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Productive Infection of Human Embryonic Stem Cell-Derived NKX2.1+ Respiratory Progenitors with Human Rhinovirus.

Stem Cells Transl Med

June 2015

Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Murdoch Childrens Research Institute, Parkville, Victoria, Australia; Telethon Kids Institute, Centre for Health Research, School of Paediatrics and Child Health, Centre for Health Research, and Centre for Cell Therapy and Regenerative Medicine, School of Medicine and Pharmacology, University of Western Australia, Nedlands, Western Australia, Australia; Department of Respiratory Medicine, Princess Margaret Hospital for Children, Perth, Western Australia, Australia; Richie Centre, Monash Prince Henry's Medical Research Institute, Clayton, Victoria, Australia

Unlabelled: Airway epithelial cells generated from pluripotent stem cells (PSCs) represent a resource for research into a variety of human respiratory conditions, including those resulting from infection with common human pathogens. Using an NKX2.1-GFP reporter human embryonic stem cell line, we developed a serum-free protocol for the generation of NKX2.

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