X-ray irradiation as a potential postharvest treatment for maintaining the quality of lily (Lilium davidii var. unicolor) bulbs and predicting shelf life using an artificial neural network.

This study aimed to investigate the impact of X-ray irradiation pretreatment at varying doses (0.5, 1.0, 1.

Microbial lysates as low-cost serum replacements in cellular agriculture media formulation.

Cultivated meat, the process of generating meat in vitro without sacrificing animals, is a promising alternative to the traditional practice of livestock agriculture. However, the success of this field depends on finding sustainable and economical replacements for animal-derived and expensive fetal bovine serum (FBS) that is typically used in cell culture processes. Here, we outline an effective screening process to vet the suitability of microbial lysates to support the growth of immortalized bovine satellite cells (iBSCs) and mackerel (Mack1) cells.

An ammonia-responsive aerogel-type colorimetric sensor for non-destructive monitoring of shrimp freshness.

The colorimetric sensor for volatile amines (VA) detection can realize non-destructive monitoring of shrimp quality. However, its sensing performance still needs to be improved. In this study, we proposed an aerogel-type colorimetric sensor to improve VA sensing performance and realize early detection of shrimp spoilage.

Integrated biotechnological process based on submerged fermentation and sonotrode extraction as a valuable strategy to obtain phenolic enriched extracts from moringa leaves.

The present study explored the influence of submerged fermentation with different lactic acid bacteria (LAB) as a pre-treatment of moringa leaves before the extraction of phenolic compounds via sonotrode. The sonotrode extraction was optimized using a Box-Behnken design and the optimal conditions found were: 25 % ethanol, 5 min and 100 % amplitude. Folin-reacting substances and antioxidant activity were measured on hydroalcoholic extracts obtained from fermented moringa leaves and, individual phenolic compounds were determined by HPLC-ESI-TOF-MS for the first time.

Dynamics of microbial communities and metabolites during the fermentation of Ningxia goji berry wine: An integrated metagenomics and metabolomics approach.

Ningxia Goji Berry Wine (NGBW), a traditional Chinese fermented beverage, exhibits complex flavor quality changes during fermentation, the mechanisms of which remain insufficiently elucidated. This study aimed to elucidate the dynamic shifts in physicochemical properties, metabolites, and microbial communities throughout the controlled fermentation process of NGBW. Metabolomic analysis identified 8 key differential volatile metabolites (VOCs) and 406 differential non-volatile metabolites.

Strong, tough, antibacterial, antioxidant, biodegradable multi-functional intelligent hydrogel film for real-time detection and maintenance of salmon freshness.

In this study, we prepared a new multi-functional intelligent hydrogel preservation film using soy hull nanocellulose (SHNC), polyvinyl alcohol (PVA), chitosan (CS), and anthocyanin (Anth) as raw materials. The physicochemicals of the hydrogel preservation film, and its role in monitoring the freshness and freshness of salmon was evaluated. The results showed that the monomers were crosslinked by hydrogen, ester bonds, and electrostatic interactions in the hydrogel film, and there were three-dimensional pores in the hydrogel film.

Global Epidemiology and health risks of Bacillus cereus Infections: Special focus on infant foods.

Bacillus cereus is a common pathogen responsible for gastrointestinal and other complicated disorders, yet epidemiological data and public health measures remain scarce. To bridge these gaps, a bilingual search spaning 50 years identified 266 relevant studies on global B. cereus infection, encompassing 6,135 cases.

Xanthine oxidase-lactoperoxidase system: Dose-dependent antibacterial effects and global gene expression changes in infant oral microbiota.

Xanthine oxidase (XO) and lactoperoxidase (LPO) are highly abundant enzymes in milk. Their substrates, xanthine and thiocyanate, are found in elevated amounts in infant saliva, leading to a proposed interaction between milk and saliva referred to as the XO-LPO system. This system is suggested to generate reactive oxygen and nitrogen species with potential antibacterial effects.

Prevention and control strategies for psychrophilic Pseudomonas fluorescens in food: A review.

Psychrophilic Pseudomonas fluorescens can secrete extracellular enzymes, biofilms, and other substances even under refrigeration conditions, which have a negative impact on the quality of dairy products, aquatic products, meat products, produce, and other foods, causing food spoilage and huge economic losses. Therefore, strengthening the prevention and control of psychrophilic P. fluorescens in food is of great significance.

Engineering antibacterial plasma-polymerized polyethylene glycol-ZnO nanocomposite coatings for extending pork sausage shelf life.

