7 results match your criteria: "Leibniz Institute for Natural Product Research and Infection Biology eV[Affiliation]"
Multiomics Analyses of HNF4α Protein Domain Function during Human Pluripotent Stem Cell Differentiation.
iScience
June 2019
Medical Research Council Centre for Regenerative Medicine, University of Edinburgh, 5 Little France Drive, Edinburgh, Scotland EH16 4UU, UK. Electronic address:
During mammalian development, liver differentiation is driven by signals that converge on multiple transcription factor networks. The hepatocyte nuclear factor signaling network is known to be essential for hepatocyte specification and maintenance. In this study, we have generated deletion and point mutants of hepatocyte nuclear factor-4alpha (HNF4α) to precisely evaluate the function of protein domains during hepatocyte specification from human pluripotent stem cells.
View Article and Find Full Text PDFAssessment of stem cell differentiation based on genome-wide expression profiles.
Philos Trans R Soc Lond B Biol Sci
July 2018
IfADo-Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany.
In recent years, protocols have been established to differentiate stem and precursor cells into more mature cell types. However, progress in this field has been hampered by difficulties to assess the differentiation status of stem cell-derived cells in an unbiased manner. Here, we present an analysis pipeline based on published data and methods to quantify the degree of differentiation and to identify transcriptional control factors explaining differences from the intended target cells or tissues.
View Article and Find Full Text PDFGene network activity in cultivated primary hepatocytes is highly similar to diseased mammalian liver tissue.
Arch Toxicol
October 2016
IfADo-Leibniz Research Centre for Working Environment and Human Factors, Technical University of Dortmund, Ardeystrasse 67, 44139, Dortmund, Germany.
Article Synopsis
- Isolation and cultivation of primary hepatocytes lead to significant gene expression changes that mirror those seen in liver diseases.
- A comparative study of cultivated human and mouse hepatocytes revealed similarities in expression alterations between laboratory conditions and various liver conditions, such as NAFLD, cirrhosis, and HCC.
- The research identified key gene regulatory networks and suggested interventions that could minimize cultivation-induced changes, highlighting the potential of cultivated hepatocytes as models for studying liver diseases.
Corrigendum to "Gene networks and transcription factor motifs defining the differentiation of human stem cells into hepatocyte-like cells" [J Hepatol 2015;63:934-942].
J Hepatol
February 2016
IfADo-Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany. Electronic address:
To gain a broader understanding of the importance of a surface-associated lifestyle and morphogenic capability, we have assembled and annotated the genome sequences of Pseudoalteromonas strains P1-7a, P1-9, P1-13-1a, P1-16-1b, P1-25, and P1-26, isolated from Hydractinia echinata. These genomes will allow detailed studies on bacterial factors mediating interkingdom communication.
View Article and Find Full Text PDFRecombinant Laminins Drive the Differentiation and Self-Organization of hESC-Derived Hepatocytes.
Stem Cell Reports
December 2015
MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh EH16 4UU, UK. Electronic address:
Stem cell-derived somatic cells represent an unlimited resource for basic and translational science. Although promising, there are significant hurdles that must be overcome. Our focus is on the generation of the major cell type of the human liver, the hepatocyte.
View Article and Find Full Text PDFGene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cells.
J Hepatol
October 2015
IfADo-Leibniz Research Centre for Working Environment and Human Factors at the Technical University Dortmund, Dortmund, Germany. Electronic address:
Background & Aims: The differentiation of stem cells to hepatocyte-like cells (HLC) offers the perspective of unlimited supply of human hepatocytes. However, the degree of differentiation of HLC remains controversial. To obtain an unbiased characterization, we performed a transcriptomic study with HLC derived from human embryonic and induced stem cells (ESC, hiPSC) from three different laboratories.
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