Genetically encoded barcodes for correlative volume electron microscopy.

While genetically encoded reporters are common for fluorescence microscopy, equivalent multiplexable gene reporters for electron microscopy (EM) are still scarce. Here, by installing a variable number of fixation-stable metal-interacting moieties in the lumen of encapsulin nanocompartments of different sizes, we developed a suite of spherically symmetric and concentric barcodes (EMcapsulins) that are readable by standard EM techniques. Six classes of EMcapsulins could be automatically segmented and differentiated.

A place learning assay for tethered walking Drosophila.

Background: Drosophila shows a range of visually guided memory and learning behaviors, including place learning. Investigating the dynamics of neural circuits underlying such behaviors requires learning assays in tethered animals, compatible with in vivo imaging experiments.

New Method: Here, we introduce an assay for place learning for tethered walking flies.

Axial motion estimation and correction for simultaneous multi-plane two-photon calcium imaging.

Two-photon imaging in behaving animals is typically accompanied by brain motion. For functional imaging experiments, for example with genetically encoded calcium indicators, such brain motion induces changes in fluorescence intensity. These motion-related intensity changes or motion artifacts can typically not be separated from neural activity-induced signals.