Methylation of foreign DNA overcomes the restriction barrier of and allows efficient genetic manipulation.

Unlabelled: causes bacterial cold-water disease (BCWD) in salmonids and other fish, resulting in substantial economic losses in aquaculture worldwide. The mechanisms uses to cause disease are poorly understood. Despite considerable effort, most strains of have resisted attempts at genetic manipulation.

Nurses on the outside, problems on the inside! The duty of nurses to support unions.

Healthcare is increasingly impacted by chronic short staffing of nurses, which causes and is caused by increased nurse burnout and decreased retention. Nurses' unions seek to address these problems by proposing safer nurse-to-patient ratios, retention bonuses for working through the COVID-19 pandemic, Personal Protective Equipment (PPE) stockpiles, sabbatical leaves, measures aimed at reducing workplace violence, and maintaining or increasing wages and benefits to keep nurses at the bedside. Chronic short staffing and burnout directly affect the quality and availability of patient care-as the International Council of Nurses has pointed out, there is no healthcare without healthcare workers.

Real-time intervention to increase daily chlorhexidine bathing and reduce central line-associated bloodstream infections.

Background: After an increase of central line-associated bloodstream infections (CLABSIs) at our community hospital in 2021, a case-control study suggested that patients with CLABSIs were 3.0 times more likely to have missed daily chlorhexidine gluconate (CHG) bathing than patients without CLABSIs.

Objective: To increase the rate of daily CHG bathing in hospitalized patients with central lines and subsequently reduce the number of CLABSIs.

Death Anxiety: An Exploration of Professional Counselor Experiences.

This quantitative research study guided by Terror Management Theory (TMT) provides a theoretical framework to understand death anxiety among practicing counselors while providing support for implementing death education into counseling graduate programs. The total sample comprised 97 total practicing counselors and current counseling students who were recruited from online platforms consisting of professional memberships, listservs, private practice, and Facebook groups. This study explored the level of death anxiety in counselors (measured by the Death Anxiety Scale-Extended (DAS-E; Templer et al.

Note similarities affect syntactic stability in zebra finches.

The acquisition of an acoustic template is a fundamental component of vocal imitation learning, which is used to refine innate vocalizations and develop a species-specific song. In the absence of a model, birds fail to develop species typical songs. In zebra finches (Taeniopygia guttata), tutored birds produce songs with a stereotyped sequence of distinct acoustic elements, or notes, which form the song motif.

Evaluation of vascular aging on measures of cardiac function and mechanical efficiency: insights from modeling.

Introduction: This study evaluated the hypothesis that vascular aging (VA) reduces ventricular contractile function and mechanical efficiency (ME) using the left ventricular pressure-volume (PV) construct.

Methods: A previously published computational model (CM) was modified to evaluate the hypothesis in two phases. In phase I, the CM included five settings of aortic compliance () from normal to stiff, studied at a heart rate of 80 bpm, and phase II included the normal to stiff settings evaluated at 60, 100, and 140 bpm.

Evaluation of a Disaster Preparedness Curriculum and Medical Students' Views on Preparedness Education Requirements for Health Professionals.

Objective: In general, medical students perceive themselves as inadequately prepared to assist in disasters. This study evaluated the impact of a disaster preparedness curriculum and medical students' views toward required preparedness education for health care professionals.

Methods: A comprehensive disaster preparedness curriculum was evaluated on its effect on medical students' views on preparedness education requirements, preparedness, and prior disaster training using self-report survey methodology.

Effects of caudal fin size on tail-flip jump performance.

Fishes are generally considered to be fully aquatic, but some voluntarily strand themselves on land to escape poor water conditions, predators, or to exploit terrestrial niches. The tail-flip jump is a method of terrestrial locomotion performed by small fishes without apparent morphological specialization, but few studies have investigated the role the caudal fin has on the tail-flip jump. We hypothesized that fish with larger caudal fins would perform shorter individual tail-flip jumps and not be able to sustain jumping in extended terrestrial excursions.

Age-Related Microaggressions: Their Frequency, Emotional Impact, and Relationship to Negative Affect.

Age-related microaggressions are forms of ageist discrimination that occur in day-to-day interactions. This study aimed to validate the findings of a previous study, to identify common age-related microaggressions, and determine how affect influences emotional reactions to microaggressions. Using an online survey, participants ( = 200) were asked about their experience with age-related microaggressions, their affect, and their physical health.

Differential gene expression and developmental pathologies associated with persistent organic pollutants in sentinel fish in Troutman Lake, Sivuqaq, Alaska.

Persistent organic pollutants (POPs) are lipophilic compounds that bioaccumulate in animals and biomagnify within food webs. Many POPs are endocrine disrupting compounds that impact vertebrate development. POPs accumulate in the Arctic via global distillation and thereby impact high trophic level vertebrates as well as people who live a subsistence lifestyle.

Integrative analyses reveal outcome-associated and targetable molecular partnerships between TP53, BRD4, TNFRSF10B, and CDKN1A in diffuse large B-cell lymphoma.

Diffuse large B-cell lymphoma (DLBCL) is a common, genomically heterogenous disease that presents a clinical challenge despite the success of frontline regimens and second-line chimeric antigen receptor T-cell (CAR-T) therapy. Recently, genomic alterations and tumor microenvironment features associated with poor CAR-T response have been identified, namely those to the TP53 tumor suppressor gene. This retrospective analysis aimed to integrate various data to identify genomic partnerships capable of providing further clarity and actionable treatment targets within this population.

