Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations.

For about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development. Its appeal lies in its simple handling and highly versatile application for a wide range of cell types-from bacteria to mammalian cells. In recent decades, extensive research has been conducted on the shake flask, to not perform processes blindly but to gain a deeper understanding of the various process parameters, phenomena, and their impact on the process.

Current status and future directions of application of urine proteomics in neonatology.

With continuous advancements in mass spectrometry technology, researchers increasingly utilize this method to investigate the molecular mechanisms underlying various diseases, and to identify novel diagnostic and therapeutic strategies. Among proteomics applications, urinary proteomics stands out for its non-invasive nature, making it particularly suitable for vulnerable populations like neonates. This review provides a comprehensive overview of recent research on urinary proteomics in the field of neonatology.

Relationship Between Subjectively Assessed Physical Activity (GPAQ) and Quality of Life (WHOQOL-BREF) of Aging Employees in JIPMER, Puducherry: A Cross-Sectional Analytical Study.

Background: Physical activity appears to be one of the most important strategies for preventing physical and mental disorders. Regular physical activity (PA) may prolong life and appears to be a preventative measure against genetic and molecular aging.

Aim: To find the relationship between aging adults' quality of life (QOL) and PA.

Kinematic and muscle co-activation patterns in the dominant arm across forehand stroke phases in wheelchair tennis.

Objective: This study investigated upper limb kinematics and muscle co-activation in wheelchair tennis players during the forehand stroke. By analyzing linear and angular kinematic variables alongside muscle co-activation patterns, the study aimed to provide insights into the biomechanical mechanisms supporting forehand stroke performance.

Method: Fifteen professional male wheelchair tennis players (height: 163.

Isolation and characterization of a β-galactosidase from for industrial processing.

In this study, a thermostable β-galactosidase from OSU-PECh-4A has been isolated through diafiltration and size-exclusion chromatography. The enzyme consists of a heterodimer with a molecular mass of 110 kDa, with a small and large subunit of 36 and 74 kDa, respectively. The Michaelis constant (K) and maximum velocity (V) values for lactose and -nitrophenyl-β-d-galactopyranoside (NPG) hydrolysis were, respectively, 29.

The use of ultrasonography in the transition period to estimate adipose tissue depots and their association with risk of early postpartum hyperketonemia in Holstein dairy cattle.

An elevated prepartum BCS is a risk factor for postpartum hyperketonemia (HYK) and elevated nonesterified fatty acid (NEFA) concentrations in dairy cattle. The association between different adipose tissue depots, such as subcutaneous (SCAT) as well as visceral adipose tissue (VAT) depots, and HYK and elevated NEFA concentrations remains unknown. The objective of this 2-part study was to describe SCAT and VAT depots using ultrasonography during the transition period and to associate them with metabolic markers of negative energy balance in early postpartum dairy cows.

Achieving myoblast engraftment into intact skeletal muscle via extracellular matrix.

Cell therapy of skeletal muscles is a promising approach for the prevention of muscular diseases and age-related muscle atrophy. However, cell transplantation to treat muscle atrophy that does not involve disease, such as sarcopenia, is considered impossible because externally injected cells rarely engraft into non-injured muscle tissue. Additionally, skeletal muscle-specific somatic stem cells, called satellite cells, lose their ability to adhere to tissue after being cultured and transforming into myoblasts.

Media coverage of internally displaced persons in two selected newspapers in Nigeria.

The study focused on the coverage of Internally Displaced Persons (IDPs) in two selected newspapers. It aims to understand the extent and nature of the coverage of IDPs in these newspapers, taking into consideration the various reasons for displacement, such as conflicts and disasters, religious or political persecution, and economic necessity. Through an analysis of the selected newspapers, the study aims to shed light on the portrayal of IDPs, providing a comprehensive understanding of the issue and potential areas for improvement in media coverage.

Evaluation of antihistamine-refractory chronic urticaria patients who used biological agent treatment in terms of cardiovascular risk.

Introduction: The idea that chronic inflammatory processes may play a role in the etiopathogenesis of both treatment - refractory chronic spontaneous urticaria and cardiovascular diseases is an important research topic.

