1 results match your criteria: "Linz and Elisabethinen Hospital[Affiliation]"

Immunodetection array.

Methods Mol Biol

March 2009

Red Cross Transfusion Service of Upper Austria, Linz and Elisabethinen Hospital, 1st Department of Internal Medicine, Linz, Austria.

A novel procedure for DNA methylation analysis is described that characterizes the extent of DNA methylation in CpG islands. The basic concept relies on direct immunodetection of 5' methylcytosines (5' mCs) without the need for bisulfite treatment utilizing a microarray format. This system is designed for the application of immunofluorescence using a monoclonal antibody that specifically recognizes 5' mC in single-stranded DNA hybridized to oligonucleotide microarrays.

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