Effects of Hot Air, Vacuum, and Conductive Drying on the Fatty Acid Profile of Pulp and Its Processing By-Products.

Considering the short shelf life of fresh pumpkin due to its high water content and the extensive use of dried pumpkin in the food industry, it is necessary to find an efficient drying method that minimizes water activity and preserves nutritional properties. In this study, the effects of hot air drying (HAD), vacuum drying (VAD), and conductive drying (CD) at 50, 60, and 70 °C on fatty acid profiles were investigated to determine optimal drying conditions that preserve fatty acid (FA) quality and associated nutritional benefits. Results showed that drying methods had a significant effect ( < 0.

Correlating Microbial Dynamics with Key Metabolomic Profiles in Three Submerged Culture-Produced Vinegars.

Although vinegar is a product obtained by a well-known bioprocess from a technical point of view, the complex microbiota responsible for its production and their involvement in the organoleptic profiles are not clear yet. In this work, three acetification profiles in submerged culture using both synthetic and raw materials from Andalusia (Spain) were characterized by metagenomic (16S rRNA amplicon sequencing) and metabolomic tools (stir-bar sorptive extraction with thermo-desorption coupled to gas chromatography-mass spectrometry (SBSE-TD-GC-MS) and high-performance liquid chromatography (HPLC)). A total of 29 phyla, 208 families, and many more genera were identified, comprising bacteria and archaea as well as 75 metabolites, including minor volatile compounds, amino acids, biogenic amines, and other nitrogenous compounds.

Chemical Profile and Antibacterial Activity of L. cv Graciano Pomace Extracts Obtained by Green Supercritical CO Extraction Method Against Multidrug-Resistant Strains.

The objectives of this study were to obtain and characterise polyphenolic extracts from red grape pomace of L. cv Graciano via conventional solvent extraction (SE) and green supercritical fluid extraction (SFE) and to evaluate their antibacterial activity against susceptible and multidrug-resistant strains of intestinal origin. The SE and SFE methods were optimised, and ultra-performance liquid chromatography/mass spectrometry (UPLC/QqQ-MS/MS) analysis revealed 38 phenolic compounds in the SE sample, with anthocyanins being the predominant polyphenols, and 21 phenolic compounds in the SFE samples, among which hydroxybenzoic acids and flavonols were the predominant compounds.

Differences in the Quality Components of Wuyi Rock Tea and Huizhou Rock Tea.

Different origins and qualities can lead to differences in the taste and aroma of tea; however, the impacts of origin and quality on the taste and aroma characteristics of Wuyi rock tea and Huizhou rock tea have rarely been studied. In this study, high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and sensory evaluation methods were used to compare the quality components of Wuyi rock tea and Huizhou rock tea. The sensory evaluation showed that they each have their own characteristics, but the overall acceptability of Wuyi rock tea is ahead of Huizhou rock tea ( < 0.

Study on the Chemical Composition and Multidrug Resistance Reversal Activity of (Euphorbiaceae).

belongs to the family Euphorbiaceae and is widely distributed in northern Xinjiang, making it a characteristic plant of the region in Xinjiang, China. The chemical composition and biological activity of have not yet been reported, although certain compounds isolated from plants in Xinjiang, China, have demonstrated exceptional multidrug resistance (MDR) reversal. This study aims to investigate the chemical components present in with the potential to reverse MDR.

Transcriptomic and Proteomic Analysis of Monkeypox Virus A5L-Expressing HEK293T Cells.

Monkeypox (MPOX) is a zoonotic viral disease caused by the Monkeypox virus (MPXV), which has become the most significant public health threat within the genus since the eradication of the Variola virus (VARV). Despite the extensive attention MPXV has garnered, little is known about its clinical manifestations in humans. In this study, a high-throughput RNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach was employed to investigate the transcriptional and metabolic responses of HEK293T cells to the MPXV A5L protein.

Bioactive Phenolics of L. Subsp. Falezlez: A Molecular and Biochemical Approach to Antioxidant and Urease Inhibitory Activities.

This study examines the chemical composition, antioxidant properties, and urease inhibitory effects of L. subsp. falezlez (Coss.

Association of Baseline Lipopolysaccharide-Binding Protein with Expanded Disability Status Score Dynamics in Patients with Relapsing-Remitting Multiple Sclerosis: A Pilot Study.

Forecasting the progression of the disease in the early inflammatory stage of the most prevalent type of multiple sclerosis (MS), referred to as relapsing-remitting multiple sclerosis (RRMS), is essential for making prompt treatment modifications, aimed to reduce clinical relapses and disability. In total, 58 patients with RRMS, having an Expanded Disability Status Scale (EDSS) score less than 4, were included in this study. Baseline magnetic resonance imaging (MRI) was performed, and brain and spinal cord lesions were evaluated.

