[Effect of various storage methods on the viability of myxobacteria].

Viability of myxobacteria strains Myxococcus xanthus UNCM 10041, Polyangium cellulosum UNCM 10043, Archangium gephyra UNCM 10001 stored by the methods of lyophilization, cryoconservation, drying on paper discs, suspending in distilled water or physiological solution under a layer of mineral oil. The best results on viability preservation of all three studied strains were obtained when using the method of cryoconservation. It has been shown that the strains M.

Biokinetics of transdermal therapeutic medicinal form of phenazepam.

We studied the rate of phenazepam absorption into the blood and its transport to the brain from a transdermal therapeutic system and bioavailability of the drug in this system. Hydrogel matrix consisting of polyvinyl alcohol and 1,2-propylene glycol was used for application. Transdermal application of 0.

[Effect of DDT on the interaction of cortisol with glucocorticoid-binding proteins in rat brain].

It was established that DDT is the isosteric inhibitor of cortisol interaction with purified rat brain glucocorticoid-binding proteins. These results were confirmed in the experiments in vivo on Vistar female rats from one generation.

In vitro effects of corticosteroids, DDT, and 4, 9-dichlorodibenzodioxin on rat liver xanthine oxidase activity. Interactions between xanthine oxidase and cytochrome P450 in rat liver in vivo.

It was established that deoxycorticosterone, cortisol, dexamethasone, DDT, and 4,9-dichlorodibenzodioxin inhibit in vitro binding of xanthine to highly purified rat liver xanthine oxidase. They are suggested to be allosteric inhibitors. The corresponding inhibition and binding constants were estimated.

[Degradation of 4,9-dichlorodibenzodioxin in rat liver microsomes].

The rat liver microsomal cytochrome P 450 was established to dechlorinate 4,9-dichlorodibenzodioxin into 4,9-dihydroxydibenzodioxin. Two isoforms of this cytochrome P 450 were electrophoreticaly identified and quantified. 4,9-dihydroxidibenzodioxin transforms into resorcin with the help of ascorbic acid.

[Effect of reducing agents-antioxidants on rat uterine aromatase activity in vitro].

The effect of different reductors-antioxidants (ascorbic acid, cysteine, glutathione-SH and hydroquinone) on purified rat uterine aromatase activity was studied. It was established that all these compounds were aromatase activators. The possible mechanisms of this activation were discussed.

[The postradiation use of vitamin-containing complexes and a phycocyanin extract in a radiation lesion in rats].

Wistar rats have been exposed to X-rays with a dose of 5 Gy. Significant decrease in dehydrogenase activity, energy-rich phosphate level and efficiency of antioxidant defence and significant increase in pyruvate amount were observed within 4 weeks. It was also found that the feeding of exposed rats with phycocyanin extract from blue-green algae Spirulina platensis lead to correcting effect.

[Effect of some antioxidants and caffeine on xanthine dehydrogenase activity in rat liver in vitro].

The regulation of xanthine dehydrogenase activity by reductors-antioxidants (ascorbic acid, glutatione-SH, gentathione dithiothreitol, cysteine and hydrocortisone) and caffeine was investigated in the purified enzymatic preparation in vitro. It was shown, that reductors-antioxidants were incompetitor inhibitors of xanthine dehydrogenase and caffeine was a competitor-inhibitor of it. The mechanisms of inhibition of xanthine dehydrogenase activity by these agents were discussed.

[The gene-enzyme system of alcohol dehydrogenase during genotype changes in Drosophila melanogaster].

It is established, that certain variants of replacement of chromosomes performed on wild (C-S, D) and mutant (cn, vg) lines as well as continuous backcrosses, leads to the change of heat resistance and activity of F- and S-allozymes of ADH in tissues of synthesized forms of drosophila. But nevertheless, the electrophoretic mobility of allozymes does not change. It is assumed that post-translated modifications of ADH play the important part in processes of phylogenetic adaptation of Drosophila melanogaster.

[In vitro study of regulation of rat liver xanthine oxidase activity by antioxidant reducing agents].

It was learned the regulation of xanthine oxidase activity from rat liver in the partly purified prepared by ascorbic acid, glutathione-SH, dithiothreitol, cysteine++ and hydrocortisone++. It was shown that ascorbic acid glutathione-SH, dithiothreitol, and cysteine++ can be activators and uncompetitor inhibitors of xanthine oxidase in dependence from concentration. As far as hydrocortisone is concerned, it is a powerful uncompetitor inhibitor of xanthine oxidase, that is bind with it.

[Genotypic basis of low viability in vestigial mutants of Drosophila melanogaster].

The role of a marker mutation and other genes in a decrease in viability was studied in the Drosophila melanogaster vg line. In flies of the C-S line, chromosome 2 was substituted by the homologous chromosome of the vg flies. In addition, the flies of the mutant phenotype with mutant genes partially or completely substituted by the wild-type C-S genes were obtained in saturating crosses C-S x vg.

[Effect of caffeine on xanthine oxidase activity].

The effect of caffeine on the xanthine oxidase activity in human organism has been studied. It was revealed that caffeine calls the inconsiderable reliable increase of the level of uric acid and the reliable lowering of levels of hypoxanthine and xanthine in urine. The isosteric inhibition of xanthine oxidase activity by caffeine was revealed in the experiments in vitro.

The role of the sodium cycle of energy coupling in the emergence and persistence of natural foci of modern cholera.

A hypothesis on the appearance and persistence of natural foci of cholera based on ecological and bioenergetic features of the process has been developed. The main causes of persistence and propagation of modern cholera are: 1) inability of various bacteria, including the genus Vibrio and many cyanobacterial species, to perform energy coupling, depending on external conditions, by means of two cycles (the proton and sodium cycles); induction of the sodium cycle of energy coupling increases the resistance of bacteria to various environmental factors, such as high concentrations of sodium, alkaline pH, and a high proton conductance of coupling membranes [1], and probably the virulence of the vibrios; 2) development of cyanobacteria in an aquatic environment enriched with Na+ accelerates alkalization of the medium and stimulates the development of the community of cyanobacteria with Vibrio cholerae, an autochthonous inhabitant of saline water bodies and marine shallow waters; 3) salinization of water bodies accelerates their blooming and enriches them with soluble organic matter, a substrate for vibrios inhabiting the biotope; 4) further propagation of cholera infection is related to eating heat-untreated hydrobionts from blooming water bodies [2].

Paroxysmal afterpotentials and role of calcium-dependent potassium conductivity in neuronal activity of strychninized neocortex.

Reactions of cortical suprasylvian gyrus neurons were investigated intracellularly after supracortical strychnine application in immobilized and anaesthetized cats. It was shown that paroxysmal depolarizing shifts of membrane potential could be accompanied by de- and hyperpolarizing afterpotentials. When passing from epileptiform to normal physiological activity, short afterhyperpolarizations, 300-500 ms in duration, were converted into inhibitory postsynaptic potentials which were also accompanied by a decrease in membrane potential.