SNHG3 silencing suppresses the malignant development of triple-negative breast cancer cells by regulating miRNA-326/integrin α5 axis and inactivating Vav2/Rac1 signaling pathway.

Objective: Long non-coding RNA small nucleolar RNA host gene 3 (SNHG3) has been shown to participate in several tumorigenesis. Triple-negative breast cancer (TNBC) is an aggressive type of breast cancer, which is the first leading cause of new cancer diagnoses in women globally. However, the role of SNHG3 remains little known in breast cancers, especially in TNBC.

Long non-coding RNA OR3A4 facilitates cell proliferation and migration in colorectal cancer through the Wnt/β-catenin signaling pathway.

Objective: Colorectal cancer (CRC) remains one of the most ordinary cancers worldwide. Recently, researches have suggested the important role of long noncoding RNAs (lncRNAs) in the progression of tumorigenesis. This study aims to identify how lncRNA OR3A4 functions in the development of CRC.

LINC00473 functions as an oncogene and predicts poor prognosis in pancreatic cancer via the cAMP/β-catenin axis.

Objective: To investigate the expression and function of LINC00463 in pancreatic cancer (PC), and to demonstrate the relationship between LINC00473 expression and clinical pathological characteristics and prognosis of PC.

Patients And Methods: Expressions of LINC00473 in PC tissues and cell lines were detected using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). LINC00473 siRNA was synthesized to knock down the LINC00473 expression in PANC-1 cells.

Serum lncRNA LOXL1-AS1 is a diagnostic and prognostic marker for epithelial ovarian cancer.

Background: The present study aimed to examine the levels of circulating LOXL1-AS1 in epithelial ovarian cancer (EOC) patients and to analyze its diagnostic and prognostic value.

Methods: The levels of LOXL1-AS1 in 185 EOC patients and 43 healthy volunteers were evaluated by a quantitative reverse transcriptase-polymerase chain reaction. The potential of LOXL1-AS1 as a biomarker for EOC diagnosis was determined by receiver-operating characteristic (ROC) curve assays.

Preparation of Programmed Death-Ligand 1 Antibody Nanoparticles and Their Lung Cancer Targeting Therapeutic Effects.

The antibody nanoparticles of programmed death-ligand 1 (PD-L1) were prepared and their characterization, drug loading ability, and targeting effect were evaluated. In addition, adriamycin-loaded lipid polymer nanoparticles (hereafter referred to as nanoparticles) were synthesized by a double emulsion method and thin film dispersion method and were coupled with PD-L1 antibody nanoparticles. The cytotoxicity of nanoparticles to A549 cells was detected , and the uptake of nanoparticles was detected by flow cytometry and confocal microscopy.

circ_103809 promotes breast cancer progression by regulating the PI3K/AKT signaling pathway.

Breast cancer is one of the most common cancer types in the world. This study was carried out to investigate the functional role of circular RNA circ_103809 in breast cancer. The expression of hsa_circ_103809 in breast cancer tissues and breast cancer cells were verified.

METTL3 promotes the progression of nasopharyngeal carcinoma through mediating M6A modification of EZH2.

Objective: The aim of this study was to investigate whether METTL3 promoted the progression of nasopharyngeal carcinoma (NPC) by silencing CDKN1C through EZH2.

Patients And Methods: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to examine the expression level of METTL3 in 48 pairs of NPC tissues and adjacent normal tissues. METTL3 expression in patients with different tumor lymph node metastasis (TNM) stages was detected by qRT-PCR as well.

MiR-142-5p reverses the resistance to gefitinib through targeting HOXD8 in lung cancer cells.

Objective: To investigate the role and potential mechanism of micro ribonucleic acid (miR)-142-5p in the acquired resistance to gefitinib in lung cancer cells.

Materials And Methods: The drug resistance of PC9/G cells was detected via methyl thiazolyl tetrazolium (MTT) assay. Expression levels of miR-142-5p and HOXD8 in PC9 and PC9/G cells were detected via quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) and Western blotting.

LINC01857 promotes the development of gastric cancer by regulating microRNA-200b.

Objective: The aim of this study was to investigate the expression characteristics of LINC01857 in gastric cancer (GCa), and to further study whether it could promote GCa development by modulating microRNA-200b.

Patients And Methods: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was used to examine LINC01857 expression in 60 pairs of GCa tissues and adjacent tissues. The interplay between LINC01857 level and clinical indexes and the prognosis of GCa patients was analyzed.

Propofol inhibits migration and induces apoptosis of pancreatic cancer PANC-1 cells through miR-34a-mediated E-cadherin and LOC285194 signals.

Propofol has exhibited potent antitumor activity in pancreatic cancer cells and . The study aimed to investigate the anti-tumor mechanisms of propofol on pancreatic cancer PANC-1 cells . PANC-1 cells were exposure to concentration 20 μg/ml of propofol for 72 h.

Silencing of Long Non-Coding RNA-HCG18 Inhibits the Tumorigenesis of Gastric Cancer Through Blocking PI3K/Akt Pathway.

Purpose: Long non-coding RNAs (lncRNAs) play critical regulatory roles in the tumorigenesis of GC. This study aimed to investigate the regulatory effect and mechanism of lncRNA-HCG18 on GC.

Methods: The expression of lncRNA-HCG18 was detected in GC tissues and cell lines by qRT-PCR.

Comparative efficacy of ancillary drugs in sevoflurane-related emergence agitation after paediatric adenotonsillectomy: A Bayesian network meta-analysis.

