Targeted Plasma Bile Acid Metabolomic Analysis in Metabolic Dysfunction-Associated Steatohepatitis and Alcoholic Hepatitis.

Even though many metabolic liver diseases can now be diagnosed using blood tests and diagnostic imaging, early diagnosis remains difficult. Understanding mechanisms contributing to the progression from Metabolic Dysfunction-Associated Steatohepatitis (MASH) and Alcoholic Hepatitis (AH) to cirrhosis is critical to reduce the burden of end-stage liver disease. Monitoring individual bile acids has been proposed as a way to distinguish various liver disorders.

Prognostic Value of CXCL13, CCL11, and CCL20 Chemokines in Multiple Sclerosis.

The course of relapsing-remitting multiple sclerosis (RRMS) is highly variable and there is a lack of effective prognostic biomarkers. This study aimed to assess the potential prognostic value of the chemokines B lymphocyte chemoattractant molecule (CXCL13), eotaxin-1 (CCL11), and macrophage inflammatory protein 3-alpha (CCL20) in RRMS. Forty-two patients with MS were enrolled, along with 22 controls, 12 of the controls were idiopathic intracranial hypertension (IIH) patients, and 10 of the controls were other neurologic diseases (OND).

Comprehensive Metabolomic Profiling in Adults with X-Linked Hypophosphatemia: A Case-Control Study.

Background: X-linked hypophosphatemia (XLH) is a rare disorder characterized by elevated levels of fibroblast growth factor 23 (FGF-23), leading to hypophosphatemia and complications in diagnosis due to its clinical heterogeneity. Metabolomic analysis, which examines metabolites as the final products of cellular processes, is a powerful tool for identifying in vivo biochemical changes, serving as biomarkers of pathological abnormalities, and revealing previously uncharted metabolic pathways.

Methods: A multicenter cross-sectional case-control study of adult patients diagnosed with XLH was conducted.

The First Report of Environmental Exposure to Barium in 10 Localities Close to Industrial Areas and Ports in the Amazon.

Environmental exposure to metallic contaminants such as barium (Ba) is a worldwide concern, as these metals can even be toxic to the human body. Data on different sources of exposure to Ba and possible routes of entry are important for preventing adverse health effects. Blood Ba levels were evaluated in 10 localities in the cities of Barcarena and Abaetetuba in the Amazon.

Exploring the Active Constituents of in Protecting the Skin Barrier and the Synergistic Effects with Collagen XVII.

is mainly used to treat skin inflammations, wounds, and infections. In this study, Andrographis Herba, the aerial part of the plant, was proven to increase the viability of UVB-damaged HaCat cells and reduce reactive oxygen species levels. The chemical composition of Andrographis Herba extract (AHE) was analyzed using UPLC-Q-TOF-MS, and diterpene lactones were identified as its primary constituents.

The Role of Extracts of Edible Parts and Production Wastes of Globe Artichoke ( L. var. (L.)) in Counteracting Oxidative Stress.

In addition to the immature edible flower heads, the cultivation of globe artichoke ( L. var. (L.

Structural Characterization of Foxtail Millet () Polysaccharides and Evaluation of Its Antioxidant and Immunostimulatory Activities.

A water-soluble polysaccharide from foxtail millet (FM-D1) was isolated and purified through gradient ethanol precipitation and column chromatography. Size-Exclusion Chromatography-Multi-Angle Light Scattering-Refractive Index (SEC-MALLS-RI) and high-performance anion-exchange chromatography (HPAEC) analyses revealed that FM-D1 constitutes a highly purified neutral polysaccharide exclusively composed of glucose as the sugar unit, with a molecular weight of 14.823 kDa.

Natural Deep Eutectic Solvents as Green Alternatives for Extracting Bioactive Compounds from Taxa with Potential Cosmetic Applications.

This study investigated the potential of natural deep eutectic solvents (NADESs) for extracting bioactive compounds from the aerial parts of two mountain tea taxa, ssp. (Boiss. & Heldr.

Chemical Composition of Mexicali Propolis and Its Effect on Gastric Repair in an Indomethacin-Induced Gastric Injury Murine Model.

Propolis is a resinous substance produced by bees that has several biomedical properties that could contribute to the repair process of the gastric mucosa, such as antioxidant, anti-inflammatory, healing, and gastroprotective properties. Thus, this study aimed to determine the chemical composition of Mexicali propolis, its antioxidant capacity, and its effect on gastric repair. Three polarity-directed extracts were obtained: the ethanolic extract, the ethyl acetate extract, and the hexane extract.

GENI as an AMPK Activator Binds α and γ Subunits and Improves the Memory Dysfunction of Alzheimer's Disease Mouse Models via Autophagy and Neuroprotection.

