Identification, functional characterization and expression pattern of interferon-gamma (IFN-γ) and interferon-gamma receptor 1 (IFNGR1) in Nibea albiflora.

Interferon-gamma (IFN-γ) is an inflammatory cytokine that plays a crucial role in regulating both innate and cell-mediated immune responses by binding to a receptor complex made up of IFNGR1 and IFNGR2. In this study, the complete cDNA of IFN-γ and IFNGR1 from Nibea albiflora were cloned and functionally characterized (named NaIFN-γ and NaIFNGR1), whose complete cDNA sequences were 1593 bp and 2792 bp, encoding 201 and 399 amino acids, respectively. Multiple sequence alignment and phylogenetic analysis showed that the concluded amino acids sequences of NaIFN-γ and NaIFNGR1 shared high identity with their teleost orthologues including the IFN-γ signature and nuclear localization signal (NLS) motif in NaIFN-γ and FN Ⅲ domain in NaIFNGR1.

Insulin-like growth factor 1 promotes the gonadal development of Pampus argenteus by regulating energy metabolism†.

Insulin-like growth factor 1 (Igf1) is known to promote ovarian maturation by interacting with other hormones. However, the limited research on the role of Igf1 in the energy metabolism supply of gonads has hindered further exploration. To explore the role of Igf1 in gonadal development of silver pomfret, we analyzed the expression levels and the localization of igf1 mRNA and protein during testicular and ovarian development of silver pomfret.

Insights into the Deep Phylogeny and Novel Gene Rearrangement of Mytiloidea from Complete Mitochondrial Genome.

The extant marine mussels which belong to the Mytiloidea are widespread species inhabiting mostly coastal waters, with some distributed in the deep sea. To clarify the classification systems and phylogenetic relationships range from genus to family level within Mytiloidea, new sequence was used in a phylogenetic analysis including all the available Mytiloidea mitochondrial genomes. In this study, the complete mitochondrial genome of Vignadula atrata is 15,624 bp in length and contains 12 protein-coding genes (PCGs, atp8 is absent), two ribosomal RNA genes, and 22 transfer RNA genes.

The Complete Mitochondrial Genome of (Brachyura: Pilumnidae), Novel Gene Rearrangements, and Phylogenetic Relationships of Brachyura.

is a crab that belongs to Pilumnidae, Brachyura. Although many recent studies have focused on the phylogeny of Brachyura, the internal relationships in this clade are far from settled. In this study, the complete mitogenome of was sequenced and annotated for the first time.

Characterization of a novel Toll-like receptor 13 homologue from a marine fish Nibea albiflora, revealing its immunologic function as PRRs.

Congenital immunity mediated by Toll-like receptor (TLR) family is the first line of defense for disease-resistant immunity of fish and plays a vital role as a bridge between innate immunity and acquired immunity. As a less known member of the TLR family TLR13 can participate in the immune and inflammatory reactions of the body for recognizing the conserved sequence of 23S rRNA in bacteria and induce immune response. In this study, the full-length cDNA of TLR13 from Nibea albiflora (named as NaTLR13) was cloned and was functionally characterized.

Expression and cellular localization of insulin-like growth factor 3 in gonads of the seasonal breeding teleost silver pomfret (Pampus argenteus).

Insulin-like growth factor 3 plays an important role in gonad development in teleost fish. Previous studies found that igf3 was specifically expressed in gonads of silver pomfret (Pampus argenteus). Unlike in other fish, IGF3 is a membrane protein in silver pomfret, and its specific role in gonads is unclear.

Insights into the Deep Phylogeny and Novel Divergence Time Estimation of Patellogastropoda from Complete Mitogenomes.

To further understand the origin and evolution of Patellogastropoda, we determined the mitochondrial genome sequence of , and compared its mitogenome characteristics with the other four limpets of Nacellidae. The ratio of Ka and Ks indicated that these Nacellidae species were suffering a purifying selection, with exception of the gene. The gene sequence is basically consistent among families, while there are great differences among Lottidae species.