Recently, interest in eco-friendly techniques for producing antibacterial food packaging films has surged. Within this context, plasma polymerization is emerging as a promising approach for applying degradable antibacterial coatings on various plastic films. This research therefore employs an atmospheric pressure aerosol-assisted plasma deposition technique to create polyethylene glycol (PEG)-like coatings embedding zinc oxide nanoparticles (ZnO NPs) of varying sizes on polyethylene (PE) substrates.

Deep learning enabled rapid classification of yeast species in food by imaging of yeast microcolonies.

Diverse species of yeasts are commonly associated with food and food production environments. The contamination of food products by spoilage yeasts poses significant challenges, leading to quality degradation and food loss. Similarly, the introduction of undesirable strains during fermentation can cause considerable challenges with the quality and progress of the fermentation process.

High pressure processing at different hydration levels as a tool to enhance rice bran stability and techno-functionality.

High-pressure processing (HPP) enhances food safety and shelf life by inactivating microorganisms and preserving food quality, yet its effectiveness in low-humidity environments has not been evaluated. This study investigated the effects of HPP at 500 MPa for 15 min across varying hydration levels (15, 30, 60, 77 %) on rice bran (RB), aiming to identify microbial effectiveness, besides techno-functional and physicochemical properties. HPP effectively reduced mesophilic bacteria, molds and yeast of RB at > 15 % hydration level, achieving reductions of up to 4 logarithmic cycles in the latter, nearing the detection limit of the method.

(E)-2-Octenal inhibits Neofusicoccum parvum growth by disrupting mitochondrial energy metabolism and is a potential preservative for postharvest mango.

Stem-end rot caused by Neofusicoccum parvum is among the most detrimental diseases affecting postharvest mangoes. The present investigation utilized (E)-2-octenal to manage N. parvum infections, elucidating its mechanism of action.

Changes in functional activities and volatile flavor compounds of fermented mung beans, cowpeas, and quinoa started with Bacillus amyloliquefaciens SY07.

In this work, the functional activities including α-glucosidase, α-amylase, angiotensin converting enzyme (ACE) inhibitory activity, and antioxidant activity of mixed grains (mung beans, cowpeas, and quinoa) fermented with Bacillus amyloliquefaciens SY07 were investigated. The volatile flavor of the mixed grains collected every 12 h during 72 h-fermentation were further detected as well. The inhibition on α-glucosidase and α-amylase reached up to 89.

Microencapsulated phenolic compounds from organic coffee husk: Impacts on human gut microbiota and in vitro prebiotic potential.

This research investigated the influence of the microencapsulation of phenolic compounds (PCs) from organic coffee husk with whey protein concentrate (WPC) and maltodextrin on the abundance of intestinal bacterial populations and their metabolic activity during in vitro fecal fermentation. The microencapsulated PCs were gradually metabolized during fecal fermentation, resulting in significant transformations and an increase in PCs in the fermentation media. The metabolism of PCs by the fecal microbiota occurred concurrently with the consumption of sugars, production of organic acids, and reduction in pH in the media.

Multi-Omics analysis reveals the sensory quality and fungal communities of Tibetan teas produced by wet- and dry-piling fermentation.

Ya'an Tibetan tea, a dark tea with a rich historical heritage, is typically processed using two primary piling fermentation methods: wet piling with rolled leaves (moisture content around 60%) and dry piling with sun-dried or baked green tea leaves (moisture content below 30%). This study employed sensory evaluation, targeted and non-targeted metabolomics, and fungal Internal Transcribed Spacer (ITS) sequencing to investigate changes in quality components and fungal composition in Tibetan tea processed by both wet and dry-piling methods. The results revealed that 3,7-Dimethyl-1,5,7-octatriene-3-ol and D-limonene were identified as key volatile metabolites contributing to the aroma variations between the dry and wet-piled teas.

Modeling the growth and dynamics of uropathogenic Escherichia coli in sugarcane juice for shelf life predictions.

In this study, we assessed the effects of temperature and dilution on uropathogenic Escherichia coli (UPEC) growth in sugarcane juice and modeled the kinetics for shelf life simulation. Diluted and undiluted sugarcane juice samples inoculated with a four-strain UPEC cocktail were stored at 4, 10, 15, 20, 30, and 40 °C to evaluate their growth during storage. Changes in UPEC growth were fitted using three primary models (Baranyi, Huang, and reparameterized Gompertz models), and two secondary models (Huang square-root and Ratkowsky square-root models) were selected to evaluate the effect of temperature on specific growth rates.

