Tracing active members in microbial communities by BONCAT and click chemistry-based enrichment of newly synthesized proteins.

A comprehensive understanding of microbial community dynamics is fundamental to the advancement of environmental microbiology, human health, and biotechnology. Metaproteomics, defined as the analysis of all proteins present within a microbial community, provides insights into these complex systems. Microbial adaptation and activity depend to an important extent on newly synthesized proteins (nP), however, the distinction between nP and bulk proteins is challenging.

sp. nov., a Gram-positive mesophilic bacterium isolated from plant lesions.

A bacterial strain was isolated from pathogenic lesions of tree leaves from the Teutoburg Forest in North Rhine-Westphalia, Germany, by culture on non-selective agar plates. 16S rRNA sequencing revealed 100% similarity to and , as well as 99% similarity to and . Here, we used genome-based taxonomy with the Type (Strain) Genome Server (TYGS), which suggests the isolation of a novel prokaryotic strain.

Transcriptomic analysis and cellular responses to nanoscale zero-valent iron in green microalga Raphidocelis subcapitata.

Nanoscale zero valent iron (nZVI) is used to remediate aquifers polluted by organochlorines or heavy metals and was also suggested to eliminate harmful algal blooms. nZVI can therefore affect microorganisms in the vicinity of the application area, including microalgae. However, studies on early transcriptomic effects of microalgae after exposure to nZVI are rare.

Circularly assembled genome sequences of sp. strain MM231 and MM232, isolated from a pond at Bielefeld University campus.

Photosynthetic organisms often interact with heterotrophic microbes. We here report the complete genome sequences of the bacterial strains sp. MM231 and MM232.

Complete genome sequences of sp. strain MM2321 and sp. strain MM2322, isolated from a sand sample in Harsewinkel, Germany.

We cultivated bacteria contained in a sandy soil sample, isolated DNA from a single bacterial colony, and assembled from genomic reads the full genome sequence of and strains, termed MM2321 and MM2322. Besides the genome sequences, the phylogenetic classifications of both strains are reported.

Sigma Factor Engineering in sp. SE50/110: Expression of the Alternative Sigma Factor Gene (σH) Enhances Acarbose Yield and Alters Cell Morphology.

Sigma factors are transcriptional regulators that are part of complex regulatory networks for major cellular processes, as well as for growth phase-dependent regulation and stress response. sp. SE50/110 is the natural producer of acarbose, an α-glucosidase inhibitor that is used in diabetes type 2 treatment.

Early-life factors shaping the gut microbiota of Common buzzard nestlings.

Background: Exploring the dynamics of gut microbiome colonisation during early-life stages is important for understanding the potential impact of microbes on host development and fitness. Evidence from model organisms suggests a crucial early-life phase when shifts in gut microbiota can lead to immune dysregulation and reduced host condition. However, our understanding of gut microbiota colonisation in long-lived vertebrates, especially during early development, remains limited.

Isolation, characterisation and description of the roseoflavin producer Streptomyces berlinensis sp. nov.

The Gram-positive bacteria Streptomyces davaonensis and Streptomyces cinnabarinus have been the only organisms known to produce roseoflavin, a riboflavin (vitamin B) derived red antibiotic. Using a selective growth medium and a phenotypic screening, we were able to isolate a novel roseoflavin producer from a German soil sample. The isolation procedure was repeated twice, that is, the same strain could be isolated from the same location in Berlin 6 months and 12 months after its first isolation.

sp. nov. and sp. nov., originating from chilled beef and chicken breast.

By investigating wet and dry age-related ripening of beef, strains V3/3/4/13 and V3/K/3/5 were isolated. Strain V3/3/4/13 exhibited more than 99 % 16S rRNA gene-based similarity to and other members of this group, while isolate V3/K/3/5 was very close to and a number of relatives within the group. Additional comparisons of complete sequences and draft genomes allowed us to place isolate V3/3/4/13 close to DSM 26521.

Mobile applications in adolescent psychotherapy during the COVID-19 pandemic: a systematic review.

Background: To bridge the gap in adolescent psychotherapy created by the increasing need for mental health interventions and the limited possibilities of in-person treatment during the pandemic, many health care providers opted to offer online mental health care programs. As a result, the number of mental health apps available in app stores experienced a sharp increase during the COVID-19 pandemic.

Objective: The aim of the current review is to provide an overview of feasibility and effectiveness studies testing mobile applications in adolescent psychotherapy during the peak phase of the COVID-19 pandemic.

Microbial Diversity and Community Structure of Wastewater-Driven Microalgal Biofilms.