Aim: Within the scope of this research, we aimed to elucidate a new perspective on the follow-up of chronic urticaria patients by evaluating the 10-year cardiovascular risk and metabolic syndrome in resistant chronic spontaneous urticaria patients who were unresponsive to maximum antihistamine treatment.

Material And Methods: A total of 170 individuals who applied to our institution's Health Science University, Tepecik Education and Research Hospital, allergy and immunology outpatient clinic have been analysed in this retrospective case-control study.

Development and application of a non-targeted analysis method using GC-MS and LC-MS for identifying chemical contaminants in drinking water via point-of-use filters.

While many chemicals are regulated and routinely monitored in drinking water, they represent just a portion of all contaminants that may be present. Typical drinking water analyses involve sampling one liter or less of water, which could lead to trace level contaminants going undetected. In this study, a method was developed for using point-of-use activated carbon block drinking water filters as sampling devices.

Difficulties in the diagnosis and treatment of axillary malignant triton tumors: A case report.

Malignant triton tumor (MTT), a subtype of malignant peripheral nerve sheath tumor, is a rare soft-tissue sarcoma with a difficult diagnosis and poor prognosis. The course of MTT progression is rapid and the degree of malignancy is high. Patients with MTT can be treated with postoperative adjuvant radiotherapy and chemotherapy; however, treatment results are still poor.

Spontaneous Regression of Locally Advanced Breast Cancer Following Cardiopulmonary Arrest: A Case Report.

Spontaneous regression in breast cancer is rare but can dramatically improve patient prognosis. Although the underlying mechanism is unknown, it may be due to a biological response to external invasion. An 81-year-old woman presented to our emergency department with a 600x100mm large breast mass.

Physiological effects of hand grip and cold pressor tests in young Saudi adults.

The risk of cardiovascular disease differs among various ethnic groups, highlighting disparities in cardiovascular health among different populations. While multiple studies from other countries have looked at changes in physiological parameters during autonomic function tests like isometric handgrip and cold pressor tests, no correlational research has been done in Saudi Arabia. This lacuna underscores the importance of examining the relationship between cardiorespiratory parameters in young Saudi Arabian individuals during these tests.

Bacteriocin from the Raccoon Dog Oral Microbiota Inhibits the Growth of Pathogenic Methicillin-Resistant Staphylococcus aureus.

The growing incidence of infections caused by antibiotic-resistant strains of pathogens is one of the key challenges of the 21 century. The development of novel technological platforms based on single-cell analysis of antibacterial activity at the whole-microbiome level enables the transition to massive screening of antimicrobial agents with various mechanisms of action. The microbiome of wild animals remains largely underinvestigated.

The Characteristics of the Metabolomic Profile in Patients with Parkinson's Disease and Vascular Parkinsonism.

The gradually increasing age of the world population implies that the prevalence of neurodegenerative diseases also continues to rise. These diseases are characterized by a progressive loss of cognitive and motor functions. Parkinson's disease, which involves the gradual death of specialized neural tissue, is a striking example of a neurodegenerative process.

Fertility induction associated with improved peripheral reproductive parameters in male mice subjected to GH and levothyroxine replacement.

Objective: The aim of this study was to characterize the parameters of reproductive anatomy and pituitary hormone expression levels in ames dwarf mice ).

Materials And Methods: Male mice aged 30 days received daily intraperitoneal injections of recombinant human GH and levothyroxine three times weekly for 60 days. The sexual maturation of these animals was compared with that of their wild-type ( ) and untreated ( ) siblings.

The hypothalamus as the central regulator of energy balance and its impact on current and future obesity treatments.

The hypothalamus is a master regulator of energy balance in the body. First-order hypothalamic neurons localized in the arcuate nucleus sense systemic signals that indicate the energy stores in the body. Through distinct projections, arcuate nucleus neurons communicate with second-order neurons, which are mostly localized in the paraventricular nucleus and in the lateral hypothalamus.

Association between the aromatase () gene variant rs10046 and cardiovascular risk in postmenopausal women.

