Polyprotein Processing as a Determinant for in Vitro Activity of Semliki Forest Virus Replicase.

Semliki Forest virus (SFV) is an arthropod-borne alphavirus that induces membrane invaginations (spherules) in host cells. These harbor the viral replication complexes (RC) that synthesize viral RNA. Alphaviruses have four replicase or nonstructural proteins (nsPs), nsP1-4, expressed as polyprotein P1234.

Older people coping with low mood: a qualitative study.

Background: To gain new insight into support for older people with low mood, the perceptions, strategies, and needs of older people with depressive symptoms were explored.

Methods: Two in-depth interviews were held with 38 participants (aged ≥77 years) who screened positive for depressive symptoms in general practice. To investigate the influence of the presence of complex health problems, 19 persons with and 19 without complex problems were included.

Glucocorticoid excess induces long-lasting changes in body composition in male C57Bl/6J mice only with high-fat diet.

Glucocorticoid (GC) overexposure period as observed in Cushing's syndrome (CS) is associated with the metabolic syndrome and cardiovascular disease, which persist after long-term correction of GC excess. We performed a mouse study to identify factors that modulate metabolic recovery from a GC overexposure period. Male C57Bl/6J mice, fed a low-fat diet (LFD) or a high-fat diet (HFD), received corticosterone (CORT) (50 μg/mL) or vehicle in the drinking water for 4 weeks, followed by an 8-week washout period.

Hematopoietic stem and progenitor cells are differentially mobilized depending on the duration of Flt3-ligand administration.

Background: Flt3-ligand is a cytokine that induces relatively slow mobilization of hematopoietic cells in animals and humans in vivo. This provides a time-frame to study hematopoietic stem and progenitor cell migration kinetics in detail.

Design And Methods: Mice were injected with Flt3-ligand (10 microg/day, intraperitoneally) for 3, 5, 7 and 10 days.

Buprenorphine induces ceiling in respiratory depression but not in analgesia.

Background: We measured the effect of two weight adjusted i.v. doses (0.