Design of solid lipid nanoparticles for skin photoprotection through the topical delivery of caffeic acid-phthalimide.

Exposure of the skin to ultraviolet (UV) radiation is associated with many pathological conditions such as premature aging and skin cancer. Furthermore, members of Nicotinamide Adenine Dinucleotide Phosphate-oxidase (NADPH oxidase or NOX) enzyme family can produce UV-induced reactive oxygen species (ROS), even after cessation of radiation exposure. The caffeic acid-phthalimide (CF) compound is a potent antioxidant, which reduces the generation of ROS.

Allelic variability in the Rpp1 locus conferring resistance to Asian soybean rust revealed by genome-wide association.

Soybean is a crucial crop for the Brazilian economy, but it faces challenges from the biotrophic fungus Phakopsora pachyrhizi, which causes Asian Soybean Rust (ASR). In this study, we aimed to identify SNPs associated with resistance within the Rpp1 locus, which is effective against Brazilian ASR populations. We employed GWAS and re-sequencing analyzes to pinpoint SNP markers capable of differentiating between soybean accessions harboring the Rpp1, Rpp1-b and other alternative alleles in the Rpp1 locus and from susceptible soybean cultivars.

High carbohydrate is preferable to high lipid parenteral nutrition in healthy dogs undergoing prolonged sedation.

Parenteral nutrition (PN) is commonly used in intensive care units (ICUs) and is associated with earlier hospital outcome. However, there is scarcity of information about the metabolic effects of PN caloric distribution for dogs. Considering the high tolerance of dogs to lipids and, also, that hospitalized animals usually present insulin resistance, PN formulation with high fat instead high glucose can provide metabolic benefits in this specie.

Ultrasonication and thermosonication applied in the processing of jalapeno pepper ( var. ) sauce.

Ultrasonication is one of the non-thermal physical methods that can be used on foods and when used in synergy with temperature (thermosonication), this technique proves to be more effective, thus reducing the duration and intensity of heat treatment and the consequent damage to the foods. This work aimed to use the technique of ultrasonication and thermosonication in the processing of jalapeno pepper sauces in comparison with pasteurization. Two types of sauces were produced, one with pre-cooking (a) and the other without cooking (b), and the influence of time and temperature was analyzed by applying ultrasonication and thermosonication.

Thermal and non-thermal treatments in the processing of cagaita nectar - .

This work aimed to analyze cagaita nectar subjected to different thermal and non-thermal treatments regarding its quality over 30 days of storage (5 °C). Ultra (U) and thermosonication (T) were the techniques used for 30 and 60 minutes of processing samples. These techniques proved to be effective to preserve physicochemical quality, regarding rheology and texture, since ultra and thermosonicated samples had their consistency increased when compared to the pasteurized sample over 30 days, a desirable factor for a fruit nectar.

Glycine max (L.) Merr. (Soybean) metabolome responses to potassium availability.

Potassium (K) has vital physiological and metabolic functions in plants and its availability can impact tolerance to biotic and abiotic stress conditions. Limited studies have investigated the effect of K fertilization on soybean metabolism. Using integrated omics, ionomics and metabolomics, we investigated the field-grown Glycine max (soybean) response, after four K soil fertilization rates.

Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation.

Objectives: To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation.

Methods: Dentin beams (5 × 2 × 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens.

Citric acid incorporated in a chitosan film as an active packaging material to improve the quality and duration of matured cheese shelf life.

Chitosan-based film incorporated with citric acid was prepared by the casting method for application in a Brazilian matured cheese. Three formulations of cheese were processed, with the intention of evaluating the application of a starter culture and the effect of the film in terms of its physiochemical, microbiological, and sensorial characteristics. It was observed by scanning electron microscopy (sem) analysis that the film has a homogeneous appearance, and the crosslinking between citric acid and chitosan was confirmed by the Fourier transform infrared spectroscopy (FTIR) analysis.