Objective: To assess the genotypic and allelic distribution of the rs10046 polymorphism in the gene and evaluate whether this aromatase gene variant is associated with cardiovascular risk in postmenopausal women.

Materials And Methods: This cross-sectional study analyzed repository-stored samples from 370 postmenopausal women aged 44-72 years. Clinical, metabolic, and hormonal data were collected.

A Rare Case of Facial Arteriovenous Malformation in a 3-Year-Old Female.

Arteriovenous malformation (AVM) is a rare congenital vascular anomaly involving abnormal artery-vein connections that bypass the capillary system. AVMs are particularly uncommon in young children. A 3-year-old girl presented with a painless, progressively enlarging left cheek swelling since birth.

Construction of an MXene/MIL Fe-53/ZIF-67 derived bifunctional electrocatalyst for efficient overall water splitting.

Research on water splitting is paramount for developing low-carbon alternative energy sources. Nevertheless, creating an efficient, cost-effective, and bifunctional electrocatalyst that facilitates both the oxygen evolution reaction (OER) and the hydrogen evolution reaction (HER) remains an elusive goal. In this work, we report a novel hybrid nanostructured electrocatalyst by combining and pyrolyzing MXene, MIL-53(Fe), and ZIF-67.

Investigating the structure-property correlations of pyrolyzed phenolic resin as a function of degree of carbonization.

Carbon-carbon (C/C) composites are attractive materials for high-speed flights and terrestrial atmospheric reentry applications due to their insulating thermal properties, thermal resistance, and high strength-to-weight ratio. It is important to understand the evolving structure-property correlations in these materials during pyrolysis, but the extreme laboratory conditions required to produce C/C composites make it difficult to quantify the properties . This work presents an atomistic modeling methodology to pyrolyze a crosslinked phenolic resin network and track the evolving thermomechanical properties of the skeletal matrix during simulated pyrolysis.

The correlation between insulin resistance and blood lipids in children.

Background: This work focused on the correlation between insulin resistance (IR) and blood lipids (BL) in children with simple obesity, as well as the intervention effects of high-intensity interval training (HIIT) in weight loss in children.

Methods: 80 children aged 6 to 17 years with simple obesity were selected from our hospital and randomly grouped into two groups. Children in the control (Ctrl) group underwent traditional moderate-intensity continuous training (MICT), while those in the other group received HIIT (HIIT group).

Perceived Stress During Late Pregnancy and Infant Body Composition at 1 Month.

Context: Worldwide, obesity remains one of the most challenging crises with children being one of the most susceptible populations. The effect of maternal stress during pregnancy on newborn body composition, measured by fat mass and lean mass has, not been extensively studied.

Objectives: We evaluated the association between perceived stress during late pregnancy and infant adiposity at 1 month and assessed effect modification by infant sex and preterm birth.

Comparison of flavor components between normal and gas-producing wasabi based on HS-GC-IMS, HS-GC-MS and electronic sensory technology.

Wasabi is a type of sauce made from the plant horseradish. During its production and storage, gas production sometimes occurs, which leads to changes in the flavor quality of wasabi. In this study, an electronic nose, electronic tongue, headspace-gas chromatography-mass spectrometry and headspace-gas chromatography-ion mobility spectrometry combined with multivariate statistical analysis were used to compare the differences in odor, taste and volatile components between normal and gas-producing wasabi.

Be Aware of Transient Dissolution Processes in CoO Acidic Oxygen Evolution Reaction Electrocatalysts.

Recently, cobalt-based oxides have received considerable attention as an alternative to expensive and scarce iridium for catalyzing the oxygen evolution reaction (OER) under acidic conditions. Although the reported materials demonstrate promising durability, they are not entirely intact, calling for fundamental research efforts to understand the processes governing the degradation of such catalysts. To this end, this work studies the dissolution mechanism of a model CoO porous catalyst under different electrochemical conditions using online inductively coupled plasma mass spectrometry (online ICP-MS), identical location scanning transmission electron microscopy (IL-STEM), and differential electrochemical mass spectrometry (DEMS).