Female-age-dependent changes in the lipid fingerprint of the mammalian oocytes.

Study Question: Can oocyte functionality be assessed by observing changes in their intracytoplasmic lipid droplets (LDs) profiles?

Summary Answer: Lipid profile changes can reliably be detected in human oocytes; lipid changes are linked with maternal age and impaired developmental competence in a mouse model.

What Is Known Already: In all cellular components, lipid damage is the earliest manifestation of oxidative stress (OS), which leads to a cascade of negative consequences for organelles and DNA. Lipid damage is marked by the accumulation of LDs.

Extended Inflammation Parameters (EIP) as Markers of Inflammation in Systemic Sclerosis.

Background: Systemic sclerosis (SSc) is an autoimmune disease characterized by inflammation, progressive vasculopathy, and fibrosis of skin and internal organs. The aim of the study was to evaluate extended inflammatory parameters (EIP) in patients with SSc in comparison to the control group of healthy subjects.

Methods: A total of 28 patients with SSc and 29 healthy controls (HCs) were included in the study.

European pollen reanalysis, 1980-2022, for alder, birch, and olive.

The dataset presents a 43 year-long reanalysis of pollen seasons for three major allergenic genera of trees in Europe: alder (Alnus), birch (Betula), and olive (Olea). Driven by the meteorological reanalysis ERA5, the atmospheric composition model SILAM predicted the flowering period and calculated the Europe-wide dispersion pattern of pollen for the years 1980-2022. The model applied an extended 4-dimensional variational data assimilation of in-situ observations of aerobiological networks in 34 European countries to reproduce the inter-annual variability and trends of pollen production and distribution.

Associations Between Every day and Medical Setting-Based Discrimination and Religious and Sociodemographic Characteristics of Muslim Americans: Findings from a National Survey.

We investigated the associations between sociodemographic factors, religiosity traits, and the perception of discrimination among Muslim Americans in both everyday life and medical settings. A self-administered web-based questionnaire, comprising validated measures of discrimination and religiosity, was completed by a convenience sample of English-speaking adult Muslim Americans, recruited through both in-person and online channels. Among the 1281 respondents, less than half were born in the USA (46%), and a significant portion displayed visible religious markers, such as wearing a hijab or having a beard (61%).

Proapoptotic and antimigration properties of osthole in combination with LY294002 against human glioma cells.

Anaplastic astrocytoma and glioblastoma multiforme are infiltrating and vascularized gliomas with a high degree of chemoresistance and metastasis. Our previous studies have shown that osthole may be of great importance in the treatment of gliomas. Therefore, in this work, for the first time, coumarin was used in combination with LY294002-an inhibitor of the PI3K-Akt/PKB-mTOR pathway, which is overly active in gliomas.

tuberculosis in a captive lion in Ukraine - Case Report.

. Because of the armed conflict in Ukraine, companion, farm and captive wild animals have been moved in a simplified procedure across the Polish-Ukrainian border. For that reason, in 2022, Poznań Zoo provided support for almost 200 wild animals before movement to facilities in other countries.

Into the dark of the black oesophagus.

Acute esophageal necrosis (AEN), known as black oesophagus or Gurvit's syndrome, is an extremely rare clinical syndrome. Patients usually present with life-threatening symptoms of upper gastrointestinal haemorrhage (70-90% of cases), as in this case report. Diagnosis of AEN is confirmed based on oesophagogastroduodenoscopy which reveals diffuse or patchy circumferential black necrotic oesophageal mucosa.

A case of exudative pleuritis caused by Staphylococcus warneri.

This paper discusses the case of a patient with exudative bacterial pneumonia caused by Staphylococcus warneri. The patient had been abusing alcohol and nicotine for several decades, which should be considered an immunosuppressive factor. Staphylococcus warneri is part of the skin microbiome, but can also be a pathogenic agent in certain circumstances.

Senior organizations at the time of SARS-CoV-2 pandemic. Part I.

Introduction And Objective: Senior Clubs are the most commonly available and, in most cases, free form of activation of older people. The aim of their functioning is improvement in the quality of life of seniors through education and activation. By using research tools to assess the needs of seniors it is possible to determine the direction of intervention related with an effective therapeutic, educational and activation procedures.