Ergothioneine exerts neuroprotective effects in Parkinson's disease: Targeting α-synuclein aggregation and oxidative stress.

Ergothioneine (EGT) is a natural dietary antioxidant derived from certain edible mushrooms, commonly used as a food additive and supplement, but its effects on Parkinson's Disease (PD) are still unclear. The accumulation of α-synuclein (α-syn) plays a pivotal role in the pathogenesis and development of PD. Here, this study demonstrated that EGT effectively inhibits α-syn aggregation, disrupts mature fibers, and reduces associated cytotoxicity and oxidative stress.

Nanoencapsulation reduces the perception of carvacrol odor, enhances the control of Botrytis cinerea growth and preserves grape quality.

Botrytis cinerea is the causal agent of gray mold, which is one of the most widespread and destructive fungal diseases that compromises the productivity and quality of grapes produced throughout the world. This work aimed to verify, for the first time, the impact of unencapsulated carvacrol and encapsulated in Eudragit® nanocapsules (Eud-Carv NCs) and chia mucilage (Chia-Carv NCs) on mycelial growth and spore germination of B. cinerea.

Modulation of microbiota composition and markers of gut health after in vitro dynamic colonic fermentation of plant sterol-enriched wholemeal rye bread.

A human oral phase followed by a dynamic gastrointestinal digestion and colonic fermentation (simgi®) has been applied to wholemeal rye bread (WRB) and PS-enriched WRB (PS-WRB). The aim of this study was to evaluate the impact of these solid and high-fiber food matrices on the metabolism of PS, modulation of the microbiota and production of short-chain fatty acids (SCFA) and ammonium ion after a simulated chronic intake (5 days). In both breads, campesterol, campestanol, stigmasterol, β-sitosterol, sitostanol, Δ5-avenasterol, Δ5,24-stigmastadienol, Δ7-stigmastenol, and Δ7-avenasterol were identified, of which only β-sitosterol was metabolized to sitostenone after PS-WRB treatment.

Screening of strains from pickles and evaluation of characteristics of different methods of fast and low salt fermented mustard leaves (Brassica juncea var. multiceps).

The aim of this study was to isolate strains with excellent fermentation performance from pickles, thus enhancing the quality of rapid, low-salt fermented mustard leaves (Brassica juncea var. multiceps) through process optimization and inoculation fermentation. A high-throughput screening method for acid-producing strains was developed, significantly improving screening efficiency.

Fermentation with Lactobacillus strains, Acetobacter pasteurianus, and Torulaspora delbrueckii D1-3 improves nutritional quality and volatile profile of sea buckthorn-based cereal beverage.

Recently, there has been a growing demand for plant-based beverages that meet nutritional and health needs and have an appealing taste. This study investigated the impact of fermentation with Lactobacillus strains, Acetobacter pasteurianus, and Torulaspora delbrueckii D1-3 on the nutritional quality and aroma compound profile of a sea buckthorn-based cereal beverage. The mixed starter fermented samples, specifically S-APTD (SBCB inoculated with A.

Long-term effects of Nε-carboxymethyllysine intake on intestinal barrier permeability: Associations with gut microbiota and bile acids.

Advanced glycation end products (AGEs) in processed foods are closely linked to intestinal injury. However, the long-term effects of exposure to free Nɛ-carboxymethyl lysine (CML), a prevalent AGE molecule, on intestinal barrier integrity have been rarely evaluated. This study investigated the temporal effects of CML exposure on intestinal barrier permeability in C57BL/6N mice at diet-related doses over 12, 14, and 16 weeks.

Non-targeted metabonomics reveals the effect of linalyl alcohol on Brochothrix thermophile and its potential application.

Brochothrix thermophcta (B. thermophcta) is a pathogenic microorganism associated with food contamination. Linalyl alcohol, owing to its broad spectrum and exceptional antibacterial properties, is regarded as a potent natural antimicrobial agent.

Molecular characterization of exopolysaccharide from Periweissella beninensis LMG 25373 and technological properties in plant-based food production.

Periweissella beninensis LMG 25373, belonging to the recently established Periweissella genus, exhibits unique motility and high adhesion capabilities, indicating significant probiotic potential, including resilience under simulated gastrointestinal conditions. This study demonstrates for the first time that P. beninensis LMG 25373^T produces a dextran-type exopolysaccharide (EPS) with a distinctive high degree of branching (approximately 71 % of α-(1 → 6)-linkages and 29 % α-(1 → 3)-linkages).