What Is Known And Objective: The comparative efficacy of ancillary drugs on sevoflurane-related emergence agitation (EA) in paediatric anaesthesia for adenotonsillectomy remains unclear. The purpose of this Bayesian network meta-analysis was to investigate the efficacy of ancillary drugs on sevoflurane-related EA in paediatric anaesthesia for adenotonsillectomy.

Methods: MEDLINE, Embase, the Cochrane Library and Web of Science databases were electronically searched to identify randomized controlled trials (RCTs) of different ancillary drugs used in adenotonsillectomy from inception to April 2019.

LncRNA DANCR affected cell growth, EMT and angiogenesis by sponging miR-345-5p through modulating Twist1 in cholangiocarcinoma.

Objective: LncRNA DANCR has been reported to play an important role in various cancers. Therefore, this study aimed at exploring the function and regulatory mechanism of DANCR in Cholangiocarcinoma (CCA).

Patients And Methods: qRT-PCR was used to measure the expression of DANCR, miR-345-5p in tissues and cells.

Long-chain non-coding RNA GACAT1 promotes development and progression of breast cancer by targeting microRNA-875-3p.

Role of long-chain non-coding ribonucleic acid (lncRNA) GACAT1 in the development of breast cancer and its possible mechanism were investigated. The levels of GACAT1, microRNA-875-3p and Stonin2 (STON2) in breast cancer tissues and adjacent normal tissues were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The level of GACAT1 in breast cancer cell lines was further explored.

Synthesis of SiO@CuSe nanospheres for efficient near-infrared radiation mediated treatment and care of gastric cancer patients.

Rational design and fabrication of suitable optical absorbing agents (OAAs) are of great significance for state-of-the-art photothermal cancer therapy. Copper selenides have recently emerged as a type of promising photothermal operators thanks to their engineered effortlessness, high carrier concentration, and superior photothermal transformation. Nevertheless, the further in vivo applications are hampered owing to their poor biocompatibility and hydrophobicity.

Combined use of circulating tumor cells and salivary mRNA to detect non-small-cell lung cancer.

Liquid biopsy is an emerging technique for noninvasive detection of various cancers. Majority of liquid biopsy tests still, however, use solitary type of biomarkers with unsatisfactory sensitivity and specificity. To this end, a combined approach of circulating tumor cells (CTCs) and salivary mRNA biomarkers was evaluated for discriminating non-small-cell lung cancer (NSCLC) from healthy controls.

miR-22 protect PC12 from ischemia/reperfusion-induced injury by targeting p53 upregulated modulator of apoptosis (PUMA).

MicroRNAs have been implicated as critical regulatory molecules in many cerebrovascular diseases. Recent studies demonstrated miR-22 might provide a potential neuroprotective effect. However, the neuroprotective effect of miR-22 in ischemia/reperfusion (I/R) injury has not been thoroughly elucidated.

Long non-coding RNA TTN-AS1 promotes breast cancer cell migration and invasion via sponging miR-140-5p.

Breast cancer (BC) is one of the most common fatal cancers. Recent studies have identified the vital role of long non-coding RNAs (lncRNAs) in the development and progression of BC. In this investigation, lncRNA TTN-AS1 was studied to identify its function in the metastasis of BC.

Dectin-1/Syk signaling triggers neuroinflammation after ischemic stroke in mice.

Background: Dendritic cell-associated C-type lectin-1 (Dectin-1) receptor has been reported to be involved in neuroinflammation in Alzheimer's disease and traumatic brain injury. The present study was designed to investigate the role of Dectin-1 and its downstream target spleen tyrosine kinase (Syk) in early brain injury after ischemic stroke using a focal cortex ischemic stroke model.

Methods: Adult male C57BL/6 J mice were subjected to a cerebral focal ischemia model of ischemic stroke.

circ-FNTA accelerates proliferation and invasion of bladder cancer.

Role of circ-FNTA in the progression of bladder cancer (BCa) and its underlying mechanism were investigated. circ-FNTA level in BCa tissues and cell lines was detected. The prognostic potential of circ-FNTA was assessed by Kaplan-Meier methods and the proliferative and invasive abilities of BCa influenced by circ-FNTA were explored.

Dietary fat intake and risk of bladder cancer: Evidence from a meta-analysis of observational studies.

The association between dietary fat intake and bladder cancer had been inconsistent in the previous epidemiological studies. The aim of this study was to investigate the difference between fat intake and bladder cancer risk. Databases of PubMed, Embase, and Web of Science were systematically searched for suitable studies from inception to June 2018.

miR-16-5p modulates the radiosensitivity of cervical cancer cells via regulating coactivator-associated arginine methyltransferase 1.

This study was to investigate the expression of coactivator-associated arginine methyltransferase 1 (CARM1) and miR-16-5p in cervical cancer (CC), and explore their roles in radioresistance. Western blot and immunohistochemistry were used to detect the expression of CARM1 in tissues and cells. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of miR-16-5p.

Rare Germline Variants Identified from Blue Rubber Bleb Nevus Syndrome Might Impact mTOR Signaling.

Background And Objective: Blue rubber bleb nevus syndrome (BRBN) or Bean syndrome is a rare Venous Malformation (VM)-associated disorder, which mostly affects the skin and gastrointestinal tract in early childhood. Somatic mutations in TEK have been identified from BRBN patients; however, the etiology of TEK mutation-negative patients of BRBN need further investigation.

Methods: Two unrelated sporadic BRBNs and one sporadic VM were firstly screened for any rare nonsilent mutation in TEK by Sanger sequencing and subsequently applied to whole-exome sequencing to identify underlying disease causative variants.