Development of a high-throughput platform for quantitation of histone modifications on a new QTOF instrument.

Histone post-translational modifications (PTMs) regulate gene expression patterns through epigenetic mechanisms. The 5 histone proteins (H1, H2A, H2B, H3, and H4) are extensively modified, with over 75 distinct modification types spanning more than 200 sites. Despite strong advances in mass spectrometry-based approaches, identification and quantification of modified histone peptides remains challenging due to factors such as isobaric peptides, pseudo-isobaric PTMs, and low stoichiometry of certain marks.

Advancing mitochondrial therapeutics: Synthesis and pharmacological evaluation of pyrazole-based inhibitors targeting the mitochondrial pyruvate carrier.

Inhibition of mitochondrial pyruvate transport via the mitochondrial pyruvate carrier (MPC) has shown beneficial effects in treating metabolic diseases, certain cancers, various forms of neurodegeneration, and hair loss. These benefits arise either from the direct inhibition of mitochondrial pyruvate metabolism or from the metabolic rewiring when pyruvate entry is inhibited. However, current MPC inhibitors are either nonspecific or possess poor pharmacokinetic properties.

Neonatal Seizures and Associated Neurobehavioral Profiles in Preschool Age Children.

Background: Neonatal seizures are common with acute brain injury. Up to 25% of survivors develop postneonatal epilepsy. We hypothesized postneonatal epilepsy diagnosed by age 24 months would increase risk for early markers of neurobehavioral disorders than acute provoked neonatal seizures alone.

Escaping the ordinary: a review of escape rooms in medical and veterinary education.

Background: Escape rooms (ERs), immersive role-playing games that require participants to solve a series of puzzles within a set time to achieve a specific goal, have gained popularity as innovative educational tools.

Methods: A systematic review was conducted using the PRISMA guidelines, a comprehensive search of PubMed, Cochrane, Web of Science, and Scopus, for articles published between inception of journals to April 2024, focusing on the integration, outcomes, and participants' perceptions of ERs in medical and veterinary education.

Results: A total of 619 articles were retrieved, of which 12 articles met the inclusion criteria for final analysis.

Vagus nerve stimulation in treatment-resistant depression: A one-year, randomized, sham-controlled trial.

Background: Few treatments are available for individuals with marked treatment-resistant depression (TRD).

Objective: Evaluate the safety and effectiveness of FDA-approved adjunctive vagus nerve stimulation (VNS) in patients with marked TRD.

Methods: This 12-month, multicenter, double-blind, sham-controlled trial included 493 adults with marked treatment-resistant major depression who were randomized to active or no-stimulation sham VNS for 12 months.

PI3K signaling and lysyl oxidase is critical to corneal stroma fibrosis following mustard gas injury.

Sulfur mustard gas (SM), an alkylating and vesicating agent, has been used frequently in many wars and conflicts. SM exposure to the eye results in several corneal abnormalities including scar/fibrosis formation. However, molecular mechanism for SM induced corneal fibrosis development is poorly understood.

Day-level associations among alcohol use, simultaneous alcohol and cannabis use, and both regretted and positive romantic/sexual experiences.

Background: Young adults who drink heavily, including those who simultaneously use cannabis, experience both positive and regretted romantic/sexual experiences. Both gender and past sexual assault severity may also play a role in the valence of romantic/sexual experiences. The current study focuses on day-level correlates of romantic/sexual experiences, assessing whether: (1) number of drinks or simultaneous alcohol and cannabis use (vs.

Challenges and Supports for Implementing Health Equity During National Accreditation Among Small Local Health Departments in the United States.

Objective: This article focuses on supports and challenges to health equity that small local health departments (LHDs) experienced while working toward national reaccreditation or Pathways Recognition with the Public Health Accreditation Board's Standards & Measures Version 2022 (PHAB S&M v2022).

Design: The study team conducted 22 qualitative interview sessions with members of health department leadership teams.

Setting: In the spring of 2024, participants from 4 small LHDs in the western and midwestern regions of the United States participated in individual remote interview sessions.

