MicroRNAs Expression Profile in MN1-Altered Astroblastoma.

Background/objectives: Astroblastoma is a rare glial neoplasm more frequent in young female patients, with unclear clinical behaviors and outcomes. The diagnostic molecular alteration is a rearrangement of the Meningioma 1 () gene. MicroRNAs (miRNAs) are important gene expression regulators with strong implications in biological processes.

Advancements in Cardiac Amyloidosis Treatment.

Cardiac amyloidosis (CA) is a progressive condition resulting from the deposition of amyloid fibrils in the heart, which leads to severe diastolic dysfunction and restrictive cardiomyopathy. The disease has two main subtypes: light-chain and transthyretin (TTR) CA, with the latter subdivided into wild-type and hereditary forms. Despite advances in diagnostic imaging, early detection remains a challenge due to non-specific symptoms that mimic other cardiac conditions.

Emerging Trends in Non-Invasive Brain Stimulation: The New Kids on the Block.

In the Sustainable Development Goals of the United Nations for 2030, mental health has been identified as a global priority, emphasizing the need to reduce the prevalence, morbidity, and premature mortality associated with mental disorders [...

"My Husband Is a 'Mama's Boy'": Women's Views on Male Engagement in Maternal, Newborn, and Child Health in Western Kenya.

There is an increasing global acknowledgment of the critical role that men have as key partners in maternal, newborn, and child health (MNCH). Most male-engagement initiatives do not address the perceived benefits and risks that women may experience because of increased male participation in MNCH, especially in Kenya. The aim of this study, therefore, is to qualitatively assess how women perceive and experience increased male engagement in MNCH in western Kenya.

"Lack" and "Finally": A Qualitative Analysis of Barriers and Facilitators in Rare Disease Healthcare.

This study explored the experiences of adults with diverse rare diseases (RDs) and RD caregivers with barriers and facilitators to healthcare access in the United States (US), including during the early part of the COVID-19 pandemic, and their recommendations for improving access. Adults with RDs and parents/caregivers to children with RDs (N = 1128) completed open-ended survey items. Responses were analyzed using thematic analysis.

Climate and Soil Properties Drive the Distribution of Minor and Trace Elements in Forest Soils of the Winter Olympic Core Area.

Minor and trace elements in soil play a crucial role in regulating ecological processes that sustain the functionality of forest ecosystems. In this study, we have selected three conifer forests (, , ), one broadleaf forest () and one mixed forest of and in the Winter Olympic core area and determined the pattern of 12 typical elements (B, Fe, V, Cr, Ni, Co, Mn, As, Cu, Zn, Sn and Se) in soils and their main drivers in the three different soil layers (A, B and C horizon) in each soil profile. Our results showed that the concentrations of B, Fe, Cr, Cu, Ni and Sn were mainly enriched in the broadleaf forest and mixed broadleaf-conifer forest zones, and the average concentrations of Co, Mn, V, Zn, As and Se were mainly enriched in coniferous forest zones in contrast.

Antarctic Toothfish in the South Orkney Islands: Using Otolith Chemistry to Test Current Hypotheses About Nursery Areas and Demographic Units.

We used otolith chemistry to test and complement current hypotheses regarding habitat use and connectivity between sub-populations in Area 48 of the Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR). Sagittal otoliths from 45 fish sampled near the South Orkney Islands were analysed. Their elemental (Li, Na, Mg, Cr, Mn, Sr, Sn, and Ba relative to Ca) and isotopic (δO and δC) signatures were examined in both the nuclear and marginal regions, representing juvenile and adult stages.

Diagnostic Accuracy of Detective Flow Imaging Endoscopic Ultrasonography for Evaluating Blood Flow Within Mural Nodules of Intraductal Papillary Mucinous Neoplasms.

Detective flow imaging (DFI) endoscopic ultrasonography (EUS) can identify the microvascular flow imaging of a mural nodule (MN) in an intraductal papillary mucinous neoplasm (IPMN) without the use of contrast agents. This retrospective study evaluated the diagnostic accuracy of DFI-EUS and its ability to evaluate the blood flow of MNs in IPMNs. Between April 2021 and September 2023, 68 patients with MNs in IPMNs observed on EUS images were retrospectively analyzed.

