29 results match your criteria: "Laboratório de Pesquisa Em Infecção Hospitalar (LAPIH)[Affiliation]"

Comparative analysis of the antimicrobial resistance and virulence traits in ESBL-producing-Klebsiella pneumoniae ST307 strains colonizing the gastrointestinal tract and causing a fatal bloodstream infection in a leukemia patient.

Infect Genet Evol

July 2024

Laboratório de Investigação em Microbiologia Médica (LIMM), Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil; Departamento de Hidrobiologia, Universidade Federal de São Carlos, São Carlos, Brazil. Electronic address:

Klebsiella pneumoniae is an opportunistic pathogen that can colonize the gastrointestinal tract (GIT) of humans. The mechanisms underlying the successful translocation of this pathogen to cause extra-intestinal infections remain unknown, although virulence and antimicrobial resistance traits likely play significant roles in the establishment of infections. We investigated K.

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  • Carbapenemase production is a significant global health concern, and analyzing antimicrobial resistance (AMR) data is essential for effective public health policies, as showcased in this study focused on Brazil's AMR Surveillance Network.
  • The study evaluated carbapenemase detection rates in Brazilian hospitals from 2015 to 2022, finding trends such as a notable increase in blaNDM detection and a decrease in blaKPC for Enterobacterales, particularly after the onset of the COVID-19 pandemic.
  • Overall, the findings highlight the resilience of the AMR Surveillance Network and indicate a concerning shift in carbapenemase profiles, with blaNDM becoming increasingly prevalent.
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  • Pseudomonas aeruginosa and Acinetobacter calcoaceticus-baumanii complex are dangerous pathogens in hospitals, with the ability to develop resistance to antibiotics and cause serious outbreaks.
  • This research focused on identifying Metallo-β-lactamases (MBLs) in Paraguay's hospitals and examined the coexistence of different carbapenemases.
  • The study found 70 MBL-producing strains primarily from Pseudomonas and Acinetobacter species, showing clonal spread in some cases and evidence of transmission between different bacterial strains.
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  • Carbapenems are crucial last-resort antibiotics for treating infections from multidrug-resistant Gram-negative bacteria, but resistance through mechanisms like OprD loss and carbapenemases is a growing global concern.
  • A study in Brazil tested the effectiveness of three phenotypic tests—Carba NP, Blue Carba, and mCIM/eCIM—to detect carbapenemase-producing Pseudomonas species, using 127 clinical isolates.
  • Results showed that Carba NP had the highest sensitivity and successfully differentiated between carbapenemases types, while Blue Carba struggled with class B detection, highlighting the need for improved and more reliable resistance detection methods.
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  • - The study assessed 502 Enterobacteriales isolates in Brazil for resistance to polymyxins, specifically colistin, revealing a 29.5% resistance rate and confirming all isolates as multidrug-resistant (MDR).
  • - Genetic analysis indicated that 77% of isolates harbored carbapenemase genes, with various molecular typing methods identifying significant diversity, including 51 pulsotypes and 21 sequence types (STs), notably ST437, ST258, and ST11.
  • - The research identified key chromosomal mutations and insertion sequences linked to increased resistance, highlighting the emergence of high-risk clones such as CC11 in Brazil, raising concerns about global implications for antibiotic resistance.
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  • High rates of carbapenem resistance in Brazilian Pseudomonas aeruginosa are linked to a specific clone (ST277) that produces a type of carbapenemase called SPM-1.
  • Researchers used whole genome sequencing to analyze the genetic make-up of a plasmid containing the bla gene in these resistant isolates and found a new bla-plasmid.
  • The study highlights the urgent need for public health measures to track the spread of this plasmid due to its connection with a high-risk bacterial clone.
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  • - Emergence of colistin-resistant bacteria with mobile resistance genes is a growing threat to both animals and humans involved in food production.
  • - The article focuses on identifying a specific plasmid that carries colistin resistance genes in poultry, highlighting its role as a new reservoir for these genes.
  • - Three closely related bacterial strains were isolated from cloacal swabs, and their genomes included a significant plasmid that potentially facilitates the spread of colistin resistance.
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Genomic information on Stenotrophomonas maltophilia ST264 isolated from a cystic fibrosis pediatric patient in Brazil.

Braz J Microbiol

September 2020

Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Avenida Boulevard 28 de Setembro, 87, fundos, 3° andar, Vila Isabel, Rio de Janeiro, RJ, 20551-030, Brazil.

Stenotrophomonas maltophilia is one of the Gram-negative bacilli most frequently found in the airways of cystic fibrosis patients. This opportunistic pathogen is intrinsically multidrug-resistant, and therefore, its treatment presents a challenge. The genetic characterization of S.

