[Effects of PCB153 on oxidative stress and 8-OHdG content induced by PBDE-47 in human neuroblastoma cells in vitro].

Objective: To explore the effects of PCB153 (2,2,4,4,5,5-hexachlorobiphenyl) on oxidative stress and 8-OHdG (8-hydroxy-2'-deoxyguanosine) content induced by PBDE-47 (2,2,4,4-tetrabromodiphenyl ether) in SH-SY5Y cells.

Methods: SH-SY5Y cells were incubated with different concentrations of 1, 5, 10 micromol/L PBDE-47 or/and 5 micromol/L PCB153 and antioxidant N-acetyl cysteine (NAC 100 micromol/L) for 24h in vitro, ROS (reactive oxygen species) level and 8-OHdG were measured by using DCFH-DA fluorescent marking method and the skill of high performance liquid chromatogram-electrochemistry (HPLC-EC), respectively.

Results: As for ROS formation, there was no significant difference in PBDE-47 groups alone compared to the control group and their corresponding combined groups, respectively (P > 0.