Transgenic Cotton Expressing ds Significantly Delays the Growth and Development of by Inhibiting Its Glycolysis and TCA Cycle.

In our previous research, we found that not only participates in the detoxification metabolism of neonicotinoid insecticides in cotton aphid but also affects their growth and development. However, how does transgenic cotton expressing ds affect the growth and development of cotton aphid? In this study, we combined transcriptome and metabolome to analyze how to inhibit the growth and development of cotton aphid treated with transgenic cotton expressing ds (TG cotton). The results suggested that a total of 509 differentially expressed genes (DEGs) were identified based on the DESeq method, and a total of 431 differential metabolites (DAMs) were discovered using UPLC-MS in the metabolic analysis.

Structural Characterization and Immune Activation Capacity of Peptidoglycan from in RAW264.7 Cells.

Peptidoglycan (PGN) is a unique component of prokaryotic cell walls with immune-enhancing capacities. Here, we extracted PGN from , a by-product of amino acid fermentation, using the trichloroacetic acid (TCA) method. SDS-PAGE analysis confirmed the presence of PGN, with a band of approximately 28 kDa.

Lipid Profile of Mayr in Adaptation to Natural Conditions in the Cryolithozone.

The prevalence of coniferous trees in the forest landscapes of northeastern Siberia is conditioned by their high frost resistance. The Kajander larch ( Mayr), which can survive under natural conditions (down to -60 °C) in the cryolithozone of Yakutia, is the dominant forest-forming species. We hypothesise that our study using HPTLC-UV/Vis/FLD, TLC-GC/FID, and GC-MS methods of seasonal features of the lipid profile of Kajander larch tissues will bring us closer to understanding the mechanisms of participation of lipid components in the adaptation of this valuable tree species to the cold climate of the cryolithozone.

Cytotoxic ROS-Consuming Mn(III) Synzymes: Structural Influence on Their Mechanism of Action.

ROS (i.e., reactive oxygen species) scavenging is a key function of various Mn-based enzymes, including superoxide dismutases (SODs) and catalases, which are actively linked to oxidative stress-related diseases.

Newly Initiated Statin Treatment Is Associated with Decreased Plasma Coenzyme Q10 Level After Acute ST-Elevation Myocardial Infarction.

Coenzyme Q10 (CoQ10) plays a crucial role in facilitating electron transport during oxidative phosphorylation, thus contributing to cellular energy production. Statin treatment causes a decrease in CoQ10 levels in muscle tissue as well as in serum, which may contribute to the musculoskeletal side effects. Therefore, we aimed to assess the effect of newly initiated statin treatment on serum CoQ10 levels after acute ST-elevation myocardial infarction (STEMI) and the correlation of CoQ10 levels with key biomarkers of subclinical or clinically overt myopathy.

Pulsed Electric Field (PEF) Treatment Results in Growth Promotion, Main Flavonoids Extraction, and Phytochemical Profile Modulation of Georgi Roots.

This study aims to explore the effect of pulsed electric field (PEF) treatment as a method very likely to result in reversible electroporation of Georgi underground organs, resulting in increased mass transfer and secondary metabolites leakage. PEF treatment with previously established empirically tailored parameters [E = 0.3 kV/cm (U = 3 kV, d = 10 cm), t = 50 µs, N = 33 f = 1 Hz] was applied 1-3 times to roots submerged in four different Natural Deep Eutectic Solvents (NADES) media (1-choline chloride/xylose (1:2) + 30% water, 2-choline chloride/glucose (1:2) + 30% water, 3-choline chloride/ethylene glycol (1:2), and 4-tap water (EC = 0.

Optimization of a Pressurized Extraction Process Based on a Ternary Solvent System for the Recovery of Neuroprotective Compounds from Leaves.

Green chemistry focuses on reducing the environmental impacts of chemicals through sustainable practices. Traditional methods for extracting bioactive compounds from leaves, such as hydro-distillation and organic solvent extraction, have limitations, including long extraction times, high energy consumption, and potential toxic solvent residues. This study explored the use of supercritical fluid extraction (SFE), pressurized liquid extraction (PLE), and gas-expanded liquid (GXL) processes to improve efficiency and selectivity.

Quantitative Determination of a Series of Oxysterols by an Optimized LC-MS/MS Analysis in Different Tissue Types.

Oxysterols, as metabolites of cholesterol, play a key role in cholesterol homeostasis, autophagosome formation, and regulation of immune responses. Disorders in oxysterol metabolism are closely related to the pathogenesis of neurodegenerative diseases. To systematically investigate the profound molecular regulatory mechanisms of neurodegenerative diseases, it is necessary to quantify oxysterols and their metabolites in central and peripheral biospecimens simultaneously and accurately.