Leveraging SERS and Transformer Models for Simultaneous Detection of Multiple Pesticides in Fresh Produce.

The widespread use of pesticides in agriculture poses food safety and environmental risks, highlighting the need for rapid detection techniques to mitigate contamination. Surface-enhanced Raman spectroscopy (SERS) coupled with machine learning provides a powerful approach for the detection and quantification of multiple pesticides in agricultural products. This study introduces the SERSFormer-2.

The Effect and Acceptability of an HIV Education Intervention for Adolescents With Intellectual Disability in Uganda: A Quasi-Experimental Study.

Adolescents with intellectual disability (ID) are often deficient in knowledge about HIV/AIDS. This pilot study evaluated the impact and acceptability of an HIV education intervention for adolescents with mild-to-moderate ID delivered in a peri-urban setting in Uganda. This quasi-experimental study involved 60 adolescents with mild to moderate ID evenly split between the intervention and control groups.

An Access-Focused Patient-Centric Value Assessment Framework for Medication Formulary Decision-Making in Immune-Mediated Inflammatory Diseases.

The healthcare system in the United States (US) is complex and often fragmented across national and regional health plans which exhibit substantial variability in benefit design and formulary policies for accessing medications. We propose an access-focused value assessment framework for formulary decision-making for medications to manage immune-mediated inflammatory diseases (IMIDs), where patients are at the center of this framework. Formulary decision-making for IMID medications can be a challenging, even daunting, task with continuously evolving and enhanced treat-to-target goals.

A single-center learning curve for stereotactic laser amygdalohippocampotomy and a surgical framework to manage failures.

Objective: Stereotactic laser amygdalohippocampotomy (SLAH) is a minimally invasive procedure for mesial temporal lobe epilepsy that preserves more tissue than open procedures. As a result, although patients have better functional outcomes, more patients do not achieve seizure freedom. The rate at which this occurs is evolving with improved surgical practices.

Spinal TNF-α receptor 1 is differentially required for phrenic long-term facilitation (pLTF) over the course of motor neuron death in adult rats.

Introduction: Intrapleural injections of cholera toxin B conjugated to saporin (CTB-SAP) result in selective respiratory (, phrenic) motor neuron death and mimics aspects of motor neuron disease [(, amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA)], such as breathing deficits. This rodent model allows us to study the impact motor neuron death has on the output of surviving phrenic motor neurons as well as the compensatory mechanisms that are recruited. Microglial density in the phrenic motor nucleus as well as cervical gene expression of markers associated with inflammation (.

Sex Disparities Affecting Postoperative Outcomes After Total Elbow Arthroplasty.

Purpose: To investigate sex disparities in 30-day postoperative outcomes of total elbow arthroplasty.

Methods: The American College of Surgeons-National Surgical Quality Improvement Program files were queried for all patients who underwent a total elbow arthroplasty (TEA) between 2006 and 2020. Sex disparities in preoperative variables were studied using Fisher exact tests.

Development and validation of animal variant classification guidelines to objectively evaluate genetic variant pathogenicity in domestic animals.

Assessing the pathogenicity of a disease-associated genetic variant in animals accurately is vital, both on a population and individual scale. At the population level, breeding decisions based on invalid DNA tests can lead to the incorrect inclusion or exclusion of animals and compromise the long-term health of a population, and at the level of the individual animal, lead to incorrect treatment and even life-ending decisions. Criteria to determine pathogenicity are not standardized, i.

A Retrospective Analysis of Mortality Due to Pneumonia and Renal Disease in a Midwestern Patient Population.

Background: The impact of pneumonia (PNA) with concomitant renal disease (RD) has not been fully investigated in a United States Midwestern patient population, despite the morbidity and mortality associated with such diseases.

Materials And Methods: A retrospective cohort study was performed on International Classification of Diseases, 10 Revision (ICD-10) data from a hospital system located in Southwest Missouri. Data was acquired from patients admitted between January 2019 and December 2021.

