Identification of f.a., sp. nov. and large indels in the rRNA cistron that split the genus.

Yeast strains representing a novel asexual ascomycetous species were isolated from seven flowers. Sequencing of the chromosomal regions coding for the D1/D2 domains of the large subunit ribosomal RNA, the ITS1-5.8S-ITS2 segments and parts of the gene coding for the small subunit ribosomal RNA showed that the isolates were conspecific.

Exploring endophytic fungi from Cynodon dactylon: GC-MS profiling and biological activity.

The rapid decline of significant plant species due to deforestation and slow regrowth has endangered many trees that are crucial for producing life-saving medications. This dual crisis of conserving plant biodiversity while meeting pharmaceutical demands necessitates innovative solutions. Endophytic fungi, naturally occurring symbionts within plants, present an eco-friendly and economically viable alternative.

Insights into , a Large-sized, Phylogenetically Unique Type Ia Methanotroph with Biotechnological Potential.

The cultivation of microorganisms is important as it provides us an opportunity to explore the characteristics that can be used for a variety of applications. Methanotrophs oxidize methane and are one of the most challenging organisms to culture. In 2018, we cultured one of the largest methanotrophs within the gammaproteobacterial group (Type Ia), a novel genus and species, , with large peculiar, elongated oval (cucumber-shaped) cells (~ 3-6 µm length × 1.

Discovery of : A Novel Fungus Identified Using Genome Sequencing and Metabolomic Analysis.

In this study, a new species of was isolated as an endophyte from the bark of from Mulshi, Maharashtra. The identity of this isolate was confirmed based on the asexual morphological characteristics as well as multi-gene phylogeny based on the internal transcribed spacer (ITS) and large subunit (LSU) nuclear ribosomal RNA (rRNA) regions. As this was the second species to be reported in this genus, we sequenced the genome of this species to increase our knowledge about the possible applicability of this genus to various industries.

The Genus : Insights on Taxonomy, Diversity and Distribution.

is the third largest rust genus of the order with more than 200 described species. It is an important rust genus that has undergone tremendous taxonomic changes. This genus produces teliospores united into a head on a compound pedicel composed of two to several hyphae with autoecious, macro-, demi- to hemi-, and, more rarely, microcyclic modes of their life cycle which provide it a unique identity and have proved helpful in the identification of the genus.

Strain-Dependent Adhesion Variations of Isolated from Healthy Human Volunteers: A Study on Cell Surface Properties and Potential Probiotic Benefits.

The probiotic potential of is widely recognized, but little is known about its adhesive properties. Hence, this study aims to investigate the adhesion potential and cell surface properties of four human-origin strains (B619/R, B603/Nb, B106, and B637/Nm). We evaluated epithelial adhesion, Extracellular Matrix (ECM) binding, aggregation ability, and cell surface hydrophobicity and used genome analysis for validation.

Role of grass endophytic fungi as a natural resource of bioactive metabolites.

Grass endophytic fungi have garnered increasing attention as a prolific source of bioactive metabolites with potential application across various fields, including pharmaceticals agriculture and industry. This review paper aims to synthesize knowledge on the diversity, isolation, and bioactivity of metabolites produced by grass endophytic fungi. Additionally, this approach aids in the conservation of rare and endangered plant species.

Monitoring Autophagy During Drosophila Oogenesis.

Macroautophagy (autophagy hereafter) is an evolutionarily conserved mechanism that maintains the health of cells by degrading toxic proteins and damaged organelles within the lysosomes. Tissues like ovary are made up of heterogeneous cell types and each cell type has distinct levels of autophagy. Studying autophagy in a cell-type specific manner helps better understand the role of autophagy during oogenesis.

Antioxidant and Antifungal Properties of Cinnamon, Cloves, L. and L. Extracts against Isolated from Infected Vegetables in Mauritius.

Background: species, a group of economically destructive phytopathogens, are poorly studied in Mauritius where agriculture holds much significance. Furthermore, the increasing popularity of organic farming has prompted interest in alternatives to chemical fungicides.

Methods: After gaining an overview of prevalence in Mauritius fields through a survey, the pathogen was isolated from infected crops and identified based on morphological and molecular characteristics.

TGF-β signaling molecules in : role of BMP and BMP inhibitors during pattern formation.

Understanding the evolution of body plans has been one of the major areas of investigation in developmental and evolutionary biology. Cnidaria, the sister group to bilaterians, provides an opportunity to elucidate the origin and evolution of body axes. , a freshwater cnidarian, is a useful model to study signaling pathways governing pattern formation, which are conserved up to vertebrates including humans.

Identification and characterization of multidomain monothiol glutaredoxin 3 from diploblastic Hydra.