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  • KPC-producing bacteria have emerged as a significant public health concern, particularly as previous colonization is a key risk factor for developing infections.
  • A study analyzed 102 KPC- isolates from human rectal swabs across Brazil to understand their genetic and antimicrobial resistance profiles between 2009-2013.
  • Results showed these isolates were largely resistant to various antibiotics but susceptible to a few, with many belonging to a specific genetic lineage (clonal complex 258), indicating a strong link between colonization and infection in patients.
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Draft Genome Sequence of a Wohlfahrtiimonas chitiniclastica Strain Isolated from Frozen Chicken in Rio De Janeiro, Brazil.

Microbiol Resour Announc

December 2019

Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas (FCM), Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

Here, we report the draft genome sequence of strain 20, isolated from a chicken carcass originated from indoor broiler farming and identified using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry followed by sequencing of the 16S rRNA gene.

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In Brazil, A. baumannii has been described as nosocomial pathogens causing hospital-acquired infections. Current WGS technologies have been useful in identifying of genetic features between Acinetobacter isolates.

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New Delhi metallo-β-lactamase (NDM)-producing bacteria have been identified at a worrying rate in Brazil since 2013. Owing to the need to understand the extent of their spread, this study reports the dissemination of bla in different species of Gram-negative bacilli in different regions and states of Brazil. A total of 81 isolates from nine states were studied, including 11 species.

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  • This study focuses on a specific isolate of Klebsiella pneumoniae that is extensively drug-resistant (XDR) and produces the KPC-2 enzyme.
  • Whole-genome sequencing was used to analyze its genetic characteristics and understand its clinical background.
  • The isolate is part of clonal complex 258 and contains various genes and mutations that contribute to its resistance to multiple antibiotics.
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In this study, we report the isolation of OXA-72-producing Acinetobacter pittii in Brazil. A carbapenem-resistant A. pittii strain was recovered from a hospitalized female patient from Espírito Santo, Southeastern Brazil.

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The distinction between healthcare-associated MRSA (HA-MRSA) and community-associated MRSA (CA-MRSA) infections has become increasingly blurred. We assessed the molecular characterization and antimicrobial resistance profile for MRSA isolates from blood. Most of all (81.

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We aimed to describe an early detection of OXA-370-producing Klebsiella pneumoniae in Brazil. The isolate CCBH10079 belonged to ST17 and the bla was located in a plasmid of ≅57kb.

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  • The study focused on analyzing polymyxin B (PMB) resistance in 126 Klebsiella pneumoniae isolates collected from rectal swabs in Brazil.
  • Out of these, 10 isolates showed PMB resistance, primarily due to interruptions in the mgrB gene, either through insertion sequences or mutations.
  • Most resistant isolates also carried the bla gene and were mainly part of clonal complex 258, emphasizing the need to monitor the spread of PMB-resistant bacteria in hospital settings to address the limited treatment options for multidrug-resistant infections.
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This study reveals the presence of different carbapenemase genes (blaKPC, blaNDM, blaGES, and blaOXA48-like genes) detected directly from water samples and clonal dispersion (by pulsed-field gel electrophoresis [PFGE] and multilocus sequence typing [MLST]) of KPC-2-producing Enterobacteriaceae in two important urban aquatic matrixes from Rio de Janeiro, Brazil, highlighting the role of aquatic environments as gene pools and the possibility of community spreading.

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Complete genome sequence of Haloferax gibbonsii strain ARA6, a potential producer of polyhydroxyalkanoates and halocins isolated from Araruama, Rio de Janeiro, Brasil.

J Biotechnol

October 2015

Instituto de Biologia Roberto Alcântara Gomes, Departamento de Bioquímica, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address:

Haloferax gibbonsii strain ARA6 is a haloarchaea isolated from saline saltern samples from Vermelha lake, located in Araruama region, Rio de Janeiro, Brazil. Its genome displays 66,2% G+C content and is composed by one circular chromosome of 2,945,391 bp and four circular plasmids comprising 993,063 bp. This genomic information shows H.

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Introduction: Yersinia enterocolitica is a well-known foodborne pathogen widely distributed in nature with high public health relevance, especially in Europe.

Methodology: This study aimed to analyze the pathogenic potential of Y. enterocolitica isolated strains from human, animal, food, and environmental sources and from different regions of Brazil by detecting virulence genes inv, ail, ystA, and virF through polymerase chain reaction (PCR), phenotypic tests, and antimicrobial susceptibility analysis.

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The most important resistance mechanism against β-lactam drugs is the production of carbapenemases. In this study, we report the first identification of Klebsiella pneumoniae carbapenemase (KPC)-2 and New Delhi metallo-β-lactamase (NDM)-1 in Enterobacter hormaechei subps. oharae from Brazil.

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New Delhi metallo-β-lactamase 1 (NDM-1) was first identified in Brazil in Enterobacter hormaechei and Providencia rettgeri in 2013. Here, we describe the first case of NDM-1-producing Acinetobacter baumannii sequence type 25 isolated from the urinary tract of a 71-year-old man who died of multiple complications, including A. baumannii infection.

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