A safe insect-based chikungunya fever vaccine affords rapid and durable protection in cynomolgus macaques.

Eilat (EILV)/chikungunya virus (CHIKV), an insect-based chimeric alphavirus was previously reported to protect mice months after a single dose vaccination. The underlying mechanisms of host protection are not clearly defined. Here, we assessed the capacity of EILV/CHIKV to induce quick and durable protection in cynomolgus macaques.

Relationship Between Adverse Childhood Experiences and Resilience: The Indirect Effect of a Sense of Purpose in Life.

Purpose: This study examined the association between adverse childhood experiences (ACEs) and resilience through a sense of purpose in life (SP) among 578 college students, applying resilience theory and positive psychology.

Materials And Methods: Using a cross-sectional, online survey design, data were collected. Most respondents were female (71.

"The PO-Driven Model": A Basic Science Pipeline for the Bioeconomy with Solutions Inspired by Convergent Evolution for Connecting Parallel Research Ideas.

Basic science research, also called "curiosity-driven research," is fundamental work done with no immediate economic goals but rather a focus on discovery for discovery's sake. However, basic science research is often needed to seed more applied, economically-oriented, research. Both basic and applied research efforts are important aspects of the "bioeconomy" defined here as the contributions to the overall economy from various biology-related fields spanning everything from museum-based natural history research to agricultural food and material production to healthcare.

Inadequate Intensification of LDL-cholesterol lowering therapy after coronary revascularization: Insights from the GOULD registry.

Background: Patients with a history of coronary revascularization are at a higher risk for subsequent cardiovascular events and all-cause mortality. Lowering LDL-cholesterol (LDL-C) levels post-revascularization significantly reduces these risks.

Methods: This analysis compared LDL-C-lowering therapies at baseline and over time among patients with and without prior coronary revascularization in the GOULD registry (a prospective multicenter cohort study).

Self-reported Hearing Quality of Life for Adolescent Cochlear Implant Recipients: A Longitudinal Study.

Background:  Clinicians are increasingly interested in self-reported hearing-specific quality of life (HQoL) for cochlear implant (CI) recipients, including pediatric CI recipients.

Purpose:  (1) To compare HQoL of adolescent CI recipients to those of peers with typical hearing (TH); (2) to examine, longitudinally, HQoL for a set of CI recipients; and (3) to determine the effects of child, demographic, audiological, speech perception, and language variables on adolescent HQoL.

Research Design:  Hearing Environments and Reflections on Quality of Life (HEARQL) questionnaires were completed by children with CIs at elementary (HEARQL-26) and adolescent (HEARQL-28) ages.

Observations on the Etiology of Involutional Entropion.

Purpose: To review evidence supporting proposed anatomic etiologies of involutional entropion and propose additional potential contributing factors.

Methods: A literature review was performed to identify publications describing possible etiologies of involutional entropion. The author's clinical observations and information supporting new proposed causes are presented.

Quantifying the global biodiversity of Proterozoic eukaryotes.

The global diversity of Proterozoic eukaryote fossils is poorly quantified despite its fundamental importance to the understanding of macroevolutionary patterns and dynamics on the early Earth. Here we report a new construction of fossil eukaryote diversity from the Paleoproterozoic to early Cambrian based on a comprehensive data compilation and quantitative analyses. The resulting taxonomic richness curve verifies Cryogenian glaciations as a major divide that separates the "Boring Billion" and Ediacaran periods, with the former characterized by a prolonged stasis, and the latter by greater diversity, more-rapid turnover, and multiple radiations and extinctions.

Implementation of the RQI System: Baseline Skills and Self-Report Competence and Confidence Data From 12 NLN Inaugural Change Agent Nursing Programs.

Aim: This article describes the implementation, baseline cardiopulmonary resuscitation (CPR) skills, and competence and confidence in skills of participants in 12 nursing programs piloting the Resuscitation Quality Improvement (RQI) program. Of 1,847 participants, 175 had not previously completed a CPR course.

Method: Schools could choose the sequence for completing baselines skills and required e-learning modules.