Seasonal Changes in the Elemental Composition of Five Valuable Fish Species () from Bozcaada, North Aegean Sea: A Health Risk and Nutritional Benefit Assessment.

This study investigates the seasonal variations in the elemental composition of five economically valuable fish species from Bozcaada, North Aegean: red seabream (), gilthead seabream (), saddled seabream (), white seabream (), and common dentex (), with a focus on both essential minerals and toxic metals. Fish samples ( = 10 per species per season) were collected across four seasons, and their weights and lengths were recorded. The concentrations of elements such as calcium, potassium, magnesium, phosphorus, copper, iron, manganese, zinc, chromium, nickel, selenium, cadmium, and mercury were analyzed using Inductively Coupled Plasma Mass Spectrometry (ICP-MS).

Composite Flours Based on Black Lentil Seeds and Sprouts with Nutritional, Phytochemical and Rheological Impact on Bakery/Pastry Products.

This paper aimed to study the nutritional, phytochemical and rheological properties of some composite flours based on wheat flour (WF) mixed with non-germinated (LF) and sprouted lentil flour (SLF), in order to fortify the wheat flour and to obtain functional bakery/pastry products. The composite flours based on wheat flour and bean lentil flour (BLWF) and sprouted lentil flour (SLWF) were analyzed from the point of view of proximate composition (proteins, lipids, total carbohydrates, and minerals), content of individual and total polyphenols (TPC), as well as the contents of macro and microelements. For use in baking/pastries, the composite flours were tested from the point of view of rheological behavior using the MIXOLAB system, and the profiles obtained were compared with those of bread and biscuit.

Physicians' knowledge, attitudes, and beliefs regarding practices of male and female surgical sterilization procedures in Lebanon.

Objective: Surgical sterilization, including vasectomy in males and tubal ligation in females, is a highly effective but underutilized contraception method. Adoption rates vary globally mostly due to misconceptions by both the general public and practicing physicians. Our survey aims to explore physicians' knowledge, attitudes and beliefs about surgical sterilization techniques in Lebanon.

Structural and topological analysis of thiosemicarbazone-based metal complexes: computational and experimental study of bacterial biofilm inhibition and antioxidant activity.

The structural and electronic behavior of thiosemicarbazone (TSC)-based metal complexes of Mn (II), Fe (II), and Ni (II) have been investigated. The synthesized metal complexes were characterized using elemental analysis, magnetic susceptibility, molar conductivity, FTIR, and UV-Vis spectroscopy, the computational path helped with further structural investigation. The solubility test on the TSC and its complexes revealed their solubility in most organic solvents.

High clinical burden of classical homocystinuria in the United States: a retrospective analysis.

Background: Classical homocystinuria (HCU) is a rare genetic metabolic disorder resulting in elevated homocysteine and methionine levels. The clinical characteristics and associated complications of HCU are well documented. However, there is limited published research on the clinical burden of patients with HCU, especially stratified by total homocysteine (tHcy) levels.

TNFSF9 Silence Impedes Cerebral Ischemia-Reperfusion Injury via Modulating SLC3A2 Expression in Brain Microvascular Endothelial Cells.

Cerebral ischemia-reperfusion injury (CIRI), which stays unresolved in the clinic, occurs after recanalization of blood vessels serving brain tissues in acute ischemic stroke patients and can result in massive brain cell death, and cell ferroptosis contributes greatly to this process. Our research firstly found that TNFSF9 expression harbored diagnostic value on CIRI patients and intended to further investigate its regulatory mechanism in CIRI, which might facilitate its diagnostic and therapeutic application in the clinic. The level of TNSF9 mRNA was augmented in the plasma of CIR patients, and its silence impeded ferroptosis, apoptosis, and release of inflammatory mediators of BMECs with OGD/R treatment.

Microwave-assisted selective leaching of valuable metals from spent lithium-ion batteries using glycine and oxalic acid.

Supplying critical metals such as cobalt, lithium, and nickel, to achieve sustainable development goals will be one of the most important concerns in the coming decades. A significant challenge in this area is the separation of some similar elements, such as nickel and cobalt. This study proposes a novel and eco-friendly process to selectively recover cobalt from nickel and manganese.

