Bone-derived nanoparticles (BNPs) enhance osteogenic differentiation Notch signaling.

Mesenchymal stem cell (MSC)-based bone tissue regeneration has gained significant attention due to the excellent differentiation capacity and immunomodulatory activity of MSCs. Enhancing osteogenesis regulation is crucial for improving the therapeutic efficacy of MSC-based regeneration. By utilizing the regenerative capacity of bone ECM and the functionality of nanoparticles, we recently engineered bone-based nanoparticles (BNPs) from decellularized porcine bones.

Antagonisation of Prokineticin Receptor-2 Attenuates Preeclampsia Symptoms.

Preeclampsia (PE) is the most threatening pathology of human pregnancy. Placenta from PE patients releases harmful factors that contribute to the exacerbation of the disease. Among these factors is the prokineticin1 (PROK1) and its receptor, PROKR2 that we identified as a mediators of PE.

Blood cortisol and faecal cortisol metabolite concentrations following an ACTH challenge in unanaesthetized brown bears ().

Faecal cortisol metabolites (FCMs) are increasingly used to index physiological stress in wildlife. Cortisol and other stress hormones act to mobilize glucose, providing energy for the organism to respond to environmental perturbations. Cortisol, the predominant glucocorticoid (GC) in most mammals, is metabolized by the liver and excreted as FCMs.

Promoting Angiogenesis/Osteogenesis by a New Copper/Magnesium Hydroxide Hybrid Nanoparticle: In Vitro and In Vivo Investigation.

In this study, a new hybrid nanoparticle composed of magnesium hydroxide and copper oxide (Mg(OH)/CuO) with an optimized ratio of magnesium (Mg) to copper (Cu) was designed and incorporated into a 3D-printed scaffold made of polycaprolactone (PCL) and gelatin. These hybrid nanostructures (MCNs) were prepared using a green, solvent-free method. Their topography, surface morphology, and structural properties were characterized using scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS).

The Albright-Losee Low Profile Pivot Shift Test for ACL Deficiency.

Background: Recognizing ACL injuries on the field and in the office can be very challenging in awake and apprehensive patients. Despite high specificity, many published "pivot-shift" techniques have limited acceptance mainly because of unsatisfactory sensitivity. We describe in detail, four specific modifications and provide a critical review of our clinical experiences to empower the new user's readiness to master a novel screening procedure for ACL disruption.

Recommendations for Specialty Palliative APRN Graduate Education.

Background: Advanced practice registered nurses (APRNs) are essential to care for the growing number of individuals with advanced illness given the shortage of palliative care clinicians.

Problem: Graduate education for specialty practice palliative APRNs lacks consistency in structure, content, and standardization of specialty palliative APRN education.

Approach: A workgroup of expert palliative APRNs and graduate faculty conducted focused discussions and a literature review to develop consensus recommendations based on national palliative APRN competencies and aligned with the 8 core concepts of the AACN Essentials.

Rapid implementation of open-access pandemic education for global frontline healthcare workers.

Background: The recent global pandemic posed extraordinary challenges for healthcare systems. Frontline healthcare workers required focused, immediate, practical, evidence-based instruction on optimal patient care modalities as knowledge evolved around disease management.

Objective: This course was designed to provide knowledge to protect healthcare workers; combat disease spread; and improve patient outcomes.

Differential associations among psychological flexibility components, depressive symptoms, and sense of belonging in first semester college students.

The transition to college is associated with rising rates of depressive symptoms and decreased well-being. It is critical to identify protective psychological factors for this period. One possible protective factor is psychological flexibility, or the ability to pursue self-identified values despite distressing thoughts and emotions.

Smartphone based non invasive real time white blood cell counter leveraging blue light and static magnetic field.

White blood cells (WBCs), also known as leukocytes, are one of the most significant parts of the immune system. They generate antibodies, protect the body from illnesses, and heal wounds. Accurate estimation of WBCs is key for diagnosing cancer, infections, leukemia, lymphoma, and other diseases.

Evaluating the Efficacy of and Preference for Interactive Computer Training with Student-Generated Examples.

Designing effective and preferred teaching practices for undergraduate students are common goals in behavior analytic training programs. A preliminary study by Nava et al. (2019) showed that undergraduate students generally rated peer-generated examples of the principles of behavior analysis as more preferred, relatable, and culturally responsive than traditional textbook examples.

One-quarter of freshwater fauna threatened with extinction.

Freshwater ecosystems are highly biodiverse and important for livelihoods and economic development, but are under substantial stress. To date, comprehensive global assessments of extinction risk have not included any speciose groups primarily living in freshwaters. Consequently, data from predominantly terrestrial tetrapods are used to guide environmental policy and conservation prioritization, whereas recent proposals for target setting in freshwaters use abiotic factors.

A generalized objective CT-based method for quantifying articular fracture severity.

A CT-based method for objectively assessing fracture severity was previously developed and validated to address poor reliability in existing subjective fracture classification systems. The method involved quantifying the energy involved in creating a fracture. However, clinical utility of the method was hindered by reliance upon an intact contralateral CT and lengthy analysis time (8-10 h).

Building Health Policy Advocacy Expertise in Pediatric Nurse Practitioners Through Civic-Based Experiential Learning.