Influence of Kunth Flavonoids on Composition of Soil Microbial Community.

, one of the world's most destructive invasive species, is known for causing significant ecological and economic harm. While extensive research has focused on its growth characteristics, secondary metabolites, and control measures, its chemical interactions with the environment-particularly the role of flavonoids in shaping soil microbial communities-remain underexplored. In this study, we identified and quantified ten flavonoids from root exudates using UPLC-MS, including Hispidulin, Isorhamnetin, and Mikanin.

A Lupin () Protein Hydrolysate Decreases the Severity of Experimental Autoimmune Encephalomyelitis: A Preliminary Study.

Multiple sclerosis (MS) is a neurodegenerative disease, with inflammation and oxidative stress in the central nervous system being the main triggers. There are many drugs that reduce the clinical signs of MS, but none of them cure the disease. Food proteins have been shown to contain encrypted peptides that can be released after hydrolysis and exert numerous biological activities.

Urinary Proteome Characterization of Stroke-Prone Spontaneously Hypertensive Rats.

Hypertension is a multifactorial and complex disease influenced by genetic and environmental factors, and it has become one of the most serious public health challenges. This study aimed to investigate the changes in hypertension based on urinary proteome. The stroke-prone spontaneously hypertensive rats (SHRSPs) model was used to examined urinary proteome changes during the development of hypertension.

Shape-Persistent Tetraphenylethylene Macrocycle: Highly Efficient Synthesis and Circularly Polarized Luminescence.

Circularly polarized luminescence (CPL) is an emerging field with significant applications in molecular electronics, optical materials, and chiroptical sensing. Achieving efficient CPL emission in organic systems remains a major challenge, particularly in the development of materials with high fluorescence quantum yields (Φ) and large luminescence dissymmetry factors (g). Herein, we report the efficient synthesis of shape-persistent tetraphenylethylene macrocycles and investigate its potential as a CPL material.

Enhancement of Air-Entrained Grout-Enriched Vibrated Cemented Sand, Gravel and Rock (GECSGR) for Improving Frost and Thawing Resistance in CSGR Dams.

Cemented Sand, Gravel, and Rock (CSGR) dams have traditionally used either Conventional Vibrated Concrete (CVC) or Grout-Enriched Roller Compacted Concrete (GERCC) for protective and seepage control layers in low- to medium-height dams. However, these methods are complex, prone to interference, and uneconomical due to significant differences in the expansion coefficient, elastic modulus, and hydration heat parameters among CSGR, CVC, and GERCC. This complexity complicates quality control during construction, leading to the development of Grout-Enriched Vibrated Cemented Sand, Gravel, and Rock (GECSGR) as an alternative.

Features of Structure and Flow Field in Homemade Co-Current Cavitation Water Jet Nozzle.

The cavitation water jet cleaning and coating removal technique represents an innovative sustainable method for cleaning and removing coatings, with the nozzle serving as a crucial component of this technology. Developing an artificially submerged nozzle with a reliable structure and excellent cavitation performance is essential for enhancing cavitation water jets' cleaning and coating removal efficacy in an atmosphere environment (non-submerged state). This study is based on the shear flow cavitation mechanism of an angular nozzle, the resonance principle of an organ pipe, and the jet pump principle.

Erosion-Corrosion Behavior of 316L Stainless Steel in Chloropropene.

The objective of this study is to investigate the impact of different pH values and chloropropene flow rates on the erosion-corrosion behavior of 316L stainless steel. The influence of various factors on the surface morphology was analyzed using scanning electron microscopy, X-ray powder diffractometry, and electrochemical impedance spectroscopy techniques. The results revealed that at a pH value of 3.

Synthesis, Characterization, and Investigation of Corona Formation of Dipeptide-Based Nanomaterials.

Peptide-based nanomaterials can be easily functionalized due to their functional groups, as well as being biocompatible, stable under physiological conditions, and nontoxic. Here, diphenylalanineamide-based nanomaterials (FFANMs) were synthesized, decorated with Ca ions to set the surface charge, and characterized for possible use in gene delivery and drug release studies. FFANMs were characterized by SEM, TEM, dynamic light scattering (DLS), and LC-MS/MS.

A Time Series Proposal Model to Define the Speed of Carbon Steel Corrosion in an Extreme Acid Environment.

This article shows the behavior of the corrosive effect of acid mine water on carbon steel metal alloys. Mining equipment, composed of various steel alloys, is particularly prone to damage from highly acidic water. This corrosion results in material thinning, brittle fractures, fatigue cracks, and ultimately, equipment failure.