Seven pregnancies and deliveries from non-mosaic Klinefelter syndrome patients using fresh and frozen testicular sperm.

Purpose: The aim of this study was to investigate the feasibility of using frozen-thawed testicular sperm as well as the timing of testicular sperm extraction (TESE) in patients with non-mosaic Klinefelter syndrome.

Methods: Intracytoplasmic sperm injection (ICSI) was performed in six of 17 (35%) patients whose sperm was recovered by TESE. Multiple biopsies of both testes were performed on the day of oocyte retrieval in all but one of the six patients.

Successful pregnancy and delivery after transfer of a single blastocyst derived from a vitrified mature human oocyte.

Objective: To describe the first case of delivery after the transfer of a single blastocyst derived from a vitrified mature human oocyte.

Design: Case report.

Setting: Private assisted reproduction clinic.

Freezing of human immature oocytes using cryoloops with Taxol in the vitrification solution.

Purpose: In human frozen immature oocytes, there has been little successful delivery. We examined the feasibility of vitrification solution including Taxol, cytoskeltal stabilizer.

Methods: We set four experimental groups that immature oocytes has cumulus cells or not, or including Taxol or not in the vitrification solution.

Physical and mental development of children after fertilization and embryo transfer.

To evaluate the physical and mental development of children after fertilization (IVF) and frozen embryo transfer (FET). Between July 1995 and November 2003, 506 patients delivered 658 babies after IVF and ET treatment at our clinic (intracytoplasmic sperm injection (ICSI), 418; conventional IVF, (C-IVF) 125; FET, 115). A survey of the physical and mental developmental of the children was conducted by mailing questionnaires to parents.

Successful pregnancy outcome using sperm from severely diseased men with testicular cancer and collagen disease: Three case reports.

To confirm the feasibility of using fresh or frozen/thawed spermatozoa from cancer and collagen diseased patients for intracytoplasmic sperm injection (ICSI). Two participants were diagnosed with testicular carcinoma and one patient was diagnosed with collagen disease. Of the two carcinoma patients, one patient provided a fresh testicular biopsy sample and one patient ejaculated fresh sperm after surgical operation.

Two-step consecutive transfer of early embryos and blastocysts.

To evaluate and confirm the merit of two consecutive transfer attempts of early embryos and blastocysts. A total of 685 patients underwent routine fertilization (IVF) cycles. The study population consisted of three groups: (i) Group 1, early stage embryos were transferred (460 patients; 567 cycles); (ii) group 2, blastocysts were transferred (88 patients; 105 cycles); and (iii) group 3, a two-step (consecutive) transfer was performed on 137 women (141 cycles).

Efficiency of using frozen-thawed testicular sperm for multiple intracytoplasmic sperm injections.

Purpose: To compare fertilization and pregnancy rates of fresh and frozen-thawed testicular sperm injections (TESE-ICSI).

Methods: Sperm collected from the testes of 28 azoospermic patients by an open testicular biopsy technique was used for initial ICSI or cryopreserved.

Results: Fresh-sperm ICSI treatment (28 cycles) resulted in a 58.

Pregnancy achieved following ICSI from a man with Klinefelter's syndrome and spinal cord injury.

Klinefelter's syndrome and spinal cord injury are major causes of male infertility. Intracytoplasmic sperm injection (ICSI) is a relatively new method of assisted reproduction. A testicular biopsy was obtained from a patient with the double complications of non-mosaic 47,XXY Klinefelter's syndrome and spinal cord damage, and motile spermatozoa were collected.