Background: Many nurses work largely as policy implementers rather than policy developers. The literature posits several multifaceted reasons for this lack of policy acumen including interprofessional power dynamics, marginalization of nurses in policy making, and lack of formal training in public policy advocacy.

Purpose: To evaluate the impact of a targeted teaching strategy on increasing political astuteness, perceived skill, and comfort in health policy advocacy among a cohort of acute care pediatric nurse practitioner students.

Curcumin and Its Potential to Target the Glycolytic Behavior of Lactate-Acclimated Prostate Carcinoma Cells with Docetaxel.

Dysregulated cellular metabolism is known to be associated with drug resistance in cancer treatment. In this study, we investigated the impact of cellular adaptation to lactic acidosis on intracellular energy metabolism and sensitivity to docetaxel in prostate carcinoma (PC) cells. The effects of curcumin and the role of hexokinase 2 (HK2) in this process were also examined.

Simultaneous Emergent Phenomena: Leadership and Team Synchrony.

Emergent phenomena exhibit interesting dynamics when considered individually. The present article examines two emergent processes that could be occurring simultaneously in an intense team interaction: the emergence of leaders and the emergence of autonomic synchrony within teams making dynamic decisions. In the framework of panarchy theory and related studies on complex systems, autonomic synchrony would be a fast dynamic that is shaped or controlled by leadership emergence, which is a slower dynamic.

Introduction to Emergence in Social Systems.

The articles in this special issue examine the contributions of Jeffrey A. Goldstein to the understanding of emergence as a formal group of processes. Applications include work teams, organizations, ecologies of organizations, and societies.

A valid novel ground reaction force distribution algorithm to determine midfoot kinetics of gait with a single force plate.

Background: Understanding midfoot joint kinetics is valuable for improved treatment of foot pathologies. Segmental foot kinetics cannot currently be obtained in a standard gait lab without the use of multiple force plates or a pedobarographic plate overlaid with a force plate due to the single ground reaction force (GRF) vector.

Research Question: Can an algorithm be created to distribute the GRF into multiple segmental vectors that will allow for calculation of accurate midfoot and ankle moments?

Methods: 20 pediatric subjects (10 typically developing, 10 with foot pathology) underwent multi-segment foot gait analysis using the Milwaukee Foot Model.

Single-nuclei sequencing reveals a robust corticospinal response to nearby axotomy but overall insensitivity to spinal injury.

The ability of neurons to sense and respond to damage is crucial for maintaining homeostasis and facilitating nervous system repair. For some cell types, notably dorsal root ganglia (DRG) and retinal ganglion cells (RGCs), extensive profiling has uncovered a significant transcriptional response to axon injury, which influences survival and regenerative outcomes. In contrast, the injury responses of most supraspinal cell types, which display limited regeneration after spinal damage, remain mostly unknown.

EGFR status assessment using reflex testing targeted next-generation sequencing for resected non-squamous non-small cell lung cancer.

EGFR status assessment is mandatory for adjuvant decision-making of resected stage IB-IIIA non-squamous non-small cell lung cancer (NS-NSCLC). It is questionable whether single-gene RT-PCR versus next-generation sequencing (NGS) should be used for this evaluation. Moreover, co-occurring mutations have an impact on tumor behavior and may influence future therapeutic decision-making.

Impact of a Quality Cardiopulmonary Resuscitation Coach on Pediatric Intensive Care Unit Resuscitation Teams.

Background: The quality cardiopulmonary resuscitation (CPR) coach role was developed for hospital-based resuscitation teams. This supplementary team member (CPR coach) provides real-time, verbal feedback on chest compression quality to compressors during a cardiac arrest.

Objectives: To evaluate the impact of a quality CPR coach training intervention on resuscitation teams, including presence of coaches on teams and physiologic metrics of quality CPR delivery in real compression events.

Perceptions of Sexism Through a Latine Lens.

This study aims to compare Latine men and women's perceptions of hostile and benevolent sexist behaviors, while also examining the associations between these perceptions and Latine cultural values. Participants who identified as Latine (55 men, 57 women; = 32.05, = 10.

Hydrolysis of the acetyl-CoA allosteric activator by Staphylococcus aureus pyruvate carboxylase.

Pyruvate carboxylase (PC) catalyzes the carboxylation of pyruvate to oxaloacetate which serves as an important anaplerotic reaction to replenish citric acid cycle intermediates. In most organisms, the PC-catalyzed reaction is allosterically activated by acetyl-coenzyme A. It has previously been reported that vertebrate PC can catalyze the hydrolysis of acetyl-CoA, offering a potential means for the enzyme to attenuate its allosteric activation.

Locked Dimerized CXCL12 Exerts Radiosensitizing Effects in Head and Neck Cancer.

Background: Head and neck squamous cell carcinoma (HNSCC) presents significant treatment challenges, particularly in cases unrelated to human papillomavirus (HPV). The chemokine receptor CXCR4, interacting with its ligand CXCL12, plays a crucial role in tumor proliferation, metastasis, and treatment resistance. This study explores the therapeutic potential of engineered monomeric and dimerized CXCL12 variants (CXCL12 and CXCL12, respectively) in HNSCC and evaluates potential additive effects when combined with radiation therapy.