Intracellular antioxidant glutaredoxin controls cell proliferation and survival. Based on the active site, structure, and conserved domain motifs, it is classified into two classes. Class I contains dithiol Grxs with two cysteines in the consensus active site sequence CXXC, while class II has monothiol Grxs with one cysteine residue in the active site.

Fungal Planet description sheets: 1478-1549.

Novel species of fungi described in this study include those from various countries as follows: , on whitefly, on bark of , from soil under , on leaf spot of , and on leaf spot of . , on fully submersed siliceous schist in high-mountain streams, and on the lower part and apothecial discs of on a twig. , on decaying wood, from moist soil with leaf litter, on a trunk of a living unknown hardwood tree species, and on dead twigs of unidentified plant.

Unveiling the Probiotic Potential of MCC0200: Insights from In Vitro Studies Corroborated with Genome Analysis.

is widely used as a starter culture in the dairy industry and has garnered attention as a beneficial bacterium owing to its health-promoting functionalities in humans. In this study, the probiotic potential of MCC0200 isolated from a dairy product was investigated through a combinatorial approach of in vitro and in silico studies. MCC0200 demonstrated the ability to survive harsh gastrointestinal (GI) transit, adhere to intestinal mucosa and exert health-promoting traits in in vitro studies.

Embryonic spatiotemporal expression pattern of Folded gastrulation suggests roles in multiple morphogenetic events and regulation by AbdA.

In Drosophila, the signaling pathway activated by the ligand Folded gastrulation (Fog) is among the few known G protein-coupled receptor (GPCR) pathways to regulate cell shape change with a well-characterized role in gastrulation. However, an understanding of the spectrum of morphogenetic events regulated by Fog signaling is still lacking. Here, we present an analysis of the expression pattern and regulation of fog using a genome-engineered Fog::sfGFP line.

Insights into the genomic architecture of a newly discovered endophytic species belonging to the complex from India.

In this study, a new species belonging to the species complex is established to accommodate an endophytic fungus isolated from sp. and collected from Himachal Pradesh. The identity of this isolate was confirmed based on the asexual morphs, its cultural characteristics, and phylogenetic analyses.

Enhanced production of cordycepin under solid-state fermentation of Cordyceps militaris by using combinations of grains/substrates.

This manuscript deals with cordycepin, an interesting secondary compound produced from entomopathogenic fungus, Cordyceps. It has attracted commercial interest due to its immense pharmacological importance beneficial to human health. In this study, the contents of cordycepin and its derivatives, like adenine and adenosine, were evaluated through solid-state fermentation using combinations of various grains as substrate.

The Enigmatic World of Fungal Melanin: A Comprehensive Review.

Synthetic dyes are generally not safe for human health or the environment, leading to the continuous search and growing demand for natural pigments that are considered safer, biodegrade more easily, and are environmentally beneficial. Among micro-organisms, fungi represent an emerging source of pigments due to their many benefits; therefore, they are readily viable on an industrial scale. Among all the bioactive pigments produced by fungi, melanin is an enigmatic, multifunctional pigment that has been studied for more than 150 years.

Diverse type I and type II methanotrophs cultivated from an Indian freshwater wetland habitat.

Wetlands are the main natural sources of methane emissions, which make up a significant portion of greenhouse gas emissions. Such wetland patches serve as rich habitats for aerobic methanotrophs. Limited knowledge of methanotrophs from tropical wetlands widens the scope of study from these habitats.

f.a., sp. nov., a new yeast species isolated from the gut of the wood-feeding termite sp.

The gut of xylophagous insects such as termites harbours various symbiotic micro-organisms, including many yeast species. In a taxonomic study of gut-associated yeasts, two strains (ATS2.16 and ATS2.

Fungal alkaline proteases and their potential applications in different industries.

The consumption of various enzymes in industrial applications around the world has increased immensely. Nowadays, industries are more focused on incorporating microbial enzymes in multiple processes to avoid the hazardous effects of chemicals. Among these commercially exploited enzymes, proteases are the most abundantly used enzymes in different industries.

Thermotolerant methanotrophs belonging to the Methylocaldum genus dominate the methanotroph communities in biogas slurry and cattle dung: A culture-based study from India.

Biogas reactors run on various types of waste, with cattle dung and agricultural wastes being the primary sources in India. As biogas contains 50-60% methane, there is a possibility that the reactors harbour methanotrophs or methane-oxidizing bacteria. We set up serial endpoint dilution enrichments for the cultivation of methanotrophs using slurry from a small biogas reactor and cattle dung samples and obtained cultures of Methylocaldum gracile, a thermotolerant methanotroph.