BANF1 promotes glutamate-induced apoptosis of HT-22 hippocampal neurons.

Background: Glutamate exposure was fatal to HT-22 neuronal cells that derived from mouse hippocampus. This is often used as a model for hippocampus neurodegeneration in vitro. The targets relevant to glutamate-induced neuronal toxicity is not fully understood.

Which gait training intervention can most effectively improve gait ability in patients with cerebral palsy? A systematic review and network meta-analysis.

Background: A vital objective to treat people with cerebral palsy (CP) is to increase gait velocity and improve gross motor function. This study aimed to evaluate the relative effectiveness of gait training interventions for persons with CP.

Methods: Studies published up to October 26, 2022 were searched from four electronic databases [including Medline ( PubMed), Web of Science, Embase and Cochrane].

STAT3 exerts pro-tumor and anti-autophagy roles in cervical cancer.

Background: STAT3 plays an important role in cervical cancer. LC3B, the most potential molecular biomarker of autophagy that may promote or inhibit cancer progression, can be downregulated by STAT3. However the role of STAT3 in the autophagy of cervical cancer remains unclear.

Copy number variation analysis of m A regulators identified METTL3 as a prognostic and immune-related biomarker in bladder cancer.

Purpose: Growing evidence has demonstrated an indispensable role for N -methyladenosine (m A) in human diseases, but the copy number variations (CNVs) of m A regulatory genes in bladder cancer (BLCA) remains largely unknown.

Methods: We investigated the CNVs on all known m A regulatory genes using the Cancer Genome Atlas (TCGA) database. The association between CNV events and clinicopathological as well as molecular characteristics of BLCA patients were explored.

Fatty acid β-oxidation promotes breast cancer stemness and metastasis via the miRNA-328-3p-CPT1A pathway.

MicroRNAs (miRNA) have been shown to be associated with tumor diagnosis, prognosis, and therapeutic response. MiR-328-3p plays a significant role in breast cancer growth; however, its actual function and how it modulates specific biological functions is poorly understood. Here, miR-328-3p was significantly downregulated in breast cancer, especially in patients with metastasis.

Metabolic syndrome and the incidence of knee osteoarthritis: A meta-analysis of prospective cohort studies.

Background: Cross-sectional studies suggest an association between metabolic syndrome (MetS) and knee osteoarthritis (KOA). We performed a meta-analysis to evaluate whether MetS is an independent risk factor for KOA.

Methods: Prospective cohort studies evaluating the association between MetS and KOA in general population were retrieved from PubMed and Embase.

Multi-omics analysis of copy number variations of RNA regulatory genes in soft tissue sarcoma.

Aims: RNA regulatory genes were closely associated with tumorigenesis and prognosis in multiple tumors. Copy number variation (CNV) is a frequent characteristic in soft tissue sarcomas (STS). However, little is known regarding their possible roles in STS.

Bioinformatics analysis integrating metabolomics of mA RNA microarray in intervertebral disc degeneration.

To explore the potential functions and mechanism of .methyladenosine (mA) abnormality of RNAs in nucleus pulposus from the intervertebral disc degeneration (IDD). We performed rat model, mA epitranscriptomic microarray, bioinformatics analysis and metabolomics.

Exendin-4 attenuates cardiac hypertrophy via AMPK/mTOR signaling pathway activation.

Diabetes mellitus is a prominent risk factor for cardiovascular diseases. Diabetic cardiomyopathy is an important complication of the heart independent of hypertension and coronary artery disease and is accompanied by cardiac hypertrophy. Cardiac hypertrophy easily leads to heart failure, which is a leading cause of morbidity and mortality.

Computed tomography-guided sub-end plate injection of pingyangmycin for a novel rabbit model of slowly progressive disc degeneration.

Background Context: Different animal models are used in disc degenerative disease research by now. To our knowledge, a functional animal model that mimics ischemic and slowly progressive disc degeneration of humans does not exist.

Study Design: This is an experimental animal study of disc degeneration.

Correlation of viral loads with HCV genotypes: higher levels of virus were revealed among blood donors infected with 6a strains.

Background: Both HCV genotypes and viral loads are predictors of therapeutic outcomes among patients treated with α-interferon plus ribavirin; however, such correlation has only been studied for genotypes 1, 2, and 3 but not for genotype 6.

Methodology/findings: 299 voluntary blood donors were recruited who were HCV viremic. Their mean age was 31.

HCV 6a prevalence in Guangdong province had the origin from Vietnam and recent dissemination to other regions of China: phylogeographic analyses.

Background: Recently in China, HCV 6a infection has shown a fast increase among patients and blood donors, possibly due to IDU linked transmission.

Methodology/findings: We recruited 210 drug users in Shanwei city, Guangdong province. Among them, HCV RNA was detected in 150 (71.

[Effects of caloric restriction on the oxidative stress injury and the expression of SIRT3 in PC12 cell].

Objective: To study the regulation effects of CR (caloric restriction) and SIRT3 in the H2O2-induced oxidative stress injury of PC12 cell.

Methods: The cells were divided into four groups: H2O2, H2O2 + CR, CR and control (high glucose). For control and H2O2 group, cells were cultured in DMEM containing 0.

[Subcellular mimical localization of the ATP synthase B subunit from Clonorchis sinensis under different conditions of cell cycling].

Objective: To investigate the subcellular localization of ATP synthase b subunit from Clonorchis sinensis under different conditions of Hela cell cycling, and the effect of this protein on the expression of its encoding-gene and homologous host genes.

