Type IX Secretion System Effectors and Virulence of the Model Flavobacterium columnare Strain MS-FC-4.

Flavobacterium columnare causes columnaris disease in wild and cultured freshwater fish and is a major problem for sustainable aquaculture worldwide. The F. columnare type IX secretion system (T9SS) secretes many proteins and is required for virulence.

Comparison of Accelerated Resolution Therapy for PTSD Between Veterans With and Without Prior PTSD Treatment.

Introduction: Post-traumatic stress disorder (PTSD) is a psychiatric disorder commonly caused by a traumatic event(s) and prevalent among service members and veterans. Accelerated Resolution Therapy (ART) is an emerging "mind-body" psychotherapy for PTSD that is generally briefer and less expensive than current first-line treatments, such as cognitive processing therapy (CPT) and prolonged exposure (PE) therapy. This study examined the results of ART for treatment of military-related PTSD, with stratification by prior PTSD treatment types, including service members/veterans with reported residual PTSD symptoms following receipt of first-line recommended psychotherapy.

Perchlorate exposure does not induce obesity or non-alcoholic fatty liver disease in zebrafish.

Perchlorate is a water-soluble contaminant found throughout the United States and many other countries. Perchlorate competitively inhibits iodide uptake at the sodium/iodide symporter, reducing thyroid hormone synthesis, which can lead to hypothyroidism and metabolic syndromes. Chronic perchlorate exposure induces hepatic steatosis and non-alcoholic fatty liver disease (NAFLD) in developing threespine stickleback (Gasterosteus aculeatus).

DESCRIPTION AND PHYLOGENETIC AFFINITIES OF A NEW SPECIES OF NEOPSILOTREMA (DIGENEA: PSILOSTOMIDAE) FROM LESSER SCAUP, AYTHYA AFFINIS (ANSERIFORMES: ANATIDAE).

Neopsilotrema is a small genus of psilostomid digeneans parasitic in the intestine of birds in the Palearctic and Nearctic. At present, the genus includes 4 species: Neopsilotrema lisitsynae from the Palearctic and Neopsilotrema affine, Neopsilotrema lakotae, and Neopsilotrema marilae from the Nearctic. Herein, we describe a new species, Neopsilotrema itascae n.

The Monoheme Subunit of Respiratory Alternative Complex III Is Not Essential for Electron Transfer to Cytochrome in Flavobacterium johnsoniae.

Bacterial alternative complex III (ACIII) catalyzes menaquinol (MKH) oxidation, presumably fulfilling the role of cytochromes / in organisms that lack these enzymes. The molecular mechanism of ACIII is unknown and so far the complex has remained inaccessible for genetic modifications. The recently solved cryo-electron microscopy (cryo-EM) structures of ACIII from Flavobacterium johnsoniae, Rhodothermus marinus, and Roseiflexus castenholzii revealed no structural similarity to / and there were variations in the heme-containing subunits ActA and ActE.

A Generalized Equation for Prediction of VO2peak from a Step Test.

This study aimed to develop an equation to reduce variability of VOpeak prediction from a step test and compare VOpeak prediction from the new equation to the Queen's College Step Test (QCST). The development group (n=86; 21.7±2 years) was utilized to develop the SDState step test equation to predict relative VOpeak.

Bowel function and care: Guidelines for the care of people with spina bifida.

Purpose: Bowel dysfunction, such as constipation and fecal incontinence, has a significant impact on health, activities of daily living, and quality of life among people with spina bifida. Secondary complications may result from bowel dysfunction and include urologic dysfunction, loss of skin integrity, shunt (hydrocephalus) function, as well as loss of social opportunities and employability.

Methods: Using a consensus building methodology, the guidelines for management of bowel dysfunction in spina bifida were written by experts in the field of spina bifida and bowel function and care.

Integument: Guidelines for the care of people with spina bifida.

Purpose: Skin-related issues have a significant impact on health, activities of daily living, and quality of life among people with spina bifida. Data presented by select clinics that participate in the National Spina Bifida Patient Registry reported that 26% of individuals had a history of pressure injuries with 19% having had one in the past year. The spina bifida community lack direct guidelines on prevention of these and other skin related issues.

Multifunctional bilateral muscle control of vocal output in the songbird syrinx.

Songbirds produce complex vocalizations by coordinating neuromuscular control of syrinx, respiratory system, and upper vocal tract. The functional roles of syringeal muscles have been documented mainly with correlative data, which have suggested that synergistic activation plays a role in the fine control of vocal features. However, the specific involvement of individual muscles in achieving this fine control is still largely unknown.

Reported Sexual-offense Incidents in the United States: Arrest Disparities between Women and Men.

This study examined the effect of an offender's sex (male/female) on whether sexual-offense incidents reported to law enforcement culminated in an arrest. Two hypotheses, chivalry and evil woman, are relied upon and suggest that the probability of arrest differs for women and men, yet in differing directions. The chivalry hypothesis suggests women are treated more leniently than men and, therefore, less likely to be arrested.

Transforming learning: Applying community and systems level interventions through a poster project.

Background: Public health nursing practice is population-focused with a goal of improving health outcomes. Nurse educators are challenged to identify teaching-learning strategies to transform students' perspectives and knowledge of public health concepts.

Purpose: The purpose of the poster project was to evaluate students' ability to meet Quad Council Coalition Competency Review Task Force competencies for public health nursing.

Climate and phylogenetic history structure morphological and architectural trait variation among fine-root orders.

Fine roots mediate below-ground resource acquisition, yet understanding of how fine-root functional traits vary along environmental gradients, within branching orders and across phylogenetic scales remains limited. Morphological and architectural fine-root traits were measured on individual root orders of 20 oak species (genus Quercus) from divergent climates of origin that were harvested after three growing seasons in a glasshouse. These were then compared with similar measurements obtained from a phylogenetically diverse dataset of woody species from the Fine-Root Ecology Database (FRED).

