[Mechanism of astragaloside Ⅳ alleviating PC12 cell injury by activating PI3K/AKT signaling pathway: based on network pharmacology and in vitro experiments].

In this study, the molecular mechanism of astragaloside Ⅳ(AS-Ⅳ) in the treatment of Parkinson's disease(PD) was explored based on network pharmacology, and the potential value of AS-Ⅳ in alleviating neuronal injury in PD by activating the PI3 K/AKT signaling pathway was verified through molecular docking and in vitro experiments. Such databases as SwissTargetPrediction, BTMAN-TAM, and GeneCards were used to predict the targets of AS-Ⅳ for the treatment of PD. The Search Tool for the Retrieval of Interacting Genes/Proteins(STRING) was employed to analyze protein-protein interaction(PPI) and construct a PPI network, and the Database for Annotation, Visualization and Integrated Discovery(DAVID) was used for Gene Ontology(GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis.

Study on parental satisfaction and clinical treatment outcomes of 128 diarrheic children receiving comprehensive nursing.

Objective: To explore the clinical treatment outcomes and parental satisfaction of children with diarrhea receiving comprehensive nursing intervention.

Methods: A total of 128 diarrheic children treated in our hospital from June 2016 to June 2017 were recruited and divided into a control group (n=64) receiving conventional nursing and an observation group (n=64) receiving comprehensive nursing intervention, as per a random number table. The clinical outcomes, electrolyte disorders, recovery of gastrointestinal function, quality of life and parental satisfaction were compared between the two groups after nursing.

The application value of informatization-based extended nursing care on discharged children with leukemia.

Objectives: This study explored and analyzed the application value of informatization-based extended care on children discharged with leukemia.

Methods: 109 children receiving chemotherapy for acute leukemia in our hospital from January 2018 to January 2020 were selected as research subjects. The children were divided into the control group (n=53) and the observation group (n=56) on the basis of admission time point.

The diagnostic value of 3.0T MRI in cesarean scar pregnancy.

Objective: The purpose was to evaluate the diagnostic value of 3.0T MRI in cesarean scar pregnancy (CSP).

Methods: 56 patients with suspected CSP treated in our hospital from August 2018 to July 2020 were recruited as the study cohort and diagnosed using ultrasound and 3.

Aerosol inhalation of ambroxol hydrochloride combined with terbutaline can promote recovery of children with severe pneumonia.

Objective: This research aimed to investigate the clinical efficacy of aerosol inhalation of ambroxol hydrochloride combined with terbutaline on children with severe pneumonia, and to evaluate its influence on their immune function and inflammatory level.

Methods: Totally 113 severe pneumonia children were included. Thereinto, 55 children in the control group (CG) were treated with terbutaline aerosol inhalation, while 58 in the research group (RG) were given ambroxol hydrochloride on the basis of the CG.

Smad7 suppresses melanoma lung metastasis by impairing Tregs migration to the tumor microenvironment.

Transforming growth factor β (TGF-β) signaling plays critical roles in both physiological and pathological conditions. In the tumor microenvironment, TGF-β are well demonstrated as a tumor inducer, which also promote tumor growth and metastasis. SMAD family is an important TGF-β signalling transducer, which consists of receptor-regulated SMADs (R-SMADs), common-mediator SMADs (co-SMADs), and inhibitory SMADs (I-SMADs).

An easy synthesis of nitrogen and phosphorus co-doped carbon dots as a probe for chloramphenicol.

Heteroatom doping in carbon dots (CDs) was found to be an efficient way to regulate the structure of electronic energy levels and enhance the fluorescence characteristics of CDs. Nevertheless, most reported fabrication processes of heteroatom-doped CDs are rigorous and complex. Herein, a facile and novel strategy was developed to rapidly prepare nitrogen and phosphorus co-doped CDs (N,P-CDs) using acetic acid as the carbon source, and arginine, 1,2-ethylenediamine (EDA) and diphosphorus pentoxide as the dopants, respectively.

LncRNA meg3 suppresses hepatocellular carcinoma in vitro and vivo studies.

Although abnormal expression of the long non-coding RNA (lncRNA) MEG3 has been reported in multiple cancer types, the role of MEG3 in the pathobiology of hepatocellular carcinoma (HCC) remains unknown. This study evaluated the expression of lncRNA MEG3 and a microRNA (miRNA-10a-5p) implicated in HCC metastasis in cancer and carcinoma adjacent tissues of HCC samples (n=30 each) via in situ hybridization and quantitative RT-PCR. The effects of overexpressing either MEG3 alone, or MEG3 in combination with miRNA-10a-5p, on the proliferation, apoptosis, cell cycle progression, migration, and invasion of HepG2 cells were evaluated using functional assays.

Decreased expression of long non-coding RNA SNHG7 cause recurrent spontaneous abortion through suppression proliferation and invasion of trophoblast cells via miR-34a.

Little is known about the role of long non-coding RNA SNHG7-1 in the development of recurrent spontaneous abortion (RSA). The aim of the present study was to investigate the levels of SNHG7-1 in villi of RSA, and explore its underlying mechanism. The qRT-PCR assay SNHG7-1 showed that the expression level was downregulated in RSA and HTR-8/SVneo cells.