Isolation, Identification, and Characterization of L-asparaginase-Producing Human Commensal Bacterial Strains: A Promising Next-Gen Probiotics.

L-asparaginase is an FDA-approved drug for treating blood cancer, but its inherent antigenicity and L-glutaminase activity are associated with hypersensitivity and organ toxicity. Extracellularly produced glutaminase-free L-asparaginase from human commensal bacteria may be a good alternative to reduce the side effects of therapeutic L-asparaginase. Here, we report the isolation and characterization of fourteen L-asparaginase-producing bacterial strains belonging to the genera Acinetobacter, Escherichia, Klebsiella, and Pseudomonas from human stool and saliva samples.

Molecular characterization of typing and subtyping of Staphylococcal cassette chromosome SCC types I to V in methicillin-resistant from clinical isolates from COVID-19 patients.

Background And Objectives: Methicillin resistance is acquired by the bacterium due to gene which codes for penicillin-binding protein (PBP2a) having low affinity for β-lactam antibiotics. gene is located on a mobile genetic element called staphylococcal cassette chromosome (SCC). SCC genomic island comprises two site-specific recombinase genes namely and [cassette chromosome recombinase] accountable for mobility.

Recent advances in research, training and teaching in anaesthesia and critical care.

Teaching and learning in anaesthesia and critical care has traditionally included seminars, journal clubs and clinical teaching in the operation theatre. The aim has always been to ignite the spark of thinking and self-directed learning in the students. The process of 'dissertation preparation' introduces basic knowledge and interest in research in the postgraduate student.

How to do a journal club, a seminar and a webinar?

The topics for postgraduate teaching-learning tools are Journal club in-house with one speaker and a moderator, Seminars- with multiple speakers and a co-ordinator, and Webinars- online seminars with one or multiple speakers choosing multiple mediums of communication. They largely affect the working mechanism of a clinician as they help us upgrade with the recent development in our fields. Making them interesting for us as well as our colleagues is necessary.