Synthesis, Characterization, Antitubercular Activity, and Molecular Docking Studies of Pyrazolylpyrazoline-Clubbed Triazole and Tetrazole Hybrids.

To increase the antitubercular potency, we synthesized a series of novel pyrazolylpyrazoline derivatives (-) using the one-pot multicomponent reaction of the substituted heteroaryl aldehyde (,), 2-acetyl pyrrole/thiazole (,), and substituted hydrazine hydrates (-) in the presence of base NaOH as a catalyst in ethanol as the solvent at room temperature. Substituted heteroaryl aldehyde (,) was synthesized from 5-chloro-3-methyl-1-phenyl-1-pyrazole-4-methyl-carbaldehyde on protection with ethylene glycol followed by treatment with 4-amino triazole/5-amino tetrazole and then deprotection using acid. The salient features of the green protocol are the one-pot reaction, shorter reaction time, and straightforward workup procedure.

Petroleum sludge polluted soil remediation: Integrated approach involving novel bacterial consortium and nutrient application.

Petroleum sludge has been reported as noteworthy hazardous solid waste generated from industrial activities of petroleum sector. Environment friendly and economically sound treatment of petroleum sludge has attracted global attention worldwide and has become a thrust area of research. Petroleum sludge bioremediation is gaining interest of researchers globally to clean pollutants from soil ecosystems.

Bioaugmentation of Pseudomonas aeruginosa NCIM 5514 - A novel oily waste degrader for treatment of petroleum hydrocarbons.

This study was aimed at remediation ofoily waste contaminated area by utilizing a newly obtained bacterium. For experimental setup three different approachessuch as bioaugmentation, natural attenuation and abiotic factors were employed. In bioaugmented experimental set up (treatment withP.

Oilfield waste treatment using novel hydrocarbon utilizing bacterial consortium - A microcosm approach.

Oily sludge is a hazardous waste generated through petroleum producing and processing industrial units. Due to its harmful environmental impacts, it needs to be treated in sustainable manner. The present study aimed to evaluate influence of bioaugmentation on oily sludge biodegradation efficiency of a novel hydrocarbon utilizing bacterial consortium (HUBC) using microcosms.

Bioremediation of oily sludge polluted soil employing a novel strain of Pseudomonas aeruginosa and phytotoxicity of petroleum hydrocarbons for seed germination.

Agricultural land pollution is key a problem globally, which is linked with growth of industries. Petroleum industrial sector is one of the major industrial sectors and the activities of petroleum industry lead to the agricultural land pollution. Oily sludge is a type of solid and hazardous waste generated from petroleum industrial activities.

Comparing Bioremediation Approaches for Agricultural Soil Affected with Petroleum Crude: A Case Study.

The aim of work was to check and make comparison of efficacy for five approaches for petroleum crude contaminated agricultural soil remediation by making use of soil microcosms. Concerning the published literature in our information, this is the first report comparing five approaches i.e.

Evaluation of rhamnolipid production by a halotolerant novel strain of Pseudomonas aeruginosa.

This work was aimed to evaluate six qualitative and quantitative methods (hemolytic activity, cetyltrimethylammonium bromide agar plate method, oil spread method, drop collapse method, surface tension measurement and emulsification index) to study biosurfactant production by sixty-nine bacterial isolates which were obtained from petroleum crude contaminated soil and water samples. Among all isolates, Pseudomonas aeruginosa NCIM 5514 was evaluated as the most potent isolate producing rhamnolipid. Effectiveness of growth medium pH, inoculum size and concentration of NaCl on surface active properties and biomass & rhamnolipid production in fermentation medium were studied.

Influence of abiotic factors, natural attenuation, bioaugmentation and nutrient supplementation on bioremediation of petroleum crude contaminated agricultural soil.

Contamination of agricultural land(s) is a major problem worldwide which is associated with activities of petroleum industry. Due to these exploration activities remedial techniques for clean-up of contaminated agricultural soil(s) has become an alarming research topic. Hydrocarbon utilizing bacterial consortium (HUBC), isolated from petroleum crude (petroleum industry waste water and soil) contaminated sites, India has been used for soil microcosm study.

