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An improved counterselection cassette for use in Haemophilus influenzae.
Gene
January 2012
Department of Microbiology, Immunology, & Molecular Genetics, University of Kentucky, MS-423 Chandler Medical Center, Lexington, KY 40536-0298, USA.
Article Synopsis
- Counterselectable cassettes are valuable tools in molecular biology for making deletion mutants or point mutations without visible markers.
- The author developed an inducible sacB cassette that operates with a tetracycline repressor to enhance precision in genetic manipulation.
- When paired with a kanamycin-resistance marker, this system effectively created unmarked mutants in Haemophilus influenzae while minimizing typical issues related to sacB use in counterselection.