Computational Analysis and Synthesis of Syringic Acid Derivatives as Xanthine Oxidase Inhibitors.

Background: Xanthine oxidase (XO; EC 1.17.3.

Lithium potentiated, pyridoxine abolished and fluoxetine attenuated the anxiolytic effect of diazepam in mice.

In the present study, the anxiolytic effect of diazepam (1 and 2 mg/kg, i.p.) was determined alone and in combination with lithium (50 mg/kg, i.

Diazenyl Derivatives and their Complexes as Anticancer Agents.

In the past years, many diazenyl compounds (i.e diazenecarboxamides, diazeniumdiolate prodrugs, diazenyl complexes etc.) have been prepared for the evaluation of their cytotoxic potential towards various cancer cell lines.

Diazenyl derivatives as therapeutic and diagnostic agents acting on central nervous system.

In last decade, the development of new drugs and drug products for central nervous system (CNS) has remained limited due to incomplete elucidation of pathophysiology of many CNS disorders, complexity of the diseases and the lack of technologies for delivery through the blood-brain barrier (BBB). In this article we will summarize the development of diazenyl derivatives of many biologically active moieties like benzodiazepine, formazan, indole, pyrimidine, thiazole, sulfonamide etc. as diagnostic and therapeutic agents for CNS.

Structural and metabolic correlation for Bacillus megaterium ACBT03 in response to colchicine biotransformation.

This study aims to evaluate the effects of colchicine on metabolic and structural changes in Bacillus megaterium ACBT03, enduring colchicine bioconversion. Electron microscopy examination of cells adapted to different concentrations of colchicine for its bioconversion to pharmacologically active 3-demethylated colchicine, endowed changes in cell shape, decreased cell wall and plasma membrane thickness. In line with microscopic studies, lipid and membrane protein contents were drastically reduced in bacterial cells adapted to higher concentrations of colchicine and resulting into decrease in cell membrane thickness.

Statistical optimization of process variables for the production of an anticancer drug (colchicine derivatives) through fermentation: at scale-up level.

Effect of various nutritional components and fermentation parameters on biotransformation of colchicine using Bacillus megaterium ACBT03 to produce 3-demethylated colchicine (3-DMC) has been investigated. Initially, Plackett-Burman (PB) design was used to evaluate the 11 nutritional variables to screen medium component that significantly influences the 3-demethylated colchicine (3-DMC) production. Three most statistically significant nutrients that is starch, glycerol and yeast extract were identified.