3 results match your criteria: "Meharry Medical Collegegrid.259870.1[Affiliation]"
HIV-1 Preintegration Complex Preferentially Integrates the Viral DNA into Nucleosomes Containing Trimethylated Histone 3-Lysine 36 Modification and Flanking Linker DNA.
J Virol
September 2022
The Center for AIDS Health Disparities Research, Meharry Medical Collegegrid.259870.1, Nashville, Tennessee, USA.
HIV-1 DNA is preferentially integrated into chromosomal hot spots by the preintegration complex (PIC). To understand the mechanism, we measured the DNA integration activity of PICs-extracted from infected cells-and intasomes, biochemically assembled PIC substructures using a number of relevant target substrates. We observed that PIC-mediated integration into human chromatin is preferred compared to genomic DNA.
View Article and Find Full Text PDFTrypanosoma brucei Tim50 Possesses PAP Activity and Plays a Critical Role in Cell Cycle Regulation and Parasite Infectivity.
mBio
October 2021
Department of Microbiology, Immunology, and Physiology, School of Medicine, Meharry Medical Collegegrid.259870.1, Nashville, Tennessee, USA.
Trypanosoma brucei, the infective agent for African trypanosomiasis, possesses a homologue of the translocase of the mitochondrial inner membrane 50 (TbTim50). It has a pair of characteristic phosphatase signature motifs, DXDX(T/V). Here, we demonstrated that, besides its protein phosphatase activity, the recombinant TbTim50 binds and hydrolyzes phosphatidic acid in a concentration-dependent manner.
View Article and Find Full Text PDFHuman Three Prime Repair Exonuclease 1 Promotes HIV-1 Integration by Preferentially Degrading Unprocessed Viral DNA.
J Virol
August 2021
Center for AIDS Health Disparities Research, Meharry Medical Collegegrid.259870.1, Nashville, Tennessee, USA.
Article Synopsis
- TREX1 is a key enzyme in mammalian cells that helps degrade HIV-1 DNA, playing a role in the virus's ability to escape the immune system, but its exact function during early HIV-1 infection is unclear.
- The study found that HIV-1 infection leads to an increase in TREX1 levels and its movement into the nucleus, yet this overexpression actually enhances the integration of viral DNA rather than hindering it.
- TREX1 actively stimulates the integration process of HIV-1 by preserving the integration-competent viral DNA and promoting the function of preintegration complexes, highlighting how the virus manipulates cellular mechanisms to its advantage.