Development of a caffeic acid-phthalimide hybrid compound for NADPH oxidase inhibition.

NADPH oxidases are pharmacological targets for the treatment of inflammation-based diseases. This work presents the synthesis and study of a caffeic acid/phthalimide hybrid compound (C2) as a potential inhibitor of NADPH oxidases. Throughout the study, we have compared compound C2 with its precursor caffeic acid (C1).

Effects of imidacloprid on viability and increase of reactive oxygen and nitrogen species in HepG2 cell line.

Imidacloprid (IMD) is a neonicotinoid insecticide used in large quantities worldwide in both veterinary and agronomic applications. Several studies have shown adverse effects of IMD on non-target organisms, with the liver being identified as the main affected organ. This study aimed to evaluate the effects of IMD on human hepatoblastoma (HepG2) cells.

H, N, and C resonance assignments of the SH3-like tandem domain of human KIN protein.

KIN is a DNA/RNA-binding protein conserved evolutionarily from yeast to humans and expressed ubiquitously in mammals. It is an essential nuclear protein involved in numerous cellular processes, such as DNA replication, class-switch recombination, cell cycle regulation, and response to UV or ionizing radiation-induced DNA damage. The C-terminal region of the human KIN (hKIN) protein is composed of an SH3-like tandem domain, which is crucial for the anti-proliferation effect of the full-length protein.

Spontaneous Behaviors of Post-Orchiectomy Pain in Horses Regardless of the Effects of Time of Day, Anesthesia, and Analgesia.

This prospective and longitudinal study aimed to identify spontaneous post-orchiectomy pain behaviors in horses regardless of the effects of anesthesia, analgesia, and recording time of day. Twenty-four horses divided into four groups were submitted to: inhalation anesthesia only (GA), or combined with previous analgesia (GAA), or orchiectomy under pre (GCA), or postoperative (GC) analgesia. The data obtained from the subtraction of frequency and/or duration of 34 behaviors recorded during seven 60-min time-points in the 24 h after the anesthesia from those recorded in the mirrored time-points in the 24 h before the anesthesia (delta) were compared over time and among groups by Friedman and Kruskal-Wallis tests, respectively ( < 0.

Kopsanone inhibits proliferation and migration of invasive colon cancer cells.

Colorectal cancer (CRC) is the second leading cause of cancer-related death globally. In spite of the increasing knowledge on molecular characteristics of different cancer types including CRC, there is limitation in the development of an effective treatment. The present study aimed to verify the antitumor effect of kopsanone, an indole alkaloid.

Correlation between 2D and 3D measurements of cement space in CAD-CAM crowns.

Statement Of Problem: Although the 2D analysis of prosthesis cementation space has been popular, its correlation with volumetric comparison (3D data) of cement space is unclear.

Purpose: The purpose of this in vitro study was to evaluate the cement space in computer-aided design and computer-aided manufacturing (CAD-CAM) crowns of different materials and correlate 2D measurements of cement space with their corresponding 3D values (volume of cement space) by using microcomputed tomography (μCT) analysis of regions of interest.

Material And Methods: Ten molar crowns were milled in lithium disilicate (LD), resin nanoceramic (RN), and zirconia (Z) ceramics.

2-acetylphenothiazine protects L929 fibroblasts against UVB-induced oxidative damage.

Ultraviolet B (UVB) light corresponds to 5% of ultraviolet radiation. It is more genotoxic and mutagenic than UVA and causes direct and indirect cellular damage through the generation of reactive oxygen species (ROS). Even after radiation, ROS generation may continue through activation of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) enzyme.

Comparative Analysis Using Pulsed-Field Gel Electrophoresis Highlights a Potential Transmission of Between Asymptomatic Buffaloes and Pigs in a Single Farm.