Unveiling the Tensor Vastus Intermedius - A distinct anatomical phenomenon or a standard variation? A comparative analysis of comprehensive literature and original cadaveric studies.

Introduction And Objective: The aim of this study was to enhance the understanding of the tensor vastus intermedius (TVI), a recently recognized addition to the quadriceps femoris muscle group, by conducting a systematic literature review with empirical cadaveric research to provide comprehensive data on its anatomical features, including dimensions, topography, variations, and prevalence.

Material And Methods: An exhaustive literature review was conducted using medical databases such as PubMed, MEDLINE, and Scopus. This review was complemented by original cadaveric research involving 30 autopsied adult bodies, encompassing 60 lower limbs, to empirically investigate the TVI's anatomical characteristics.

Job burnout in a sample of Polish paramedics - role of work experience, age and health behaviours.

Introduction And Objective: Job burnout is a phenomenon that results from unmanaged chronic workplace stress, which is directly related to the stressful nature or workplace, as well as experiencing chronic emotional strain and is characterized by high emotional exhaustion and depersonalization and low professional efficacy. Emergency Medical Teams are just such a workplace. Paramedics functioning in a state of prolonged, permanent stress begin to lose motivation to continue their work with their previous commitment.

Systematic review and meta-analysis of the association between young maternal age and fetal abnormalities.

Fetal anomalies among young women and adolescents pose major public health concerns. This systematic review aims to elucidate the relationship between young maternal age and the incidence of fetal abnormalities. According to the systematic review and meta-analysis PRISMA protocol, cohort, cross-sectional and case-control studies were scrutinized to include 80,393,450 participants across diverse regions.

Determination of spectroscopy marker of atherosclerotic carotid stenosis using FTIR-ATR combined with machine learning and chemometrics analyses.

Atherosclerotic carotid stenosis (ACS) is a recognized risk factor for ischemic stroke. Currently, the gold diagnostic standard is Doppler ultrasound, the results of which do not provide certainty whether a given person should be qualified for surgery or not, because in some patients, carotid artery stenosis, for example at the level of 70 %, does not cause ischemic stroke in others yes. Therefore, there is a need for new methods that will clearly indicate the marker qualifying the patient for surgery.

Raman spectroscopy combined with machine learning and chemometrics analyses as a tool for identification atherosclerotic carotid stenosis from serum.

Atherosclerosis carotid stenosis (ACS) is one of the main causes of stroke. Unfortunately, the highest number of people go to the doctor with an advanced disease or as a result of a stroke, because carotid atherosclerosis does not cause obvious symptoms. Therefore, it is important to find a diagnostic method to detect the disease during routine tests (using blood or serum).

Development of two ultra-sensitive UHPLC-QqQ-MS/MS methods for the simultaneous determination of hydroxyzine and its active metabolite (cetirizine) in human blood: applications to real cases of forensic toxicology.

Both postmortem toxicological and medical-forensic examinations are very important in the case of analyzing various types of chemical substances. Hydroxyzine (HZ) is a first-generation antihistamine drug with a sedative effect that disrupts cognitive function and affects the ability to drive motor vehicles. Enzymatic oxidation of the hydroxy-methyl group to the carboxyl group leads to the formation of its main metabolite-cetirizine (CZ).

Adsorptive Stripping Voltammetric Quercetin Determination in Pharmaceuticals and Urine Samples Using a Long Service-Life Array of Carbon Composite Microelectrodes.

This article presents for the first time a new working electrode with a long service life- the bismuth-plated array of carbon composite microelectrodes for the simple, fast and sensitive determination of quercetin by adsorptive stripping voltammetry. The main experimental conditions were selected. The calibration graph was linear from 1 × 10 to 2 × 10 mol L with an accumulation time of 60 s.

Quantitative Study of Vitamin K in Plants by Pressurized Liquid Extraction and LC-MS/MS.

The health-promoting properties of vitamin K stimulate the growing interest in this compound, which translates into the development of new analytical methodologies for its determination. New, more efficient methods of its isolation are sought, paying increasingly more attention to the methods within currently available extraction techniques that, owing to the optimization of the process, not only increase the extraction efficiency but are also economical and environmentally friendly. This article proposes a procedure for the extraction and analysis of one of the vitamin K vitamers, i.