: an important genus of canker pathogens.

species have commonly been reported as important plant pathogenic fungi with wide host ranges and geographic distributions. With the increase in the number of cryptic species being described, a comprehensive global taxonomic revision of the genus is required. The present study includes 399 isolates from 32 countries.

Larval competition between the invasive Aedes aegypti (Diptera: Culicidae) and the Caribbean endemic Aedes mediovittatus (Diptera: Culicidae) from Puerto Rico, USA.

Competition between mosquito species during the larval phase is a well-established mechanism structuring container mosquito communities, with invasive species often outperforming natives. We assessed the competitive outcome between 2 species that occur on the island of Puerto Rico, the historic invasive Aedes aegypti (L.) and the endemic Aedes mediovittatus (Coquillett) (Diptera: Culicidae).

Diaci v3.0: chromosome-level assembly, de novo transcriptome, and manual annotation of Diaphorina citri, insect vector of Huanglongbing.

Background: Diaphorina citri is an insect vector of "Candidatus Liberibacter asiaticus" (CLas), the gram-negative bacterial pathogen associated with citrus greening disease. Control measures rely on pesticides with negative impacts on the environment, natural ecosystems, and human and animal health. In contrast, gene-targeting methods have the potential to specifically target the vector species and/or reduce pathogen transmission.

The status of insecticide resistance of Anopheles coluzzii on the islands of São Tomé and Príncipe, after 20 years of malaria vector control.

Background: Insecticide-based malaria vector control has been implemented on the islands of São Tomé and Príncipe (STP) for more than 20 years. During this period malaria incidence was significantly reduced to pre-elimination levels. While cases remained low since 2015, these have significantly increased in the last year, challenging the commitment of the country to achieve malaria elimination by 2025.

Occurrence of Snake River alfalfa virus in alfalfa () in Oregon and in Northern California.

Alfalfa (Medicago sativa L.) is a commonly grown forage crop in Oregon and California harvested on 350,000 and 480,000 acres, respectively, in 2023 (USDA-NASS 2023). Forage alfalfa is grown as a perennial crop for about four years in the same field and each season, the crop is cut 3-4 times for hay production.

First detection of Bemisia tabaci (Hemiptera: Aleyrodidae) MED in Oklahoma and development of a high-resolution melting assay for MEAM1 and MED discrimination.

The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is a polyphagous pest recognized as composed of several cryptic or sibling species. The Middle East-Asia Minor 1 (MEAM1) and the Mediterranean (MED) putative species are invasive and destructive worldwide. The MEAM1 is established throughout the United States, while MED is documented in 27 states.

Water sources selected for immature development of some African rainforest dwelling mosquitoes under different landscapes in Cameroon.

Little is known about the behaviors of African equatorial rain forest mosquito species and their potential role as sylvatic and bridge-vectors of various pathogens of animal and public health. In 2016 and 2017, the diversity and sources of water supporting immature development of mosquitoes in Talangaye Rainforest (South West Cameroon) before, during and after deforestation were investigated. Mosquito eggs, larvae and pupae were collected from 12 natural, seminatural, and artificial water sources and reared to adults.

Effect of various physical and chemical stress conditions on the infectivity and survival of Heterorhabditis indica and Steinernema feltiae: Relationship with lipid oxidative stress.

Entomopathogenic nematodes (EPNs) of the genera Heterorhabditis and Steinernema represent an alternative for the biological control of insects. The limited half-life of EPNs is still one of the most concerning issues in their commercialization. Lipid peroxidation (LPO) caused by reactive oxygen species (ROS) may be one of the most important causes of loss of infectivity and survival of EPNs when exposed to various physicochemical stress conditions (temperature, pH, hypoxia and osmotic pressure).

Sustained antiviral insulin signaling during West Nile virus infection results in viral mutations.

Arthropod-borne viruses or arboviruses, including West Nile virus (WNV), dengue virus (DENV), and Zika virus (ZIKV) pose significant threats to public health. It is imperative to develop novel methods to control these mosquito-borne viral infections. We previously showed that insulin/insulin-like growth factor-1 signaling (IIS)-dependent activation of ERK and JAK-STAT signaling has significant antiviral activity in insects and human cells.

AEDEOMYIA SQUAMIPENNIS: A NEW GENUS AND SPECIES RECORD FOR COLLIER COUNTY, FLORIDA, USA.

On November 7, 2023, 3 female specimens of Aedeomyia squamipennis were captured in Collier County, Florida, for the first time during routine adult mosquito surveillance in Collier Seminole State Park. This species was first found in the USA in Miami-Dade County in 2016 and has since expanded into multiple counties throughout South Florida. The presence of Ad.

In Vitro Assessment of Sensitivity to Difenoconazole.

causes blue mold, a major post-harvest disease affecting apples. This disease is commonly managed using fungicides, including Difenoconazole (Dif), a demethylation inhibitor (DMI) approved for its control. This investigation aims to evaluate the baseline sensitivity of 100 isolates to Difenoconazole.

