Molecular surveillance of dengue virus in field-collected mosquitoes from Bhopal, central India: evidence of circulation of a new lineage of serotype 2.

Introduction: Dengue fever is hyperendemic in several Southeast and South Asian countries, including India, with all four serotypes (DENV 1-4) circulating at different periods and in different locations. Sustainable and improved virological and entomological surveillance is the only tool to prevent dengue and other vector-borne diseases.

Objectives: The present study has been carried out to detect and characterize the circulating dengue virus (DENV) in field-collected mosquitoes in Bhopal, Central India.

Dynamic Multicomponent Reactions-Directed Self-Assembled G-quadruplex Inherent Antibacterial Hydrogel.

Nowadays, inherent antibacterial hydrogels have gained significant attention due to their utilization against infectious bacteria. Herein, we focus on the development of an injectable, self-healable, dynamic, and G-quadruplex hydrogel with inherent antibacterial activity. The dynamic self-assembled hydrogel is constructed upon multicomponent reactions (MCR) among guanosine, 2-formylphenylboronic acid, and amino acid/peptides in the presence of potassium ions.

Nucleopeptide-Coupled Injectable Bioconjugated Guanosine-Quadruplex Hydrogel with Inherent Antibacterial Activity.

The multicomponent reaction-directed self-assembled hydrogels offer the opportunities to fabricate materials with ubiquitous properties which sometimes are not possible to generate from single components. Therefore, multicomponent-derived hydrogels have enormous applications in biomedical fields, and the number of such systems is increasing day by day. Herein, the multicomponent self-assembly techniques have been employed to develop a biomimetic low-molecular-weight G-quadruplex hydrogel under physiological conditions.

Next-generation nutraceuticals: bioactive peptides from plant proteases.

Bioactive peptides are short and specific fragments of proteins with a wide range of biological activities that provide health benefits to the host. These natural peptides are safe and nontoxic and do not show any side effects. Nowadays, the production and characterization of bioactive peptides have been a key area of research as they show great potential as nutraceuticals and functional foods.

Redefining genomic view of through pangenome analysis and identification of drug targets from its core genome.

Introduction:: infection (CDI) is a leading cause of gastrointestinal infections and in the present day is a major concern for global health care system. The unavailability of specific antibiotics for CDI treatment and its emerging cases worldwide further broaden the challenge to control CDI.

Methods:: The availability of a large number of genome sequences for and many bioinformatics tools for genome analysis provides the opportunity for pangenomic analysis.

Tapping Into Actinobacterial Genomes for Natural Product Discovery.

The presence of secondary metabolite biosynthetic gene clusters (BGCs) makes actinobacteria well-known producers of diverse metabolites. These ubiquitous microbes are extensively exploited for their ability to synthesize diverse secondary metabolites. The extent of their ability to synthesize various molecules is yet to be evaluated.

Microbial alkaline serine proteases: Production, properties and applications.

Proteolytic enzymes hold a pivotal position in numerous industrial processes where hydrolysis of protein molecules is required under precise conditions. The emerging trend of biotechnological applications in recent years has witnessed a renewed interest in alkaline serine proteases extending their utility in detergent, leather, textile, food and pharmaceutical industries. A variety of microorganisms have been reported to produce alkaline serine proteases on a large scale, however, extensive research to find an alkaline serine protease with desirable characteristics such as significant catalytic efficiency, expanded stability and broad substrate specificity is still ongoing.

A novel thiol-dependent serine protease from sp. N1.

Alkaline proteases have several industrial applications. In the present study, newly isolated sp. N1 was screened as hyper producer of serine protease A multimeric protease of the fungus was purified to homogeneity till 96.

Gamma irradiation induced effects on shoot cultures and influence of monochromatic light regimes on irradiated shoot cultures of orchid.

The effects of gamma radiation and monochromatic lights on growth of shoot cultures of orchid, were investigated. The gamma irradiated shoot cultures grown under white, blue, yellow and red monochromatic lights exhibited differential growth pattern. Shoot cultures gamma irradiated at 15-45 Gy showed reduced shoot length, fresh weight and leaf area.

Pectinolytic enzymes-solid state fermentation, assay methods and applications in fruit juice industries: a review.

A plethora of solid substrates, cultivation conditions and enzyme assay methods have been used for efficient production and estimation of polygalacturonase and pectin methylesterase enzymes. Recent developments in industrial biotechnology offer several opportunities for the utilization of low cost agro-industrial waste in Solid State Fermentation (SSF) for the pectinolytic enzyme production using fungi. Fruit waste mainly citrus fruit waste alone and along with other agro-industrial waste has been explored in SSF for enzyme production.

Optimization of Fermentation Medium for the Production of Glucose Isomerase Using Streptomyces sp. SB-P1.

The combination of medium ingredients has a profound influence on the metabolic pathways running in the microorganism which regulates the production of numerous metabolites. Glucose isomerase (GI), an enzyme with huge potential in the market, can isomerise glucose into fructose. GI is used widely for the production of High-Fructose Corn Syrup (HFCS).