Electropositive Magnetic Fluorescent Nanoprobe-Mediated Immunochromatographic Assay for the Ultrasensitive and Simultaneous Detection of Bacteria.

Immunochromatographic assays (ICAs) provide simple and rapid strategies for bacterial diagnosis but still suffer from the problems of low sensitivity and high dependency on paired antibodies. Herein, the broad-spectrum capture and detection capability of the antibody-free electropositive nanoprobe are clarified for bacteria for the first time and an ultrasensitive fluorescent ICA platform is constructed for the simultaneous diagnosis of multiple pathogens. A magnetic multilayer quantum dot nanocomposite with an amino-embedded SiO shell (MagMQD@Si) is designed to enrich bacteria from solutions effectively, offer high luminescence, and reduce background signals on test strips, thus greatly improving the sensitivity and stability of ICA technique for pathogen.

Safety and infection risk factors in elderly acute myeloid leukemia patients undergoing induction therapy with venetoclax combined with hypomethylating agents.

Objective: To retrospectively analyze the incidence of infections in elderly acute myeloid leukemia (AML) patients undergoing induction therapy with venetoclax combined with hypomethylating agents and to compare these findings with those from patients receiving standard or low-dose chemotherapy.

Methods: Medical records of 169 elderly (≥60 years old) AML patients diagnosed via MICM (morphology, immunology, cytogenetics, and molecular genetics) at the First Affiliated Hospital of USTC between June 2019 and June 2022 were reviewed. Patients were divided into three groups: venetoclax combined with hypomethylating agents group (targeted therapy group), standard chemotherapy group, and low-dose chemotherapy group.

Chinese guidelines for the diagnosis and treatment of rheumatoid arthritis: 2024 update.

Rheumatoid arthritis (RA) is an autoimmune disease with destructive arthritis as its main clinical manifestation, which is a major cause of disability. It is very important to formulate and update a guideline for the diagnosis and treatment of RA that adhere to international guideline development standards and can be applied to clinical practice in China. This guideline is endorsed and developed by the National Clinical Research Center for Dermatologic and Immunologic Diseases, collaborated with Rheumatologists Branch of Chinese Medical Doctor Association, Rheumatology Rehabilitation Branch of Chinese Association of Rehabilitation Medicine, Rheumatology Branch of Chinese Research Hospital Association, and Rheumatology Branch of Beijing Association of Holistic Integrative Medicine, based on grading of recommendations assessment, development and evaluation (GRADE) and reporting items for practice guidelines in healthcare (RIGHT).

Electrochemical Detection of Hydrogen Peroxide at High Concentrations Using Hastelloy G35 Electrode.

The detection of hydrogen peroxide (HO) at elevated concentrations while eliminating oxygen interference presents a significant challenge. Nickel-based stainless steel, such as Hastelloy G35, has shown excellent corrosion resistance. However, it has never been used in electroanalysis.

Sibanin, a novel black fly-derived Kunitz protease inhibitor, prevents thrombus formation in mice by anticoagulation-antiplatelet duality.

Most Kunitz inhibitors exhibit serine protease inhibitory activity, but limited information is available on the regulation of platelet function. Herein, we report the purification and characterization of a novel single Kunitz domain inhibitor (Sibanin) from the salivary glands of the black fly Simulium bannaense. Recombinant Sibanin prolonged activated partial thromboplastin time and prothrombin time, and exhibited high-affinity binding to FXa and elastase with a KD of 5.

A combination of genome mining with OSMAC strategy facilitates the discovery of bioactive metabolites produced from termite-associated Streptomyces tanashiensis BYF-112.

Background: Previously, eight new alkaloids were obtained from the fermentation extract of termite-associated Streptomyces tanashiensis BYF-112. However, genome analysis indicated the presence of many undiscovered secondary metabolites in S. tanashiensis BYF-112.

The Physical Developmental Characterization of Children with Nutritional Deficiencies and Attributed Specific Categories.