Implementation and efficacy of knowledge translation frameworks in family focused nursing care: A scoping review.

Aim: To provide an overview of the characteristics, variety and outcomes of knowledge translation (KT) strategies used in nursing care involving adult patients and their family members.

Background: The gap in providing family nursing practice could be due to a lack of explicit KT frameworks and understanding of ways to translate evidence-based knowledge into clinical practice.

Design: A scoping review conducted according to the Joanna Briggs Institute.

International Family Nursing Association: toolkit of resources for caring for refugee/migrating families.

Objective: To report the experience of the International Family Nursing Association (IFNA) Practice Committee on developing a Toolkit of resources to care for refugee/migrating families as a response to the global migration and refugee crisis.

Method: Qualitative and descriptive study, experience report, which describes the development of a toolkit of resources for caring for refugee/migrating families.

Results: The development of this Toolkit of resources to care for refugee/migrating families is supported by current literature related to family-centered evaluation and intervention, culturally sensitive practice based on family strengths; statements of positioning on immigrant and refugee families; and nursing and health organizations that addressed the health of the refugee family.

Drought response strategies are coupled with leaf habit in 35 evergreen and deciduous oak (Quercus) species across a climatic gradient in the Americas.

Distinct survival strategies can result from trade-offs in plant function under contrasting environments. Investment in drought resistance mechanisms can enhance survivorship but result in conservative growth. We tested the hypothesis that the widespread oaks (Quercus spp.

Family Interviews Inform School-based Nursing for Children with Type 1 Diabetes and their Families.

The purpose of this study was to better understand the burden(s) associated with type 1 diabetes mellitus (T1DM) on school-aged youth and families and subsequently identify strategies school nurses can adopt to reduce the impact of this disease. Family interviews (n = 5 families, comprised of 15 individual participants) were conducted using a semi-structured interview guide to further explore family members' experiences with T1DM. Directed content analysis was employed for theme identification.

Creating a quadruple aim model for nursing education.

Baccalaureate nursing education is moving to adopt the new American Association of Colleges of Nursing Essentials for Professional Nursing Education. As identified in two of the six domains of the essentials, graduates need to be prepared to address population health and utilize informatics and healthcare technologies. Community/public health nursing also has eight domains for generalist nurses linked to population health which will help prepare a skilled nursing workforce for the 21st century.

Age-Related Microaggressions: A Descriptive Study.

Background And Objectives: The aim of this study was to expand the literature on ageism by exploring the phenomenon of age-related microaggressions.

Research Design And Methods: Fifty-one participants completed an online survey asking a series of questions about their experiences with microaggressions related to their age. Responses were summarized using a thematic analysis.

Rotaxane nanomachines in future molecular electronics.

As the electronics industry is integrating more and more new molecules to utilize them in logic circuits and memories to achieve ultra-high efficiency and device density, many organic structures emerged as promising candidates either in conjunction with or as an alternative to conventional semiconducting materials such as but not limited to silicon. Owing to rotaxane's mechanically interlocked molecular structure consisting of a dumbbell-shaped molecule threaded through a macrocycle, they could be excellent nanomachines in molecular switches and memory applications. As a nanomachine, the macrocycle of rotaxane can move reversibly between two stations along its axis under external stimuli, resulting in two stable molecular configurations known as "ON" and "OFF" states of the controllable switch with distinct resistance.

Countercontrol: A Relational Frame Theory (RFT) Account and Revival of a 70-Year-Old Skinnerian Term.

Countercontrol is a Skinnerian operant concept that posits that an individual's attempts to exert control over another person's behavior may evoke a countercontrolling response from the person being controlled that functions to avoid or escape the potentially aversive conditions generated by the controller. Despite Skinner's historical concerns regarding the detrimental effects of countercontrol in terms of hindering optimal societal growth and cultural evolution, the concept has not been widely applied within behavior analysis. Drawing from recent developments in rule-governed behavior and relational frame theory, this article seeks to explicate countercontrol from a contemporary behavior analytic perspective and presents several modern-day societal applications.

Examining the Provision of School-Based Athletics in Public High Schools Among Girls in the United States.

Background: In order to better understand some of the barriers to athletic opportunities for girls, we examined school-level characteristics to assess how they are associated with both the provision and participation in athletic opportunities for girls across US public high schools.

Methods: Cross-sectional data for this study comes from the bi-annual Civil Rights Data Collection which collects school-level information for all US public schools between the 2009-2010 through 2017-2018 school years.

Results: Several important school-level characteristics were found to limit the provision of athletic opportunities, participation rates and athletic participation gender equity ratios for girls and included the percent of the student body who were eligible for free lunch and who were racial minorities.

A holistic health determinants model for public health nursing education and practice.

Baccalaureate nursing graduates (BSNs) in the 21st century need to be prepared to manage the population health needs of diverse populations across the lifespan and take actions to improve health equity. A need for a Holistic Health Determinants Model that included the Social Determinants of Health was identified. A model incorporating the Healthy People 2020 Health Determinants Model and the Healthy People 2030 Social Determinants of Health Model was developed.