Buffaloes and pigs play an important epidemiological roll in the infection cycle, and asymptomatic animals can act as key component in the dissemination of the disease by horizontal, vertical, and cross-species transmission. Our study aimed and was able to confirm evidences of a cross-species transmission of Agona between asymptomatic buffaloes and pigs. Also, we described infection within the pig production phases, involving serotypes Agona, Senftenberg and Schwarzengrund.

HLA-DPB1 and HLA-C alleles are associated with leprosy in a Brazilian population.

Despite intense efforts, the number of new cases of leprosy has remained significantly high over the past 20 years. Host genetic background is strongly linked to the pathogenesis of this disease, which is caused by Mycobacterium leprae (M. leprae), and there is a consensus that the most significant genetic association with leprosy is attributed to the major histocompatibility complex (MHC).

Association of Exon 1 Polymorphisms With Multibacillary Leprosy.

Mannose-binding lectin (MBL) is a serum protein of innate immunity, with a central role in the activation of the complement system through the lectin pathway. This protein is encoded by gene, and single-nucleotide polymorphisms located at exon 1, such as rs5030737 C>T ( variant), rs1800450 G>A ( variant), and rs1800451 G>A ( variant), may change the MBL structure and the serum concentration. polymorphisms have been associated with several infectious diseases, including leprosy.

Postoperative Analgesic Effects of Laserpuncture and Meloxicam in Bitches Submitted to Ovariohysterectomy.

Background: This prospective, randomised and blind study investigated the efficacy of laserpuncture for postoperative pain management in dogs.

Method: Sixteen bitches were sedated with acepromazine and randomly treated before ovariohysterectomy with meloxicam 0.2 mg·kg intramuscular or laserpuncture (wavelength 904 mm, frequency 124 Hz, potency 10 Joules, 100 s in each acupoint).

Preliminary survey of the attitudes of Brazilian scientists towards pain management and assessment in animals used in science.

Objective: To investigate the current scenario in Brazil regarding pain assessment and control in experimental animals.

Study Design: Prospective survey.

Methods: A confidential questionnaire was available online and sent by e-mail to Brazilian scientists working with animal experimentation in Brazil.

Sodium alginate with turmeric coating for ripened cheeses.

Artisanal cheeses produced with high coliform counts are commonly on the market in several countries. The bioactive edible coating use appears as technological innovation in the dairy derivatives market to improve quality and increasing the products shelf life. The objective of this study was to evaluate the characteristics of cheeses produced with containing high coliform counts and coated with tumeric and sodium alginate.

Enhanced cytotoxicity of imidacloprid by biotransformation in isolated hepatocytes and perfused rat liver.

Imidacloprid (IMD) is a neonicotinoid insecticide widely used in crops, pets, and on farm animals for pest control, which can cause hepatotoxicity in animals and humans. In a previous study using isolated rat liver mitochondria, we observed that IMD inhibited the activity of FF-ATP synthase. The aim of this study was to evaluate the effects of IMD on rat isolated hepatocytes and perfused rat liver, besides the influence of its biotransformation on the toxicological potential.

Analysis of correlation between optical and microtomography measurements of cementation space in CAD-CAM ceramic crowns.

Statement Of Problem: Identical computer-aided design (CAD) parameters may be used to mill a variety of ceramic materials for computer-aided design and computer-aided manufacture (CAD-CAM) crowns. Whether milled crown spacing matches the designed parameters when more than a single evaluation method is applied is unknown.

Purpose: The purpose of this in vitro study was to correlate the stereomicroscope and the microcomputer tomography (μCT) 2-dimensional analysis of the cementation space with the replica technique in assessing 3 different ceramic materials.

An Origin of Citations: Darwin's Collaborators and Their Contributions to the Origin of Species.

In the first edition of the Origin of Species (1859), Charles Darwin apologized for not correctly referencing all the works cited in his magnum opus. More than 150 years later we have catalogued these citations and analyzed the resultant data. Looking for a complete selection of collaborators, a flexible interpretation of the term citation was necessary; we define it as any reference made to a third party, independently of its form or function.