ACTB::ZMIZ2-rearranged adnexal carcinoma: a second case.

A case of cutaneous adnexal neoplasm with unusual squamoid morphology and harboring an in frame ACTB::ZMIZ2 fusion transcript was recently described. Herein, we report a second case of adnexal carcinoma harboring similar morphology and an identical in frame ACTB::ZMIZ2 fusion transcript. This 2.

Cushing's syndrome related to higher rates and earlier onset of cataract: A nationwide retrospective cohort study.

Purpose: To investigate the risk of cataract in patients with Cushing's syndrome (CS) and evaluate whether disease onset occurs at an earlier age compared to general population.

Methods: A nationwide retrospective matched-cohort study including individuals diagnosed with endogenous CS from 2000 to 2023. Patients with CS were matched in a 1:5 ratio with a control group individually matched for age, sex, socioeconomic status, and body mass index.

Effect of intraoperative magnesium sulphate on acute kidney injury following robot-assisted radical prostatectomy: a retrospective propensity score-matched analysis.

Although intraoperative magnesium sulphate administration has various advantages, its influence on the occurrence of postoperative acute kidney injury (AKI) remains unclear, particularly in patients undergoing robot-assisted radical prostatectomy (RARP). The steep Trendelenburg position and a high intra-abdominal pressure can render patients susceptible to AKI after surgery. This study aimed to evaluate the effects of intraoperative magnesium sulphate administration on postoperative AKI in patients who underwent RARP.

Early and multiple doses of zoledronate mitigates rebound bone loss following withdrawal of RANKL inhibition.

Rebound bone loss following denosumab discontinuation is an important barrier in the effective long-term treatment of skeletal disorders. This is driven by increased osteoclastic bone resorption following the offset of RANKL inhibition, and sequential osteoclast-directed therapy has been utilised to mitigate this. However, current sequential treatment strategies intervene following the offset of RANKL inhibition and this approach fails to consistently prevent bone loss.

Incidence and Presentation of Periprosthetic Joint Infection After Primary Metacarpophalangeal and Proximal Interphalangeal Arthroplasty.

Purpose: Periprosthetic joint infection (PJI) is a devastating complication that has been extensively investigated in large joint arthroplasty. However, this has been inconsistently reported after metacarpophalangeal (MCP) and proximal interphalangeal (PIP) arthroplasty. The objective of the study was to report the presentation and treatment of patients with PJI after MCP or PIP joint arthroplasty.

Screening analysis of doping agents in horse urine and plasma with dilute and shoot using liquid chromatography high resolution mass spectrometry.

Various technical methodologies are required to accurately detect substances of different chemical and pharmacological properties in biological samples, which are increasing in number and variety daily. Therefore, laboratories where many samples and different factors are analyzed simultaneously need methods with easy sample preparation, short analysis times and low analysis costs. In this study, the objective was to scan substances susceptible to chemical degradation, amenable to analysis without hydrolysis, and exhibiting short-term stability by employing a straightforward, expeditious, and cost-efficient method.

Protective association between dietary phytosterol intake and cardiovascular health: an analysis of the UK Biobank cohort.

: Cardiovascular diseases (CVDs) remain a leading cause of morbidity and mortality worldwide, with dietary interventions showing promise in reducing CVD risk factors. Phytosterols (PSs) in plant-based foods may reduce CVD risk by lowering low-density lipoprotein cholesterol. However, the relationship between dietary PS intake and CVD outcomes remains inconclusive.

Quantitative profiling and mapping of small molecules by laser desorption/ionization mass spectrometry: combinations of carbon-based nano-matrices and sample preparation protocols.

The choices of matrices and protocols for sample deposition are critical factors, which impact each other in the matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI). Previous reports on MALDI MS matrices have only compared their performances in terms of their MS signal intensities and provided optical microphotos or MALDI MS images of sample spots but typically lacked quantitative evaluation. Therefore, there is an urgent need to develop a multivariate model to evaluate the performance of different combinations of matrices and sample protocols.

An In Vivo Biostability Evaluation of ALD and Parylene-ALD Multilayers as Micro-Packaging Solutions for Small Single-Chip Implants.