Evaluation of tree-injected oxytetracycline and antisense oligonucleotides targeting Candidatus Liberibacter asiaticus in citrus.

Background: Huanglongbing (HLB) is a systemic disease of citrus caused by the bacterial pathogen Candidatus Liberibacter asiaticus (CLas) that limits citrus production worldwide. CLas is an obligate bacterial pathogen that multiplies in citrus trees and in the insect vector, the Asian citrus psyllid (ACP), Diaphorina citri Kuwayama. There is no cure for HLB currently and broad-spectrum antibiotics represent one possible therapeutic against disease symptoms.

Enhanced nanobody-driven bioluminescent immunoassay for rapid parathion detection using engineered split-nanoluciferase.

In this work, with parathion, a typical forbidden organophosphate pesticide as target drug, an enhanced nanobody-driven bioluminescent immunoassay based on the engineered split-nanoluciferase (NanoLuc) was proposed. Concretely, through labeling 11S and β10, two split-NanoLuc units onto the anti-parathion nanobody (Nb) VHH9 and the artificial antigen H1 coupled with carrier protein ovalbumin (H1-OVA) respectively, an NanoLuc Binary Technology (NanoBiT) system was firstly developed in the form of homogeneous immunoassay, in which the luminescence signal was produced by the reassembled NanoLuc after the combination of the 11S-fused VHH9 and β10-labeled H1-OVA. Subsequently, in order to enhance the signal-to-noise (S/N) ratio, a novel strategy of splitting 11S into two smaller subunits Δ11S and β9 was adopted so then an NanoLuc Ternary Technology (NanoTeT) system based on tri-part components of β9-fused VHH9, β10-labeled H1-OVA and Δ11S was successfully established.

n. sp. (Nematoda: Heteroderinae), a new species of cyst-forming nematode from Southwest China, with a key to the Genus .

The cyst nematodes, subfamily Heteroderinae, are plant pathogens of worldwide economic significance. A new cyst nematode of the genus within the Heteroderinae, n. sp.

Deciphering the vectors: Unveiling the local dispersal of Litylenchus crenatae ssp. mccannii in the American beech (Fagus grandifolia) forest ecosystem.

Beech leaf disease (BLD), caused by the Litylenchus crenatae ssp. mccannii (Lcm) nematode, is an emerging threat to beech trees. This disease is characterized by distinct leaf symptoms, including leaf interveinal banding and thickened leaf texture, which leads to eventual tree mortality.

Uncovering the fungus responsible for stem and root rot of false indigo: pathogen identification, new disease description, and genome analyses.

Calonectria spp. can cause destructive diseases on forestry crops, legumes like soybean and peanut, and ornamentals. Species of Calonectria affecting ornamental plants are not well characterized or understood, though they have been widely documented as an issue in the ornamental industry.

Fabrication of a high-sensitivity electrochemical immunosensor by the oriented immobilization of engineered nanobody on nanofibrous membrane.

A new type of label-free electrochemical immunosensor for the high-sensitivity determination of parathion was developed based on the oriented immobilization of nanobody (VHH9) on a gold nanoparticle-loaded polyvinyl alcohol/citric acid nanofiber membrane-modified electrode. The morphology characterization and assembly process of the modified materials were investigated using scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Under the optimum conditions, the label-free electrochemical immunosensor for parathion exhibited a linear range of 0.

Cover crop rotation suppresses root-knot nematode infection by shaping soil microbiota.

Cover crop integration into grain crop rotations is a promising strategy for mitigating nematode-induced diseases in agriculture. However, the precise mechanisms underlying this phenomenon remain elusive. Here, we first assessed the impact of five commonly used cover crops on the suppression of rice root-knot nematodes (RKNs).

Protorhabditis nematodes and pathogen-antagonistic bacteria interactively promote plant health.

Background: Fertilization practices control bacterial wilt-causing Ralstonia solanacearum by shaping the soil microbiome. This microbiome is the start of food webs, in which nematodes act as major microbiome predators. However, the multitrophic links between nematodes and the performance of R.

Evaluation of host status of garlic varieties for a plant-parasitic nematode, , by using in vitro inoculation.

is a plant-parasitic nematode that severely damages garlic ( L.) in Japan. is detected in roots, bulbs, and outer bulb skins of garlic at harvest; however, the resistance of garlic to infection is not well understood.

Response of hibiscus mealybug (Hemiptera: Pseudococcidae) to citrus volatiles induced by mechanical injury.

Hibiscus mealybug, Nipaecoccus viridis (Newstead) (Hemiptera: Pseudococcidae), is a recent invasive pest of citrus and many other crops in Florida. Nipaecoccus viridis attacks all above ground parts of citrus trees and heavy infestation can cause leaf drop and premature abortion of developing fruits. We quantified greater captures of N.