Background: There are few studies examining the physical developmental phenotypes of nutritional deficiency diseases (NDDs) among Chinese children aged 1-7 years by anthropometrics and clarifying the specific NDD categories that caused growth faltering.

Methods: A total of 3054 cases of NDDs in children aged 1-7 years were investigated. The age, height, and weight of children with NDDs were adjusted by using the skewness coefficient-median-coefficient of variation method, and the results were compared with the WHO standardized level.

ATAD1 Regulates Neuronal Development and Synapse Formation Through Tuning Mitochondrial Function.

Mitochondrial function is essential for synaptic function. ATAD1, an AAA+ protease involved in mitochondrial quality control, governs fission-fusion dynamics within the organelle. However, the distribution and functional role of ATAD1 in neurons remain poorly understood.

Antifungal Activity of Genistein Against Phytopathogenic Fungi Through ROS-Mediated Lipid Peroxidation.

() is a necrotrophic fungus responsible for apple Valsa canker, which significantly diminishes apple production yields and quality in China. Our serendipitous findings revealed that genistein significantly inhibits the mycelial growth of , with an inhibition rate reaching 42.36 ± 3.

Endothelial IGFBP6 suppresses vascular inflammation and atherosclerosis.

Beyond dyslipidemia, inflammation contributes to the development of atherosclerosis. However, intrinsic factors that counteract vascular inflammation and atherosclerosis remain scarce. Here we identify insulin-like growth factor binding protein 6 (IGFBP6) as a homeostasis-associated molecule that restrains endothelial inflammation and atherosclerosis.

Distinction in the function and microstructure of white matter between major depressive disorder and generalized anxiety disorder.

Background: Major depressive disorder (MDD) and generalized anxiety disorder (GAD) are two of the leading causes of impairment to human mental health. These two psychiatric disorders overlap in many symptoms and neurobiological features thus difficult to distinguish in some cases.

Methods: We enrolled 102 participants, comprising 40 patients with MDD, 32 patients with GAD and 30 matched healthy controls (HCs), to undergo multimodal magnetic resonance imaging (MRI) scans.

Bridging thrombolysis versus direct endovascular treatment in acute vertebrobasilar artery complex occlusion.

Objective: Endovascular treatment (EVT) is an effective treatment for patients with acute vertebrobasilar artery complex occlusion (VBAO). However, the benefit of bridging thrombolysis prior to EVT remains controversial. The purpose of the present study is to explore the best treatment strategy between bridging treatment (BT) and direct EVT in patients with acute VBAO.

Prediction of Thermodynamic Properties of C-Based Fullerenols Using Machine Learning.

Traditional machine learning methods face significant challenges in predicting the properties of highly symmetric molecules. In this study, we developed a machine learning model based on graph neural networks (GNNs) to accurately and swiftly predict the thermodynamic and photochemical properties of fullerenols, such as C(OH) ( = 1 to 30). First, we established a global method for generating fullerenol isomers through isomer fingerprinting, which can generate all possible isomers or produce diverse structural types on demand.

Therapeutic Potential of Carbon Dots Derived from Phytochemicals as Nanozymes Exhibiting Superoxide Dismutase Activity for Anemia.

Anemia is a potentially life-threatening blood disorder caused by an insufficient erythroblast volume in the circulatory system. Self-renewal failure of erythroblast progenitors is one of the key pathological factors leading to erythroblast deficiency. However, there are currently no effective drugs that selectively target this process.

Effects of nanosecond‑ and microsecond‑pulse Er, Cr: YSGG laser on the morphology and pulp temperature of dentin in dental restoration debonding.

Objective: This study evaluated dentin morphology and pulp cavity temperature changes during nanosecond‑ and microsecond‑pulse Er, Cr: YSGG laser debonding restoration and residual adhesive.