Sequence comparison of the mitochondrial genomes of five brackish water species of the family Neritidae: Phylogenetic implications and divergence time estimation.

Neritids are ancient gastropod species which can live in marine, brackish water, and freshwater environments. In this study, we sequenced and annotated the mitochondrial genomes of five brackish water neritids (i.e.

Neonicotinoid pollution in marine sediments of the East China Sea.

Neonicotinoid insecticides are widely and exceedingly applied in farmlands worldwide and are ubiquitous in various environments, including surface water, soil, river sediments, etc. However, few studies reported neonicotinoid residues in the marine environment. Considering the large application of neonicotinoids in China, here, we collected marine sediments in offshore and far sea areas of the East China Sea, including the Hangzhou Bay and the area along the Zhejiang Province coast, and measured the concentrations of nine commercialized neonicotinoids.

Sequence and functional features of a novel scavenger receptor homolog, SCARA5 from Yellow drum (Nibea albiflora).

As an important member in SR-As, member 5 (SCARA5) can swallow apoptotic cells and foreign bodies, and participate multiple signaling pathways to inhibit tumor occurrence, development growth and metastasis. To explore its immune function, SCARA5 was identified from the yellow drum (Nibea albiflora) according to its transcriptome data, and its full-length cDNA was 6968 bp (named as NaSCARA5, GenBank accession no: MW070211) encoding 497 amino acids with a calculated molecular weight of 55.12 kDa, which had the typical motifs of SR family, such as transmembrane helix region, coil region, Pfam collagens region and SR region.

Identification, functional characterization and expression pattern of myeloid differentiation factor 88 (MyD88) in Nibea albiflora.

Myeloid differentiation factor 88 (MyD88), composed of an N-terminal death domain and a C-terminal Toll/interleukin (IL)-IR homology domain, is a key connector protein in the TLR signal transduction pathway. In this study a novel isoform of MyD88 in Nibea albiflora (named as NaMyD88) was identified and functionally characterized (GenBank accession no. MN384261.

Molecular insights of a novel fish Toll-like receptor 9 homologue in Nibea albiflora to reveal its function as PRRs.

Toll-like receptors (TLRs) are an important class of molecules involved in non-specific immunity, and they are also the bridge connecting between non-specific immunity and specific immunity. As a vital member of TLR family TLR9 can be activated by bacterial DNA and induce the production of inflammatory cytokines. In this study, a full length of TLR9 homologue of 3677 bp in Nibea albiflora (named as NaTLR9, GenBank accession no: MN125017.

Occurrence and partitioning of bisphenol analogues, triclocarban, and triclosan in seawater and sediment from East China Sea.

Bisphenol analogues (BPs), triclocarban (TCC), and triclosan (TCS) are well-known environmental endocrine disrupters. Many studies have characterized their occurrence in the freshwater environment. However, their environmental behaviors in the coastal marine environment remain poorly understood.

Legacy and emerging poly- and perfluorochemicals in seawater and sediment from East China Sea.

Following the global phase out of perfluorooctane sulfate (PFOS), chlorinated polyfluoroalkyl ether sulfonates (Cl-PFAESs) and p-perfluorous nonenoxybenzenesulfonate (PFNOBS) have emerged as novel PFOS substitutes. However, until now, limited data is available on their occurrence and environmental behaviors in the marine environment. Here, seawater and sediment samples were collected from East China Sea and analyzed for Cl-PFAESs, PFNOBS, and perfluoroalkyl acids (PFAAs; including their branched isomers) to investigate their concentrations, potential sources, and sediment-seawater partitioning behaviors.

The first mitochondrial genome of the genus Exhippolysmata (Decapoda: Caridea: Lysmatidae), with gene rearrangements and phylogenetic associations in Caridea.

The complete mitochondrial genome (mitogenome) of animals can provide useful information for evolutionary and phylogenetic analyses. The mitogenome of the genus Exhippolysmata (i.e.

Separation, characterization and hypoglycemic activity evaluation of a low molecular weight heteropolysaccharide from the fruiting body of .