Effect of a rapid e-learning module and brief interprofessional simulation event on medical and nursing student collaborative attitudes and behaviors.

Objectives Undergraduate nursing and first-year medical students participated in a brief, scenario-based, interprofessional event. The experimental group only was provided an innovative, rapid e-learning instructional module focused on interprofessional roles/responsibilities and communication prior to the event. Methods Pre-post attitudes toward physician-nurse collaboration were surveyed, and collaborative behaviors were observed during the event.

Examining jurors' ability to meet the constitutional requirement of narrowing in capital sentencing.

The US Supreme Court has required that death penalty schemes narrow the class of persons eligible for a death sentence. Through the selection requirement, juries must use mitigating and aggravating evidence jointly to select the offenders engaged in the worst of the worst crimes. This study utilized between-subjects experimental design to test juror's ability to narrow directly.

Q-gases in a late-forming refractory interplanetary dust particle: A link to comet Wild 2.

We report the structure, chemical composition, O, Al-Mg, He, and Ne isotope systematics of an interplanetary dust particle, "Manchanito". These analyses indicate that Manchanito solidified as refractory glass (with oxidized Fe but reduced Ti) in a chondrule-like formation environment more than 3.2 Myr after CAIs, after which it was exposed to Q-like noble gases in the dissipating solar nebula.

Women's work at end of life: The intersecting gendered vulnerabilities of patients and caregivers in rural Malawi.

Gender inequality in the form of gender-based violence manifests throughout the course of women's lives but has a particularly unique impact at end of life. We sampled 26 patients and 14 caregivers for this qualitative critical ethnographic study. The study purpose was to describe the lived experience of female palliative care patients in rural Malawi and their caregivers.

The effects of surface albedo and initial lignin concentration on photodegradation of two varieties of Sorghum bicolor litter.

Decomposition of plant litter exposed to solar radiation appears to be a significant contributor to carbon cycling in some ecosystems. One factor that may influence incident solar radiation exposure on litter is surface albedo. Snow and soils with high reflectivity may enhance photodecomposition, especially in litter that stands upright for extended periods.

The Carboxy-Terminal Region of Flavobacterium johnsoniae SprB Facilitates Its Secretion by the Type IX Secretion System and Propulsion by the Gliding Motility Machinery.

SprB moves rapidly along the cell surface, resulting in gliding motility. SprB secretion requires the type IX secretion system (T9SS). Proteins secreted by the T9SS typically have conserved C-terminal domains (CTDs) belonging to the type A CTD or type B CTD family.

Scientific methodology of the development of the Guidelines for the Care of People with Spina Bifida: An initiative of the Spina Bifida Association.

Background: We combined literature review and consensus-building methodologies to develop health care guidelines for people with Spina Bifida across the life span.

Objective: The present paper describes the methodology used to update and expand this fourth edition of the Guidelines for the Care of People with Spina Bifida ("Guidelines"). This process was a fundamental initiative within the Spina Bifida Collaborative Care Network.

Using a Decision Tree to Guide Bowel Management in Spina Bifida.

Background: When born with spina bifida, there are numerous neurologic disorders that accompany this birth defect, including neurogenic bowel. Proactive, systematic, and rational approaches can lead to continence and a more functional lifestyle [1].

Methods: Based on the evidence in the literature and expert experience, our approach to bowel management was developed as a step by step, individualized approach.

Associations between Biotic Integrity and Sport Fish Populations in Upper Midwest, USA Rivers, with Emphasis on Smallmouth Bass.

Indices of biotic integrity (IBIs) are used to assess ecosystem health of streams and rivers. Streams and rivers with high IBI scores should support abundant and healthy populations of recreationally important sport fishes. However, the fundamental assumption that IBI scores and sport fish populations are associated needs to be examined.

Nurses' Perceptions of Basic Palliative Care in the Hmong Population.

The purpose of this study was to describe basic palliative care from the acute care nurses' perspective with consideration for culturally diverse populations. Participants focused their responses based on their experiences with Hmong patients. The concept of cultural safety was explored to improve basic palliative care practice in acute care settings.

Deafness and loss of cochlear hair cells in the absence of thyroid hormone transporters Slc16a2 (Mct8) and Slc16a10 (Mct10).

Transmembrane proteins that mediate the cellular uptake or efflux of thyroid hormone potentially provide a key level of control over neurodevelopment. In humans, defects in one such protein, solute carrier SLC16A2 (MCT8) are associated with psychomotor retardation. Other proteins that transport the active form of thyroid hormone triiodothyronine (T3) or its precursor thyroxine (T4) have been identified in vitro but the wider significance of such transporters in vivo is unclear.

Public Health and School Nurses' Perceptions of Barriers to HPV Vaccination in Missouri.

Human Papilloma Virus (HPV) is associated with various forms of cancer and is preventable with vaccination. Nurses are in a unique position to directly influence a person's decision to receive the HPV vaccine. A statewide sample of both school and public health nurses was used to assess knowledge and attitudes related to the HPV vaccine as well as nurse's behaviors and behavioral intentions toward educating parents and recommending the vaccine.

Physicochemical characteristics of pristine and functionalized graphene.

Graphene-based nanomaterials have received significant attention in the last decade due to their interesting properties. Its electrical and thermal conductivity and strength make graphene well suited for a variety of applications, particularly for use as a composite material in plastics. Furthermore, much work is taking place to utilize graphene as a biomaterial for uses such as drug delivery and tissue regeneration scaffolds.