Methods: pEGFP-N1-CsATP-synt_B and the vector pEGFP-N1 were transfected into Hela cells, respectively. Transfected cells were synchronized in G0/G1 by serum starvation, G1/S, S cells by double thymidine block, and G2/M cells by thymidine-Nocodozale block.

[A new method for calculating paternity index].

Objective: To introduce a new method for calculating the paternity index (PI).

Methods: Assuming that each allele from parents has undergone a transition before it segregates and transmits to child. The transition probability is 1 when parent allele is the same as child's, the transition probability is 0 when parent allele is different from the child's.

[Cloning and expression of the F0-ATP synthase B chain of Clonorchis sinensis and immunogenicity identification of the recombinant protein].

Objective: To clone and express the Clonorchis sinensis F0-ATP synthase b chain (CsF0-ATP-synt_B) gene and analyze immunogenicity of the recombinant protein.

Methods: The coding region F0-ATP synthase b chain gene with the mitochondrial targeting sequence (MTS) removed was amplified with PCR using the cloned plasmid as template, and the product was cloned into the prokaryotic expression vector pET-28a(+), transformed into E. coli BL21 (DE3) and induced with IPTG.

[The effects of heroin on intracellular free Ca2+ of rat myocardium].

Objective: To observe the effects of heroin on intracellular free Ca2+ in rat myocardium.

Methods: The effects of heroin on intracellular free Ca2+ were observed in cultured neonatal rat myocardium by measuring intracellular free Ca2+ concentration using calcium fluorescent probe Flou-3/AM and laser scanning confocal microscope.

Results: Different doses and concentrations of heroin appeared to have different effects on intracellular free Ca2+ concentrations, with a dosage dependent short linear increase in the fluorescence intensity (i.

[Cloning, expression and antibacterial activity of histone H1 and its N-terminal peptide from Carassius auratus].

In order to determine whether H1 histone proteins are associated with innate immune antimicrobial response in goldfish, we extracted the total RNA from the hemocytes of goldfish (Carassius auratus), designed 3 pairs of primers based on the previous antimicrobial peptide sequences from fish and performed RT-PCR. Among 3 obtained-PCR products, we identified a novel histone H1 coding sequence of 576 bp which belongs to the histone H1 family and is 78% homologous with the amino acid sequence of histone H1 from Salmon salar that had been found with an important role in salmon defenses against infectious pathogens. The H1 histone of goldfish contained 3 predicting cleavage sites that divided the protein into 4 parts.

[Pathologic changes of the cardiac conduction system in 12 patients with abnormal ECG].

Objective: To investigate the relationship between abnormal ECG and pathologic changes in the cardiac conduction system (CCS).

Method: Pathological changes of the CCS in 12 cases with abnormal ECG out of 16 pre-death ECG were observed.

Results: (1) Among 7 cases of sudden cardiac death, ECG monitoring recorded bradyarrhythmia in 6 cases, tachyarrhythmia 6 cases, bradycardia-tachycardia syndrome 2 cases, conduction block 6 cases, atrial premature beats 6 cases, ventricular premature beats 6 cases, and ST-T changes 4 cases.

[Following the fate of murine epidermal stem cells in a syngeneic dermal equivalent in vivo].

Objective: To follow the fate of murine epidermal stem cells (ESCs) seeded in a syngeneic dermal equivalent implanted in vivo.

Methods: Embryonic stem (ES) cells were induced in vitro to differentiate into ESCs. After stained with a fluorescent dye Hoechst 33342, these ESCs were seeded into a fibroblast-collagen-gelatin sponge complex, functioning as a dermal equivalent model, and implanted subcutaneously into 129/J mice, which were syngeneic to these stem cells.

[Diversity of paternity index with different allele frequencies].

Objective: To evaluate the diversity of combined paternity index (CPI) of multiple STR loci when different population allele frequencies was used to calculate the paternity index.

Methods: CPI of 13 CODIS (combined DNA index system) loci for 108 trio cases and 108 duo cases selected randomly were calculated by using five Chinese Han population allele frequencies, respectively.

Results: The CPI range for trio cases and duo cases were 2077.

Protective effect of membrane cofactor protein against complement-dependent injury.

Aim: To evaluate the protective role of membrane cofactor protein (MCP, CD46) on complement-dependent injury.

Methods: MCP was separated by ion exchange chromatography on a DEAE sephadex A-50 column from pig erythrocyte ghosts. Its protective effect was tested in models such as cobra venom factor (CVF)-induced platelet metamorphosis and aggregation, human serum-induced injury in isolated working guinea pig heart and reverse passive Arthus reaction.

[A study on the relationship between the degradation of protein and the postmortem interval].

Objective: To observe the degradation of actin and tubulin in the liver tissue of rats after death and to find an objective indicator of the postmortem interval (PMI).

Methods: Female rats were killed under anesthesia by ether and incubated at 21 degrees C in a temperature controlled system to simulate postmortem changes for 18 days postmortem. Protein in the hepatic tissue was extracted, actin and tubulin were then examined by western blot.

[A preliminary study on estimation of postmortem interval according to beta-actin mRNA stability in rat].

Objective: In order to find a new parameter to estimate the postmortem interval, beta-actin mRNA in lung and thoracic muscle of rats was detected at different time point postmortem.

Methods: Rats were killed by neck dislocation and left in a temperature controlling system at 21 degrees C for 12 days postmortem. Total RNA in lung and thoracic muscle at different time point was extracted and beta-actin mRNA expression was examined by RT-PCR.