Quantitative description of the phase-separation behavior of the multivalent SLP65-CIN85 complex.

Biomolecular condensates play a major role in cell compartmentalization, besides membrane-enclosed organelles. The multivalent SLP65 and CIN85 proteins are proximal B-cell antigen receptor (BCR) signal effectors and critical for proper immune responses. In association with intracellular vesicles, the two effector proteins form phase separated condensates prior to antigen stimulation, thereby preparing B lymphocytes for rapid and effective activation upon BCR ligation.

Efficient Access to Elusive 1D C NMR Spectra through Highly Resolved H, C-Long-Range Correlation Spectroscopy.

A method for obtaining 1D C NMR spectra from natural products or metabolites using proton detection is described. The approach delivers singlets for every C signal without conducting any broadband H decoupling (CPD) and is based on calculating C projections from constant-time HMBC and conventional HSQC experiments, recorded at high digital resolution and processed to pure phases. Paramount to the proposed method is the implication of nonuniform sampling and echo processing.

Expanding the Limits of Structural Characterization of Marine Dissolved Organic Matter Using Nonuniform Sampling Frequency-Reversed Edited HSQC NMR.

The multiplicity-edited heteronuclear single quantum correlation (ME-HSQC) NMR method is widely used for the structural characterization of marine dissolved organic matter (DOM), which is a complex molecular mixture comprising millions of individual compounds. However, the standard ME-HSQC suffers from significant signal cancellation and subsequent loss of crucial structural information due to the overlap between CH/CH (positive) and CH (negative) cross-peaks in overcrowded regions. This study introduces nonuniform sampling in frequency-reversed ME-HSQC (NUS FR-ME-HSQC), highlighting its remarkable potential for the comprehensive structural characterization of marine DOM.

Antimicrobial Diterpene Alkaloids from an Sponge Collected in the Yucatán Peninsula.

Three new diterpene alkaloids, (+)-8-epiagelasine T (), (+)-10-epiagelasine B (), and (+)-12-hydroxyagelasidine C (), along with three known compounds, (+)--agelasine F (), (+)-agelasine B (), and (+)-agelasidine C (), were isolated from the sponge , collected on the coasts of the Yucatán Peninsula (Mexico). Their chemical structures were elucidated by 1D and 2D NMR spectroscopy, HRESIMS techniques, and a comparison with literature data. Although the synthesis of (+)--agelasine F () has been previously reported, this is the first time that it was isolated as a natural product.