar is reviewed and shown to presently encompass eight species in three genera: Cook, 1896 (six species), Brölemann, 1916 (one species), and de Saussure & Zehntner, 1897 (one species). These genera are diagnosed, and their respective species keyed, all being endemic to Madagascar proper and/or the immediately adjacent islets of Nosy Be and/or Nosy Sakatia. currently contains six species, including two new, all supplied either with brief descriptive notes and available iconography or extensive descriptions and new illustrations, as follows: (Brandt, 1841), from an unspecified locality in Madagascar, now redescribed from a male specimen from Makira, northeastern Madagascar; (Attems, 1898), from Nosy Be Isle; Hoffman, 1974, from Ambohimitombo, central Madagascar; Cook, 1896 (= (de Saussure & Zehntner, 1897), ), originally described from an unspecified locality in central Madagascar, with a male type and additional males identified as recorded from the Andasibe National Park (= Périnet) in east-central Madagascar.

Fabrication of high-density vertical CNT arrays using thin porous alumina template for biosensing applications.

This paper explores the process of forming arrays of vertically oriented carbon nanotubes (CNTs) localized on metal electrodes using thin porous anodic alumina (PAA) on a solid substrate. On a silicon substrate, a titanium film served as the electrode layer, and an aluminium film served as the base layer in the initial film structure. A PAA template was formed from the Al film using two-step electrochemical anodizing.

Novel efficient reservoir computing methodologies for regular and irregular time series classification.

Time series is a data structure prevalent in a wide range of fields such as healthcare, finance and meteorology. It goes without saying that analyzing time series data holds the key to gaining insight into our day-to-day observations. Among the vast spectrum of time series analysis, time series classification offers the unique opportunity to classify the sequences into their respective categories for the sake of automated detection.

Healthy aging changes in conventional frequency bands of neuroelectric brain activity reconstructed from resting-state MEG.

Age-related dependencies of electric and spectral powers in conventional frequency bands were studied by the newly proposed method of detailed spectral analysis. The magnetic encephalograms (MEG) and magnetic resonance images (MRI) of the head were obtained from the open archive Cam-CAN. The spatial distributions of elementary spectral components (MEG-based functional tomograms) were reconstructed from MEG for 501 subjects (248 males and 253 females, ages 18-88 years, mean age 54.

Expanding the Variety of Pyridinium-Based bis-QACs with Antimicrobial Properties: Investigation into Linker Structure-Activity Correlation.

For decades quaternary ammonium compounds (QACs) have served as main component of a top antiseptic and disinfectant compositions. Among them, bis-QACs are the most prominent and effective class of biocides. Although mono-QACs still dominate the antiseptic market, their activity against Gram-negative bacteria is largely inferior to bis-QACs.

Zinc, copper, copper-to-zinc ratio, and other biometals in blood serum and tumor tissue of patients with colorectal cancer.

The objective of the present study was to assess serum and cancerous tissue biometal levels in colorectal cancer (CRC) patients, and its relation to disease severity. A total of 90 CRC patients and 97 controls were involved in the present study. The level of biometals in blood serum and colon tissues (only in CRC cases) was evaluated by inductively-coupled plasma mass-spectrometry.

Comparison of management options for spontaneous bleeding into soft tissues in patients with COVID-19.

Spontaneous bleeding into soft tissues was a common complication of anticoagulant therapy among critically ill patients during the COVID-19 pandemic. This study sought to evaluate the impact of different treatment methods on survival among COVID-19 patients with active or self-resolved spontaneous bleeding into soft tissues. Methods used to treat CT-confirmed spontaneous hematomas in COVID-19 patients included nonoperative management (NOM), angiography (AG) with transarterial embolization (TAE) and open surgery (OS).

Polariton lattices as binarized neuromorphic networks.

We introduce a novel neuromorphic network architecture based on a lattice of exciton-polariton condensates, intricately interconnected and energized through nonresonant optical pumping. The network employs a binary framework, where each neuron, facilitated by the spatial coherence of pairwise coupled condensates, performs binary operations. This coherence, emerging from the ballistic propagation of polaritons, ensures efficient, network-wide communication.

Composition, Structure, and Thermal Properties of Potassium Acetate Hydrates.

The crystal structure determines the properties of compounds and materials, although one can find simple yet industrially relevant compounds such as potassium acetate (KOAc) and its hydrates for which the properties and even the composition still remain misunderstood, owing to the lack of structural data. In this study, the crystal structures of KOAc polymorphs and hydrates were determined for the first time. The water content in the crystal hydrates was reliably determined revealing two new phases 3KOAc·2HO and KOAc·HO ( = 0.

Left ventricular elastance with resting volumetric transthoracic echocardiography identifies different phenotypes in heart failure with preserved ejection fraction: A retrospective analysis of a multicenter prospective observational study.

Background: Heart failure with preserved ejection fraction (HFpEF) is a heterogeneous entity including patients with different phenotypes of near normal, normal, and supernormal left ventricular (LV) function.

Objectives: To assess the value of resting LV elastance (also known as force) with transthoracic echocardiography (TTE) to identify HFpEF phenotypes.

Methods: In a prospective, observational, multicenter study, 2380 HFpEF patients were recruited from July 2016 to May 2024.

Preclinical evaluation of Ga-labeled folic acid conjugates for visualization of inflammatory foci.

Introduction: Folate receptors (FR) have been considered a convenient target for different radiopharmaceuticals in recent years. Multifarious Ga-labeled folate conjugates have been proposed as promising agents for the PET imaging of FR-overexpressing malignant neoplasms. In addition, radiolabeled folate-based conjugates can be effective for imaging non-tumor pathological foci characterized by a pronounced cluster of activated macrophages.

Whisker-free lithium electrodeposition by tuning electrode microstructure.

Growth of lithium whiskers or dendrites is the major obstacle towards safe and stable utilization of lithium metal anodes in rechargeable batteries. In this study, we look deeper into the mechanism of lithium electrodeposition. We find that before lithium whisker or dendrite nucleation occurs, lithium is deposited into the grain boundaries of the metal electrode, which we directly observed in the focused ion beam cross-sections of the lithium electrode.