Geological evidence for AD 1008 tsunami along the Kachchh coast, Western India: Implications for hazard along the Makran Subduction Zone.

The 2004 Sumatra-Andaman tsunami emphasized the catastrophic nature of such disasters and exposed our knowledge gap of the historical and palaeo events. In the aftermath of this deadly event, the thrust in palaeotsunami studies was restricted to areas in the Indian Ocean, affected by this tsunami. The northern Arabian Sea, which hosts a similar tsunamigenic source i.

-(6-Meth-oxy-pyridin-2-yl)-1-(pyridin-2-ylmeth-yl)-1-pyrazole-3-carboxamide: crystal structure and Hirshfeld surface analysis.

The title compound, CHNO, adopts the shape of the letter with the dihedral angle between the outer pyridyl rings being 78.37 (5)°; the dihedral angles between the central pyrazolyl ring (r.m.

Ethyl 2-amino-1-(4-fluoro-phen-yl)-5-oxo-4,5-di-hydro-1-pyrrole-3-carboxyl-ate: crystal structure and Hirshfeld surface analysis.

In the title mol-ecule, CHFNO, the central pyrrole ring makes a dihedral angle of 9.2 (3)° with the eth-oxy carbonyl moiety whereas the fluoro-phenyl ring is rotated by 67.6 (2)° from the pyrrole ring.

Critical review on biosurfactant analysis, purification and characterization using rhamnolipid as a model biosurfactant.

Surfactants are one of the most versatile group of chemicals used in various industrial processes. Their market is competitive, and manufacturers will have to expand surfactant production in ecofriendly and cost effective manner. Increasing interest in biosurfactants led to an intense research for environment friendly and cost-efficient production of biosurfactant.

Biodegradation of petroleum hydrocarbons by oleophilic strain of Pseudomonas aeruginosa NCIM 5514.

The aim of this work was to study the potential of an indigenous strain of Pseudomonas aeruginosa NCIM 5514, isolated from petroleum-polluted soil, for the biodegradation of crude petroleum oil. The isolate completely decolorized 2,6-dichlorophenol indophenol in 120h when grown at (37±1°C), indicating its hydrocarbon utilizing nature. Ex situ biodegradation study was performed to find out quantitative utilization and biodegradation of paraffin(s) present in crude oil.

Carbon spectrum utilization by an indigenous strain of Pseudomonas aeruginosa NCIM 5514: Production, characterization and surface active properties of biosurfactant.

The present research work was undertaken with a mandate to study carbon spectrum utilization and structural characterization of biosurfactant produced by indigenous Pseudomonas aeruginosa NCIM 5514, which showed unique properties to utilize a large number of carbon sources effectively for production of biosurfactant, although glucose was the best carbon substrate. In Bushnell-Hass medium supplemented with glucose (1%, w/v), 3.178±0.

Core Flood study for enhanced oil recovery through ex-situ bioaugmentation with thermo- and halo-tolerant rhamnolipid produced by Pseudomonas aeruginosa NCIM 5514.

The aim of this work was to study the Microbial Enhanced Oil Recovery (MEOR) employing core field model ex-situ bioaugmenting a thermo- and halo-tolerant rhamnolipid produced by Pseudomonas aeruginosa. Thin Layer Chromatography (TLC) revealed that the biosurfactant produced was rhamnolipid type. Nuclear Magnetic Resonance analysis showed that the purified rhamnolipids comprised two principal rhamnolipid homologues, i.

Application of UPLC-MS/MS for separation and quantification of 3α-Hydroxy Tibolone and comparative bioavailability of two Tibolone formulations in healthy volunteers.

A novel, fast, sensitive and robust method based on ultra-performance liquid chromatography coupled to atmospheric pressure electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) has been developed to separate two Tibolone stereoisomers i.e., 3α-Hydroxy Tibolone and 3β-Hydroxy Tibolone and to quantify 3α-Hydroxy Tibolone using p-toulenesulfonyl isocyanate (PTSI) as a derivatizing reagent in human plasma.