Rho kinase regulates neurite outgrowth of hippocampal neurons via calcium dependent cytoskeleton regulation.

Objective: To investigate whether calcium is involved in downstream signal transduction in neurite outgrowth regulated by Rho kinase.

Methods: primary hippocampal neurons were cultured and treated with Rho kinase agonist (LPA) or antagonist (Y-27632). Then, the cytoskeleton and neurite outgrowth were observed.

CRMPs colocalize and interact with cytoskeleton in hippocampal neurons.

CRMP family proteins (CRMPs) are widely expressed in the developing neurons, mediating a variety of fundamental functions such as growth cone guidance, neuronal polarity and axon elongation. However, whether all the CRMP proteins interact with cytoskeleton remains unknown. In this study, we found that in cultured hippocampal neurons, CRMPs mainly colocalized with tubulin and actin network in neurites.

The association between B vitamins supplementation and adverse cardiovascular events: a meta-analysis.

This study is to explore the association of adverse cardiovascular events with B vitamins supplementation. Rev.Man 5.

CRMP-5 interacts with tubulin to promote growth cone development in neurons.

Collapsin response mediator proteins (CRMPs) have been reported to control axonal guidance during neuronal development and degeneration. Among these proteins, CRMP-5 has been indicated to play an important role in growth cone development. However, the mechanisms underlying the linkage between growth cone development and the cytoskeleton remain to be elucidated.