[Decreased incidence of peripheral nuclear staining pattern by indirect immunofluorescent antibody technique using HEp-2 cells].

The immunofluorescence technique using HEp-2 cells is widely used for the detection of antinuclear antibodies in sera of patients with connective tissue diseases. Among different patterns of nuclear staining the incidence of peripheral pattern which is mainly produced by anti double-stranded DNA antibodies has been markedly decreased after using HEp-2 cells as nuclear substrates. Thirty-five sera in interphase cells and 44 sera in metaphase cells showed peripheral pattern at 1:1 serum dilution from 70 patients with various connective tissue diseases including 46 systemic lupus erythematosus.

[What are IgG antibodies to single-stranded DNA measured by enzyme immunoassay--re-evaluation of their clinical significance].

Enzyme immunoassay (EIA) to detect IgG antibodies to single-stranded DNA (ssDNA EIA) and EIA to detect anti double-stranded DNA antibodies (dsDNA EIA) have been developed and widely used in Japan. However, antigenecity of ssDNA and independence of anti ssDNA antibodies, and their clinical significance are still obscure. IgG anti dsDNA and ssDNA antibodies were simultaneously measured by dsDNA EIA and ssDNA EIA in sera of two hundred and eighty five patients from systemic lupus erythematosus (SLE) including other connective tissue diseases.

[Detection of anti double-stranded DNA antibodies by RECOMBIGEN anti DNA kit].

Anti double-stranded DNA antibodies were detected in patients with various connective tissue diseases by using RECOMBIGEN Anti DNA kit (Japan DPC Co), newly developed utilizing highly purified double-stranded DNA obtained from E. coli plasmid by recombinant technique. High levels of anti double-stranded DNA antibodies were mostly found and distributed in patients with systemic lupus erythematosus and the allied disorders.