Miniaturization of next-generation active neural implants requires novel micro-packaging solutions that can maintain their long-term coating performance in the body. This work presents two thin-film coatings and evaluates their biostability and in vivo performance over a 7-month animal study. To evaluate the coatings on representative surfaces, two silicon microchips with different surface microtopography are used.

tDDA: A Targeted Data-Dependent Acquisition Mode for Rapid Screening of Targets in Complex Matrices.

Miniaturized mass spectrometers offer significant potential for in situ analysis due to their high specificity and portability. In traditional data-dependent acquisition (DDA) mode, precursor ions for tandem analysis are selected based on the full-scan mass spectrum. However, in situ applications often require the direct analysis of complex samples without extensive sample pretreatment, making them susceptible to chemical noise that can result in false negatives.

Intact N-glycopeptide analysis of human platelets reveals a Glycostructure important for platelet function.

Glycosylation is an important posttranslational modification in platelets, and the glycosylation pattern is critical for platelet function. To date, the exploration of the roles of various glycoforms in specific platelet functions is largely lacking. In this study, a global analysis of intact N-glycopeptides in human platelets was performed to map all the glycopeptides, glycosites and glycans of platelets.

A proteogenomic analysis of the adiposity colorectal cancer relationship identifies GREM1 as a probable mediator.

Background: Adiposity is an established risk factor for colorectal cancer (CRC). The pathways underlying this relationship, and specifically the role of circulating proteins, are unclear.

Methods: Utilizing two-sample univariable Mendelian randomization (UVMR), multivariable Mendelian randomization (MVMR), and colocalization, based on summary data from large sex-combined and sex-specific genetic studies, we estimated the univariable associations between: (i) body mass index (BMI) and waist-hip ratio (WHR) and overall and site-specific (colon, proximal colon, distal colon, and rectal) CRC risk, (ii) BMI and WHR and circulating proteins, and (iii) adiposity-associated circulating proteins and CRC risk.

Evaluation of long-read sequencing for Ostreid herpesvirus type 1 genome characterization from infected tissues.

Since the 1990s, the Pacific oyster has faced significant mortality, which has been associated with the detection of the Ostreid Herpesvirus type 1 (OsHV-1). Due to the complex genomic architecture and the presence of multiple genomic isomers, short-read sequencing using Illumina method struggles to accurately assemble tandem and repeat regions and to identify and characterize large structural variations in the OsHV-1 genome. Third-generation sequencing technologies, as long-read real-time nanopore sequencing from Oxford Nanopore Technologies (ONT), offer new possibilities for OsHV-1 whole-genome analysis.

Physico-chemical properties and substrate specificity of α-(1→3)-d-glucan degrading recombinant mutanase from expressed in .

Unlabelled: The gene encoding fungus mutanase (MutA, GH71 family, α-1,3-glucanase, EC 3.2.1.

Etiological Insights and the Role of Individual Factors in Infectious Spondylodiscitis.

Spondylodiscitis can be caused by various microorganisms and has shown a continuous rise in incidence and mortality. The purpose of our study was to analyze the demographic and laboratory data, as well as comorbidities of patients that were surgically treated for spondylodiscitis in our hospital. The causative pathogens involved in the etiology of spinal infections were also assessed.

Development and Validation of a High-Throughput Quantification Method of Crown Procyanidins in Different Wines by UHPLC-Q-TOF.

Procyanidins are widely distributed in plant-derived foods, and consist of flavanol oligomers and polymers. Recently, the crown procyanidin sub-family, characterised by a unique macrocyclic structure, has been identified in grapes and wine. This study reports the development and validation of a rapid and quantitative analytical method measuring crown procyanidin concentration in red and white wines using ultra-high-performance liquid chromatography (UHPLC) coupled with a Q-TOF mass spectrometer.

Effect of Different Reduced Training Frequencies After 12 Weeks of Concurrent Ballistic and Aerobic Training on Muscle Power and Triceps Brachii Muscle Architecture.