Geniposidic 4-isoamyl ester (GENI) with anti-aging effects is a new iridoid glycoside derivative from Ellis found in our previous study. In this study, to indicate whether this compound has anti-Alzheimer's disease (AD) effect, the galactose-induced AD mice and naturally aging mice with AD were used to do drug efficacy evaluation. Furthermore, the Western blot, small interfering RNA (siRNA), drug affinity responsive target stability (DARTS), cellular thermal shift assay (CESTA), liquid chromatography-tandem mass spectrometry (LC/MS-MS), adenosine 5'-monophosphate-activated protein kinase (AMPK) mutants and surface plasmon resonance (SPR) analysis were utilized to clarify the mechanism of action and identify target protein of this molecule.

Chick Early Amniotic Fluid Alleviates Dextran-Sulfate-Sodium-Induced Colitis in Mice via T-Cell Receptor Pathway.

Ulcerative colitis (UC) is a chronic immune disease that is difficult to cure. We recently found that chick early amniotic fluid (ceAF) has notable anti-inflammatory and antioxidative properties, through its active components. This study demonstrates the potential of ceAF as a protective agent against UC.

Effects of the Maximum Recommended Levels of Fumonisins in the EU on Oxylipin Profiles in the Liver and Brain of Chickens.

This study aimed to assess the effects of a diet containing 20.8 mg FB1 + FB2/kg over four and nine days on oxylipin (OL) profiles in the liver and brain of chickens. A total of 96 OLs, derived from seven polyunsaturated fatty acids (PUFAs) via the cyclooxygenase (COX), lipoxygenase (LOX), cytochrome P450 (P450), and non-enzymatic pathways, were measured using HPLC-MS/MS.

Essential Oils from Citrus Peels Promote Calcium Overload-Induced Calcicoptosis in U251 Cells.

Citrus peel essential oils (CPEOs) have demonstrated substantial medicinal potential for glioblastoma treatment because of their extensive antitumor effects, low potential for drug resistance, and ability to cross the human blood-brain barrier. In this study, the chemical compositions of five CPEOs were analyzed via gas chromatography-mass spectrometry (GC-MS). CCK8 assays were used to evaluate the ability of five CPEOs to inhibit U251 human glioblastoma cells, and XLB and RA were selected for further investigation.

Parameterization of the Differences in Neural Oscillations Recorded by Wearable Magnetoencephalography for Chinese Semantic Cognition.

Neural oscillations observed during semantic processing embody the function of brain language processing. Precise parameterization of the differences in these oscillations across various semantics from a time-frequency perspective is pivotal for elucidating the mechanisms of brain language processing. The superlet transform and cluster depth test were used to compute the time-frequency representation of oscillatory difference (ODTFR) between neural activities recorded by optically pumped magnetometer-based magnetoencephalography (OPM-MEG) during processing congruent and incongruent Chinese semantics.

Morphological, Physiological, and Transcriptional Changes in L. Under In Vitro Polyethylene Glycol-Induced Water Stress.

Saffron ( L.), a perennial geophyte from the Iridaceae family, blooms in autumn and thrives in Mediterranean-like climates. It is highly valued for its therapeutic and commercial uses.

Antibacterial Potential of Honeybee Venom and Extracellular Metabolites Against Multidrug-Resistant Pathogenic Bacteria.

Antimicrobial resistance (AMR) poses a critical global health threat, driving the search for alternative treatments to conventional antibiotics. In this study, the antibacterial properties of honeybee venom (BV) and fungal red dye (RD) were evaluated against three multidrug-resistant bacterial pathogens. Extracts of BV and RD exhibited dose-dependent antibacterial activity against the three tested bacteria, with their strongest effectiveness against (minimum inhibitory concentrations [MIC] = 3.

Effects of Acute Lateral Ankle Sprain on Spinal Reflex Excitability and Time-to-Boundary Postural Control in Single-Leg Stance.

: Acute lateral ankle sprain (ALAS) affects balance, often assessed by changes in traditional center of pressure (COP) parameters. Spatiotemporal measures of COP and time-to-boundary (TTB) analysis may offer improved sensitivity in detecting postural deviations associated with ALAS. However, the neurophysiological mechanism underlying these changes remains unknown.

Seasonal Changes in the Elemental Composition of Five Valuable Fish Species () from Bozcaada, North Aegean Sea: A Health Risk and Nutritional Benefit Assessment.