Edible mushrooms have potential in anti-diabetic phytotherapy. They are rich in natural compounds such as polysaccharides, which have been known to have antihyperlipidemic effects since ancient times. A polysaccharide fraction of PP80 and a contained low molecular-weight (Mw), water-soluble polysaccharide (PPW-1, Mw: 3.

Occurrence of Free-Form and Conjugated Bisphenol Analogues in Marine Organisms.

Upon exposure, most bisphenol analogues (BPs) are rapidly metabolized to BP conjugates in organisms. Monitoring studies on BPs in aquatic organisms have been mainly focused on free-form BPs. However, the relative amount of conjugated BPs in organisms is still not well known, especially in marine organisms.

Occurrence and partitioning of polyhalogenated carbazoles in seawater and sediment from East China Sea.

Polyhalogenated carbazoles (PHCZs) have received great concern due to their environmental persistence and potential dioxin-like toxicities. Their presence in the marine sediment had been well characterized, but limited studies had investigated their environmental behaviors in the marine environment. In this study, we collected paired seawater (n = 48) and surface sediment samples (n = 48) from East China Sea and analyzed for carbazole (CZ) and 11 PHCZs to investigate the occurrence and spatial distribution of CZ and PHCZs in seawater and sediment, as well as to explore the partitioning behaviors of CZ and PHCZs between seawater and sediment.

Occurrence of phthalic acid esters in sediment samples from East China Sea.

Phthalic acid esters (PAEs) are widely used as plasticizers in many industrial and household products. The widespread distribution of PAEs in marine environment has attracted great concerns, due to their adverse health effects on marine organisms. However, the data on the occurrence of PAEs in sediment from East China Sea is still scarce.

Occurrence of phthalic acid esters in marine organisms from Hangzhou Bay, China: Implications for human exposure.

Owing to the wide application of phthalic acid esters (PAEs) in the manufacturing of plastic products, they are ubiquitous in the marine environment. However, the occurrence of various PAEs in marine organisms from China has not been well characterized. In this study, 341 marine organism samples (including fish, shrimp, crab, and shellfish) were collected from Hangzhou Bay, China and analyzed for 16 PAEs.

Physiological responses to cold and starvation stresses in the liver of yellow drum (Nibea albiflora) revealed by LC-MS metabolomics.

As global climate changes, mass mortality in farmed fish associated with the severely cold weather has aroused growing concerns. Yellow drum (Nibea albiflora) is an important maricultured fish in China, whereby its aquaculture suffered from overwinter mortality associated with cold and cold-induced-fasting stresses. Here, by using LC-MS metabolomics combined with transcriptomics, we investigated the physiological responses of yellow drum liver to cold and starvation stresses.

Identification of HSF1 Target Genes Involved in Thermal Stress in the Pacific Oyster Crassostrea gigas by ChIP-seq.

The Pacific oyster Crassostrea gigas, a commercially important species inhabiting the intertidal zone, facing enormous temperature fluctuations. Therefore, it is important to identify candidate genes and key regulatory relationships associated with thermal tolerance, which can aid the molecular breeding of oysters. Heat shock transcription factor 1 (HSF1) plays an important role in the thermal stress resistance.

Deciphering the Origin and Evolution of the XXY System in Two Closely-Related Species (Oplegnathidae and Centrarchiformes).

and . (Teleostei: Centrarchiformes: Oplegnathidae), are commercially important rocky reef fishes, endemic to East Asia. Both species present an XXY sex chromosome system.

Overwinter mortality in yellow drum (Nibea albiflora): Insights from growth and immune responses to cold and starvation stress.

The yellow drum (Nibea albiflora) is an economically important maricultured fish in China, but the aquaculture of this species has recently been limited by an increase in overwinter mortalities associated with cold and starvation stress due to global climate changes. To better understand the interaction between starvation and cold-stress-driven overwinter mortality, we investigated the effects of these stresses on the growth performance, liver lesions, and immune response of yellow drum fish. The fish were subjected to different cold treatments and under starvation stress.