[The results of increasing the thickness of soft tissues after using collagen matrices and connective tissue grafts].

The Study Aims: To evaluate and compare the growth of gingiva around dental implants following the use of collagen matrices and connective tissue grafts (CTG).

Materials And Methods: The study included 80 study participants, who were divided into four groups based on the type of material used to enhance gingival thickness. Two groups utilized collagen matrices, Fibro-Gide and FibroMatrix as materials, while the other two groups utilized CTG obtained from the hard palate or tubercle area of the maxilla as controls.

[Assessment of the risk of hearing loss in children with cystic fibrosis].

Background: Cystic fibrosis (CF) is a severe hereditary disease with a multisystem lesion. Manifestations of CF include severe infectious purulent lesions of all parts of the respiratory tract, including purulent rhinosinusitis with nasal polyps. The involvement of the sinonasal region and the need for systemic use of ototoxic drugs (primarily aminoglycosides to treat resistant bacterial infection) potentially create a risk of both conductive and sensorineural hearing loss (SNHL).

[Features of course of pain syndrome in patients with schwannomatosis].

Objective: To identify the characteristics of pain syndrome in patients with schwannomas depending on genetic predisposition.

Material And Methods: The study included 46 patients with peripheral, spinal and intracranial schwannomas, corresponding to the schwannomatosis phenotype according to the 2022 clinical criteria. All patients underwent sequencing of the , and and a copy number study in the .

[Study of properties of three-dimensional matrices manufactured by antisolvent 3D printing for reconstruction of extensive bone defects in dentistry and maxillofacial surgery].

Objective: The aim of the study to study of physical, mechanical and biocompatible properties of the matrices manufactured by antisolvent 3D printing from the solutions of polylactide-co-glycolide in tetraglycol.

Material And Methods: Three-dimensional scaffolds were made from a solution of polylactide-co-glycolide mixed with tetraglycol using antisolvent 3D printing. The elastic properties and the structure of the obtained matrices were studied.

[Relative survival and cell adhesion of multipotent mesenchymal cells from primary teeth pulp on the surface membranes and sponges produced from collagen].

Objective: To study the behavior of SHED cell culture on different types of materials for the regeneration of periodontal tissues with different porosity.

Material And Methods: Porous collagen material Fibro-Gide (Geitstlich Pharma AG, Switzerland), designed to increase the volume of the gum and the barrier collagen membrane Bio-Gide (Geitstlich Pharma AG, Switzerland) were studied on SHED cultures. As a control sample, a Spongostan sponge made of gelatin (Johnson & Johnson Medical, UK) with the most pronounced porosity and wettability was used.

[OTOF-related auditory neuropathy spectrum disorder].

Unlabelled: Otoferlin () gene mutations are the most common cause of hereditary ANSD according to investigations in several countries.

The Aim: Of this study was to estimate the prevalence of mutations in Russian children with ANSD and evaluate audiological and clinical features of -related ANSD.

Patients And Methods: 28 children with bilateral ANSDwere enrolled in the investigation.

[Hearing loss due to mutations or lack of the gene coding protein stereocillin].

Objective: The description of a clinical picture and audiological features at the hearing loss caused by changes of a gene, coding protein stereocillin (MIM: 606440). Mutations in the numerous genes responsible for the inner ear proteins are the reason for congenital sensorineural hearing loss. The main cause of congenital bilateral sensorineural hearing loss in the Russian Federation are mutations in gene it reaches up 68% of cases identified in infancy.

Peripheral blood serum from healthy donors contains antibodies against the fragment of transcribed region of ribosomal repeat.

Enzyme immunoassay showed that blood serum from healthy donors contains specific high-affinity antibodies (apparent association constant > or = 5 x 10(9) M(-1)) against a fragment of transcribed region of ribosomal DNA repeat of human serum, which are present in a free form or are bound to extracellular DNA. Preheating of the serum at 55 degrees C and high ionic strength (1.5 M NaCl) had no effect on the interaction of antibodies with this fragment.

Blood serum DNA in patients with rheumatoid arthritis is considerably enriched with fragments of ribosomal repeats containing immunostimulatory CpG-motifs.

We previously hypothesized that the sequence of transcribed region of human ribosomal repeats is selectively accumulated in circulating extracellular DNA due to its increased resistance to double-strand breaks caused by accumulation of single-chain breaks produced by nucleases. The contents of rDNA in blood serum DNA and in DNA from leukocytic nuclei both in healthy donors and in patients with rheumatoid arthritis were compared using dot hybridization method. By the content of non-methylated CpG-repeats, transcribed region of rDNA is identical to bacterial DNA, which is characterized by potent immunostimulatory effect.

Melphalan in ultralow doses decreases the severity of experimental colitis in mice.

We studied the effect of melphalan in ultralow doses on mice with experimental colitis induced by substitution of drinking water for 5% dextran sulfate. Daily treatment with melphalan in a dose of 0.025 mg/kg improved the general state of animals.

Stimulatory effect of fragments from transcribed region of ribosomal repeat on human peripheral blood lymphocytes.

Fragments from the transcribed region of the ribosomal repeat include considerable amounts of unmethylated CpG DNA motifs. These motifs activate immune cells via the interaction with Toll receptors. In vitro experiments confirmed the stimulatory effect of transcribed region of ribosomal repeat on human lymphocytes.

Markers of general pathology.

Studies of the protein composition of leukocytes revealed markers of general pathology: 2 protein parameters (53K/H2A>0.25; 43K/H2A<1.61) differed from normal in 1627 patients with various pathologies.

[The accumulation of single-stranded breaks does not lead to paired DNA damage--the characteristic of the transcribing fragment of the human ribosomal operon that allows its being detected in biological fluids at the death of different body cells].

It was shown by blot-hybridization with corresponding DNA probes after electrophoretic separation of control and experimental samples of human genome DNA that accumulation of single-strand breaks in the chains of double-strand fragment of transcribing range of ribosomal gene (TRrDNA) does not result in double-strand breaks. That differs from the other studied DNA sequences (cluster of histon genes, Alu-repetition, telomeric repetition and satellite III). Single-strand breaks and double-strand breaks were induced by endonucleases and by gamma-radiation.