This study investigates the seasonal variations in the elemental composition of five economically valuable fish species from Bozcaada, North Aegean: red seabream (), gilthead seabream (), saddled seabream (), white seabream (), and common dentex (), with a focus on both essential minerals and toxic metals. Fish samples ( = 10 per species per season) were collected across four seasons, and their weights and lengths were recorded. The concentrations of elements such as calcium, potassium, magnesium, phosphorus, copper, iron, manganese, zinc, chromium, nickel, selenium, cadmium, and mercury were analyzed using Inductively Coupled Plasma Mass Spectrometry (ICP-MS).

Efficacy of Household and Commercial Washing Agents in Removing the Pesticide Thiabendazole Residues from Fruits.

Pesticide residues on fruits pose a global food safety concern, emphasizing the need for effective and practical removal strategies to ensure safe consumption. This study investigates the efficacy of household ingredients (corn starch, all-purpose flour, rice flour and baking soda) and four commercial fresh produce wash products in eliminating a model pesticide thiabendazole with and without a model non-ionic surfactant Alligare 90 from postharvest fruits. Surface-enhanced Raman spectroscopy (SERS) was employed for the rapid, in situ quantification of residue removal on apple surfaces.

Pomegranate Juices: Analytical and Bio-Toxicological Comparison of Pasteurization and High-Pressure Processing in the Development of Healthy Products.

Two different produced and packaged commercial typologies of pomegranate juice were analyzed for their physicochemical, nutritional, and biological properties. The effects of classical pasteurization (PJ) and high-pressure processing (HP), applied during the productive cycle, were evaluated through several advanced analytical methods, such as CIEL*a*b* colorimetry, HPLC-DAD, DI-ESI-MS and MS/MS, and NMR analyses. Moreover, the exerted biological activity of the two pomegranate juices was monitored through Total Phenolic and Total Flavonoid Contents, antiradical, antioxidant and chelating activity.

Comparing the Structural and Physicochemical Properties of Highland Barley -Glucan from Different Sources: A Focus on Color.

Herein, -glucan (BG) was extracted from different colored varieties of highland barley (HB, ), defined as BBG, WBG, and LBG depending on the colors of black, white, and blue and their molecular structure and physicochemical properties were investigated through a series of technical methods. The high-performance anion-exchange chromatography (HPAEC) results indicated the extracted BBG, LBG, and WBG mainly comprised glucose regardless of color. The molecular weight (M) of BBG, LBG, and WBG were 55.

Simultaneous Determination of Three Active Forms of Vitamin B12 In Situ Produced During Fermentation by LC-MS/MS.

The in situ fortification of vitamin B12 (VB12) in foods through fermentation is an effective strategy to address the deficiency of this micronutrient, and precise monitoring of VB12 production is crucial for developing VB12-fortified functional foods. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is advantageous for analyzing trace substances in food due to its high sensitivity. In the present study, an LC-MS/MS method capable of rapidly and accurately quantifying three active forms of VB12, namely adenosylcobalamin (AdoCbl), methylcobalamin (MeCbl), hydroxocobalamin (OHCbl), in 8 min were developed.

Changes in the Flavor of Cold-Pressed Tiger Nut Oil ( L.) During Refining Processes and Evaluation of Its Thermal Properties.

Oil extracted from tiger nut is a good, edible source owing to its richness in unsaturated fatty acids. This study investigated the effects of the refining processes on the flavor components of crude tiger nut oil by GC-MS and focused on the thermal stability of the refined oil under high-temperature conditions. Three different refining processes were evaluated: citric acid-assisted hydration degumming, alkali deacidification and bleaching.

Recovery of Bioactive Constituents from Olive Leaf Pruning Waste of Five Different Cultivars: A Comparison of Green Extraction Techniques to Maximize Health Benefits.

Sustainable agro-waste revaluation is critical to enhance the profitability and environmental footprint of the olive oil industry. Herein, the valorization of olive leaf pruning waste from five cultivars ('Caiazzana', 'Carolea', 'Itrana', 'Leccino', and 'Frantoio') employed green extraction methods to recover compounds with potential health benefits. Sequential ultrasound-assisted maceration (UAM) in -hexane and ethanol was compared with a compressed fluid extraction strategy consisting of supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE) for their efficiency in recovering distinct classes of bioactives.

Oxidative Stability of Virgin Avocado Oil Enriched with Avocado Leaves and Olive-Fruit-Processing By-Products (Leaves, Pomace) via Ultrasound-Assisted Maceration.

Virgin avocado oil (VAO), treasured for its nutritional and sensory properties, is susceptible to oxidation. To improve its oxidative stability, the feasibility of enrichment with antioxidants from avocado or olive-processing by-products via ultrasound-assisted maceration was explored. Dried, milled avocado (AL), olive leaves (OL), or olive pomace (OP) were ultrasound-macerated with laboratory-extracted VAO at 5, 10, and 20% levels.