5 results match your criteria: "Medical School of Chongqing University[Affiliation]"
Naked-eye detection of site-specific ssRNA and ssDNA using PAMmer-assisted CRISPR/Cas9 coupling with exponential amplification reaction.
Talanta
October 2021
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
Accurate and effective detection of single-stranded nucleic acids is vital in both disease diagnosis and pathological studies. Hence, we develop a PAMmer-assisted CRISPR/Cas9 system mediated G4-EXPAR (Cas-G4EX) strategy for site-specific detection of ssRNA and ssDNA. PAMmer-assisted CRISPR/Cas9 executes the site-specific cleavage of target ssRNA or ssDNA and released product fragment with the desired sequence at the 3'-terminal.
View Article and Find Full Text PDFDisposable 3D GNAs/AuNPs DNA-Circuit Strip for miRNAs Dynamic Quantification.
Small
October 2020
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing, 400044, China.
Article Synopsis
- Real-time monitoring of microRNAs (miRNAs) is crucial for diagnosis and therapy, and a new sensor using a graphene nanoarray/gold nanoparticles (GNAs/AuNPs) carbon paper electrode has been developed to improve this process.
- The sensor allows dynamic and sensitive analysis of miR-21 and miR-155, achieving quick detection within 16 minutes and low detection limits of 21.4 and 30.3 am, respectively.
- The new sensor shows comparable results to traditional qRT-PCR methods in clinical samples, indicating its reliability for point-of-care testing and monitoring of biomolecules.
Traditional Chinese and Western Medicines Jointly Beat COVID-19 Pandemic.
Chin J Integr Med
June 2020
Center of Smart Laboratory and Molecular Medicine, Medical School of Chongqing University, Chongqing, 400044, China.
Aptamer-Cholesterol-Mediated Proximity Ligation Assay for Accurate Identification of Exosomes.
Anal Chem
April 2020
Center for Smart Laboratory and Molecular Medicine, Medical School of Chongqing University, Chongqing, 400044, China.
Article Synopsis
- The study introduces an Aptamer-cholesterol-mediated Proximity Ligation Assay (AcmPLA) to accurately identify exosomes, crucial for disease diagnosis and therapy.* -
- This dual-probe strategy uses one aptamer to target the exosomal protein CD63 and a cholesterol probe for the biolipid layer, enabling specific recognition and enhanced signal detection through rolling circle amplification (RCA).* -
- The method allows for precise exosome isolation and identification, making it valuable for applications in biological science, biomedical engineering, and personalized medicine.*
Penetration of the blood-brain barrier and the anti-tumour effect of a novel PLGA-lysoGM1/DOX micelle drug delivery system.
Nanoscale
February 2020
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China. and Medical School of Chongqing University, Chongqing University, Chongqing 400044, China.
Effective treatment of glioma and other central nervous system (CNS) diseases is hindered by the presence of the blood-brain barrier (BBB). A novel nano-delivery vehicle system composed of PLGA-lysoGM1/DOX micelles was developed to cross the BBB for CNS treatment. We have shown that doxorubicin (DOX) as a model drug encapsulated in PLGA-lysoGM1 micelles can achieve up to 3.
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