The aim of the present study was to investigate the effect of two long-term reduced concurrent training frequencies (incorporating power training for the upper and high-intensity interval aerobic training for the lower extremities), in which participants performed one training session every either 7 or 14 days, after 12 weeks of systematic concurrent training on upper extremities' muscle strength, power, and morphology in young females. After a 12-week concurrent resistance and aerobic training period, participants were assigned into three groups and performed either one training session every 7 days (G7), or once every 14 days (G14), or detraining (GD) for 12 weeks, followed by 12 additional weeks of detraining. Performance and muscle mass increased after the initial 12-week training period.

The Impact of Rest Intervals on the Force-Velocity Relationship Obtained During the Keiser's 10-Repetition Leg Press Test.

The Keiser 10-rep leg press test protocol employs short inter-repetition rest intervals (2-38 s), raising concerns as to whether athletes perform optimally. The aim of this study was to compare the results of the standard Keiser protocol with an identical protocol modified to include a significantly longer inter-repetition rest intervals and to evaluate whether these effects differed between men and women. A total of 30 athletes (age 17.

Subgroup Characteristics of Middle-Aged and Older Women with Chronic Low Back Pain by Multiple Factors: A Hierarchical Cluster Analysis.

Background/objectives: Chronic low back pain (CLBP) after middle age is a complex multifactorial condition, and subgrouping is recommended to determine effective treatment strategies. Multidimensional data help create new groupings to increase the effectiveness of interventions in middle-aged and older adults with CLBP. This study aimed to investigate the relationship between the factors associated with CLBP after middle age and to create and characterize a new subgroup based on these factors.

Reversing Decline in Aging Muscles: Expected Trends, Impacts and Remedies.

: Age-related decline in musculoskeletal function is a significant concern, particularly in Western countries facing demographic shifts and increased healthcare demands. This review examines the typical trajectories of musculoskeletal deterioration with age and evaluates the effectiveness of various interventions in preventing or reversing these changes. : The review analyzes documented rates of decline across multiple parameters, including muscle mass, Type II muscle fiber reduction, and decreased motor unit firing rates.

Bone Adaptations to a Whole Body Vibration Protocol in Murine Models of Different Ages: A Preliminary Study on Structural Changes and Biomarker Evaluation.

: Whole body vibration (WBV) is a valuable tool to mitigate physiological adaptations related to age and inactivity. Although significant benefits have been found at the musculoskeletal level, including increased bone mass and reduced muscle atrophy, the underlying biological mechanisms remain largely unknown. Therefore, our study aimed to evaluate the effects of vibratory training on bone tissue in murine models of different age groups by investigating the structural and distribution changes in some crucial biomarkers involved in musculoskeletal homeostasis.

Evaluation of Optimized Lumbar Oblique X-Ray Angles with Positioning Assistance for Enhanced Imaging Quality: A Pilot Study in an Asian Cohort.

Pars fractures are a common cause of lower back pain, especially among young individuals. Although computed tomography (CT) and magnetic resonance imaging (MRI) scanning are commonly used in developed regions, traditional radiography remains the main diagnostic method in many developing countries. This study assessed whether the standard radiographic angles suggested in textbooks are optimal for an Asian population since Asian groups have lower lumbar lordosis.

Exploring the Interplay of Handgrip Neuromuscular, Morphological, and Psychological Characteristics in Tactical Athletes and General Population: Gender- and Occupation-Based Specific Patterns.

Background/objectives: The correlation of handgrip strength (HGS) and morphological characteristics with Big Five personality traits is well documented. However, it is unclear whether these relationships also exist in highly trained and specialized populations, such as tactical athletes, and whether there are specific differences compared to the general population. This study aimed to explore the interplay of handgrip neuromuscular, morphological, and psychological characteristics in tactical athletes and the general population of both genders.

Key Performance Indicators for College American Football Starters: An Exploratory Study.

Objectives: The purpose of this study was twofold: (a) to profile body composition and physical fitness characteristics of collegiate American football starters and (b) to examine differences in key performance indicators across position groups. These indicators included select measures of body composition, joint kinematics, as well as muscular strength and power.

Methods: Sixteen National Collegiate Athletic Association (NCAA) Division-II American football athletes (age: 22.