Simple and rapid assessment of lipoprotein susceptibility to oxidation in the macromolecule fraction of plasma.

Background: Susceptibility of lipoprotein to oxidation is usually studied using purified lipoproteins. However, for large clinical studies or routine clinical assessment, a rapid, less time-consuming method that minimizes hydroperoxide accumulation in lipoprotein during preparation is desirable. Methods using whole plasma are complicated by the presence of an anticoagulant that may affect oxidation and the presence of antioxidants in the plasma aqueous phase.

Lipoprotein susceptibility to oxidation in the macromolecular fraction of plasma: relation to plasma lipids and oleate/linoleate ratio in comparison with whole plasma in hypertriglyceridemia.

Background: Susceptibility of lipoprotein to oxidation is usually studied using purified lipoproteins. However, for large clinical studies or routine clinical assessment, a rapid less time-consuming method is desirable. Therefore, we studied copper-mediated oxidation of the macromolecule fraction of plasma in comparison with oxidation directly in whole unfractionated plasma in a group of hyperlipidemic individuals.