Materials And Methods: Ten caries-free teeth had their enamel removed perpendicular to the long axis, followed by bonding of glass ceramic restorations. The samples were randomly divided into two groups and subjected to Er, Cr: YSGG laser (3 mJ, 100 Hz, 100 ns), (3 mJ, 100 Hz, 150 µs) for debonding of restoration and residual adhesive on dentin surfaces.

Age-specific and sex-specific associations of visceral adipose tissue with metabolic health status and cardiovascular disease risk.

Background: Visceral adipose tissue (VAT) is known to play a role in the development of metabolic and cardiovascular disease (CVD). However, the age- and sex-specific associations between VAT and these diseases remain unclear.

Methods: In this cross-sectional study, 1,150 participants (39.

Structural and load-dependent arterial stiffness across the adult life span.

Background: The arterial stiffening is attributed to the intrinsic structural stiffening and/or load-dependent stiffening by increased blood pressure (BP). The respective lifetime alterations and major determinants of the two components with normal aging are not clear.

Methods: A total of 3053 healthy adults (1922 women) aged 18-79 years were enrolled.

Claudin 18.2-targeting antibody-drug conjugate CMG901 in patients with advanced gastric or gastro-oesophageal junction cancer (KYM901): a multicentre, open-label, single-arm, phase 1 trial.

Background: CMG901 is a novel first-in-class antibody-drug conjugate with a humanised anticlaudin 18.2 antibody linked to microtubule-disrupting agent monomethyl auristatin E. We aimed to assess the antitumour activity and safety of CMG901 in patients with advanced gastric or gastro-oesophageal junction cancer and other solid tumours.

Facile fabrication of sensing electrode based on CoFe-MOFs/MXene for ultrasensitive detection of picomolar chloramphenicol.

Precise detection of ultralow-level antibiotics, such as picomole, in aqueous environments is significant for human health, however, it presents a great challenge to the adsorption capacity and electrocatalytic ability of sensing materials. Here, we used a one-step hydrothermal method to in situ grow spindle-like CoFe-based metal-organic frameworks (MOFs) with a size of about 50 nm in the region of hydrophilic MXene-loading hydrophobic carbon paper. By combining MOFs with abundant adsorption sites and MXene with high conductivity, the problems of adsorption and electrons transfer of ultralow-level antibiotics have been solved, and achieving precise detection of picomole-level antibiotics.

Chemical profile changes in Peanut seeds infected with aspergillus flavus via widely targeted metabolomics.

Peanut seeds are enriched with protein and fatty acids, making them susceptible to infection by Aspergillus flavus (A. flavus). The infected seeds are harmful to human health due to the aflatoxin contamination.

ATG9 promotes autophagosome formation through interaction with LC3.

The autophagosome is a double-membrane organelle that executes macroautophagy. Previous studies have shown that the autophagosome formation is driven by autophagy-related genes, among which ATG9 is the only conserved transmembrane protein and has been shown to play a critical role in the autophagosome formation. However, how ATG9 binds to the growing autophagosome membrane has remained uncertain.

Effect of eHealth Interventions on Body Image of Patients With Cancer: Systematic Review.

Background: Body image issues are prevalent among individuals diagnosed with cancer, leading to detrimental effects on their physical and psychological recovery. eHealth has emerged as a promising approach for enhancing the body image of patients with cancer.

Objective: The purpose of this study was to evaluate the effectiveness of eHealth interventions on body image and other health outcomes (quality of life, physical symptoms, and emotional distress) among patients with cancer.

Prognostic and therapeutic implications of measurable residual disease levels during remission induction of childhood ALL.

We evaluated the prognostic and therapeutic significance of measurable residual disease (MRD) during remission induction in pediatric acute lymphoblastic leukemia (ALL) patients. In the CCCG-ALL-2015 protocol, 7640 patients were categorized into low-, intermediate-, or high-risk groups based on clinical and genetic features. Final risk classification was determined by MRD assessed via flow cytometry on Days 19 and 46 of remission induction, with additional intensified chemotherapy for Day 19 MRD ≥1%.