Dwindling water sources increase the need for efficient wastewater treatment. Solar-driven algal turf scrubber (ATS) system may remediate wastewater by supporting the development and growth of periphytic microbiomes that function and interact in a highly dynamic manner through symbiotic interactions. Using ITS and 16S rRNA gene amplicon sequencing, we profiled the microbial communities of four microbial biofilms from ATS systems operated with municipal wastewater (mWW), diluted cattle and pig manure (CattleM and PigM), and biogas plant effluent supernatant (BGE) in comparison to the initial inocula and the respective wastewater substrates.

Can Genome Sequencing Coupled to Flux Balance Analyses Offer Precision Guidance for Industrial Strain Development? The Lessons from Carbon Trafficking in ATCC 21573.

Systems biology tools offer new prospects for industrial strain selection. For bacteria that are significant for industrial applications, whole-genome sequencing coupled to flux balance analysis (FBA) can help unpack the complex relationships between genome mutations and carbon trafficking. This work investigates the l-tyrosine (l-Tyr) overproducing model system ATCC 21573 with an eye to more rational and precision strain development.

Environmental Burden of Disease due to Emissions of Hard Coal- and Lignite-Fired Power Plants in Germany.

The study estimated the environmental burden of disease (EBD) attributable to a long-term exposure of the population to nitrogen dioxide (NO) and fine particulate matter (PM) emissions from hard coal- and lignite-fired power plants in Germany for the year 2015. The contribution of coal-fired power plants to the total air pollutant concentration was modelled using a chemical transport model and then combined with population data to assess the corresponding population exposure. We calculated years of life lost (YLL), years of life with disability, or disability-adjusted life years for different health outcomes with a strong evidence for an association with the exposure.

CYP19A1 mediates severe SARS-CoV-2 disease outcome in males.

Male sex represents one of the major risk factors for severe COVID-19 outcome. However, underlying mechanisms that mediate sex-dependent disease outcome are as yet unknown. Here, we identify the CYP19A1 gene encoding for the testosterone-to-estradiol metabolizing enzyme CYP19A1 (also known as aromatase) as a host factor that contributes to worsened disease outcome in SARS-CoV-2-infected males.

Exploring engineered vesiculation by Pseudomonas putida KT2440 for natural product biosynthesis.

Pseudomonas species have become promising cell factories for the production of natural products due to their inherent robustness. Although these bacteria have naturally evolved strategies to cope with different kinds of stress, many biotechnological applications benefit from engineering of optimised chassis strains with specially adapted tolerance traits. Here, we explored the formation of outer membrane vesicles (OMV) of Pseudomonas putida KT2440.

Impact of COVID-19-adapted guidelines using different airway management strategies on resuscitation quality in out-of-hospital-cardiac-arrest - a randomised manikin study.

Background: Although airway management for paramedics has moved away from endotracheal intubation towards extraglottic airway devices in recent years, in the context of COVID-19, endotracheal intubation has seen a revival. Endotracheal intubation has been recommended again under the assumption that it provides better protection against aerosol liberation and infection risk for care providers than extraglottic airway devices accepting an increase in no-flow time and possibly worsen patient outcomes.

Methods: In this manikin study paramedics performed advanced cardiac life support with non-shockable (Non-VF) and shockable rhythms (VF) in four settings: ERC guidelines 2021 (control), COVID-19-guidelines using videolaryngoscopic intubation (COVID-19-intubation), laryngeal mask (COVID-19-Laryngeal-Mask) or a modified laryngeal mask modified with a shower cap (COVID-19-showercap) to reduce aerosol liberation simulated by a fog machine.

Complete Genome Sequence of the Carboxydotrophic Knallgas Bacterium Pseudomonas carboxydohydrogena Strain DSM 1083.

Pseudomonas carboxydohydrogena is a lithoautotrophic and obligate aerobic alphaproteobacterium, which has the unique ability to utilize CO, CO, H, and mixtures thereof as sole carbon and energy sources. Here, we report the complete genome sequence of type strain DSM 1083 and its close relation to Afipia carboxidovorans strain OM5.

Overlapping SigH and SigE sigma factor regulons in .

The sigma H (σ) and sigma E (σ) subunits of RNA polymerase belong to Group 4 of sigma factors, also called extracytoplasmic function (ECF) sigma factors. Genes of the σ regulon that are involved in heat and oxidative stress response have already been defined, whereas the genes of the σ regulon, which is involved in cell surface stress response, have not been explored until now. Using the RES167 strain and its derivative Δ with a deletion in the anti-σ gene, differential gene expression was analyzed by RNA sequencing.