Examination of heavy metal concentrations and their interaction with anthropogenic sources in Ermenek Dam Lake (Turquoise Lake).

This study aims to determine the spatial distribution of heavy metal pollution in Ermenek Dam Lake, water quality assessment and pollution sources. For this purpose, samples were taken 6 times a year from 12 points determined in 2024. Physico-chemical parameters and heavy metals were analyzed in the study.

Suppressing Jahn-Teller distortion of MnO via B-Ni dual single-atoms integration for methane catalytic combustion.

Precisely managing electron transfer pathways throughout the catalytic reaction is paramount for bolstering both the efficacy and endurance of catalysts, offering a pivotal solution to addressing concerns surrounding host structure destabilization and cycling life degradation. This paper describes the integration of B-Ni dual single-atoms within MnO channels to serve as an electronic reservoir to direct the electron transfer route during methane catalytic combustion. Comprehensive analysis discovers that B atoms weaken the interaction between O and Mn atoms by forming bonds with lattice oxygen atoms.

Revealing ionically isolated Li loss in practical rechargeable Li metal pouch cells.

The degradation of rechargeable lithium (Li) metal batteries is primarily attributed to active Li loss, encompassing isolated Li, also known as "dead Li", and solid electrolyte interphase (SEI-Li). Comprehending the formation of dead Li is pivotal for devising strategies to mitigate Li loss. Herein, we reveal the existence of an alternative form of dead Li, termed ionically isolated Li (I-iLi), which diverges from the traditionally recognized electronically isolated Li (E-iLi).

Infection with the zoonotic nematode, Trichinella murrelli in bobcats (Lynx rufus) from Minnesota, USA.

During a survey for other parasitic infections, tongue and limb muscles of wild bobcats (Lynx rufus) were examined microscopically from a sample of 190 Minnesota bobcats. One batch (n = 50) was studied in 2015, yielding no cases of Trichinella when examined only with hematoxylin and eosin (HE) staining of <25 mg of tissue. In a 2024 cohort (n = 140), this method was paired with a compression of ∼5 g of tissue.

Distribution, sources, contamination, and risks of toxic metals in Zijiang River, a typical tributary of the midstream of the Yangtze River in China.

Excessive concentrations of toxic metals are a global threat to aquatic systems. Taking a typical tributary (Zijiang River, ZR) of the midstream of the Yangtze River as the research area, the concentration distribution and chemical fractions occurrence characteristics of five toxic metals (Cd, Cr, Cu, Pb, and Zn) were analyzed, their potential sources were explored, and their contamination and ecological risk was assessed. In the surface waters and sediments, there were high concentrations of Zn, a low concentration of Cd, and small spatial differences in concentration among the upstream, midstream, and downstream.

[Expression and enzymatic characterization of a chitosanase with tolerance to a wide range of pH from ].

To screen and identify a chitosanase with high stability, we cloned the chitosanase gene from with a high protease yield from the barren saline-alkali soil and expressed this gene in . The expressed chitosanase of . (BA-CSN) was purified by nickel-affinity column chromatography.

[A novel carbonyl reductase for the synthesis of ()-tolvaptan].

Screening carbonyl reductases with the ability to catalyze the reduction of complex carbonyl compounds is of great significance for the biosynthesis of -tolvaptan(-TVP). In this study, the target carbonyl reductase in the crude enzyme extract of rabbit liver was separated, purified, and identified by ammonium sulfate precipitation, gel-filtration chromatography, ion exchange chromatography, affinity chromatography, and protein mass spectrometry. With the rabbit liver genome as the template, the gene encoding the carbonyl reductase was amplified by PCR and the recombinant strain was successfully constructed.

Clinical outcomes of patients with unsuccessful mechanical thrombectomy versus best medical management of medium vessel occlusion stroke in the middle cerebral artery territory.

Background: Current randomized controlled trials are investigating the efficacy and safety of mechanical thrombectomy (MT) in patients with medium vessel occlusion (MeVO) stroke. Whether best medical management (MM) is more efficient than unsuccessful vessel recanalization during MT remains unknown.

Methods: This was a retrospective cohort study using data from 37 academic centers across North America, Asia, and Europe between September 2017 and July 2021.