Enhancing effect of ethanol on aflatoxin b1-induced DNA-damage in glutathione-depleted rat hepatocytes.

The effect of reduced glutathione (GSH) and ethanol on aflatoxin B1 (AFB1)-induced DNA single strand breaks was studied in primary cultured hepatocytes. Buthionine sulfoximine (BSO) which decreased intracellular GSH to 13% of those of the control levels increased DNA fragmentation of AFB1-treated hepatocytes by over 17% of those without BSO. Thus, a decrease in hepatocyte GSH levels increased AFB1-induced DNA damage.

Expression of hepatocyte growth factor(hgf) and C-met gene in human gastric-cancer cell-lines.

It has become evident that the hepatocyte growth factor (HGF) could be involved in the growth of various epithelial cells. In addition, it has recently been elucidated that the c-met gene, a proto-oncogene, encodes the HGF receptor. We examined the expression of HGF and c-met gene in human gastric cancer, using seven gastric cancer cell lines.

Small-cell carcinoma of the gallbladder - a clinicopathological, immunohistochemical and flow cytometric study of 15 cases.

Small cell carcinoma (SC) of the gallbladder is an uncommon tumor, and not enough information of this tumor has been previously reported. The SCs in this series occured in nine women and six men (mean age; 64.5 years).

L-histidinol potentiates hyperthermic cell-killing in-vitro.

To clarify the effect of L-histidinol on hyperthermic killing of cells, HeLa and mouse sarcoma-180 (S-180) cells were exposed to heat in vitro in the presence of L-histidinol and the clonogenic surviving fraction of the cells was examined. After pretreating the cells with L-histidinol for 4 h, exposure of the cells to the combination of heat at 43-degrees-C and L-histidinol for various times (30 min to 4 h) reduced the surviving fraction more prominently than heat alone. Optimal concentrations to confer effective enhancement of heat on HeLa and S-180 cells were 9 mM and 3 mM, respectively.

Prognosis for gastric-cancer invading the serosa evaluated by argyrophilic nucleolar organizer region staining.

Eighty-five patients with gastric cancer invading the serosa following curative resection were analyzed with respect to clinicopathological features and prognosis by staining the argyrophilic nucleolar organizer region (AgNOR). The AgNOR was counted in 200 cancer cells per tissue and the mean number per cell was identified as the AgNOR score. The AgNOR count varied from 1.

Gastric-carcinoma developing in the reconstructed gastric tube following esophagectomy - analysis of 58 cases.

Fifty-eight Japanese patients with secondary adenocarcinoma, which developed in the reconstructed gastric tube after esophagectomy for carcinoma of the esophagus, were reviewed from 20 case reports published over a period of twenty years. Following esophagectomy the gastric tube was replaced through the antesternal route in 32 patients (55.2%), through the retrosternal route in 19 (32.

Growth-inhibition and change in morphology and motility of sclc cell-lines by hepatocyte growth-factor scatter factor.

Expression of the c-met proto-oncogene is common iii small cell lung cancer (SCLC) cell lines. The product of this proto-oncogene is the receptor for hepatocyte growth factor (HGF), also known as scatter factor (SF) and hepatopoietin A. We examined the effect of HGF/SF on 8 SCLC cell lines of which six expressed c-Met and 2 control cell lines did not.

Cytotoxic and cytostatic effects of polyphenols against rat 3y1 fibroblasts transformed by e1a gene of human adenovirus-type-12.

Toxicity of polyphenols against rat 3Y1 fibroblasts and the cells transformed by human adenovirus (Ad12-3Y1), its EIA gene (EIA-3Y1), or simian virus 40 (SV-3Y1) was examined. Among the diphenol compounds examined, pyrocatechol (o-diphenol) and hydroquinone (p-diphenol) showed selective toxicity against Ad12-3Y1 and EIA-3Y1 cells, while resorcinol (m-diphenol) showed a much weaker non-specific toxicity against these cells. Another o-diphenol (dopamine) and triphenols (gallic acid and pyrogallol) were less toxic but showed selective toxicity.

Liver adenosquamous carcinoma invading the esophagogastric junction - a case-report and a review of the literature.

A case of adenosquamous carcinoma of the liver involving the esophago-gastric junction is reported. The preoperative diagnosis of a submucosal tumor of the cardia of the stomach was made following a barium meal study, gastrofiberscopy, ultrasonography, and computed tomography. At surgery, a tumor was found measuring 6x5 cm in diameter and involving the left lobe of the liver, lower esophagus and cardia of the stomach, and the origin of the tumor was unclear.

Simple and efficient chemosensitivity assay for human primary tumor-cells using contact-sensitive microtiter plates.

A simple and efficient chemosensitivity assay for human primary tumors has been developed using 96-well microtiter plates covered totally with lethally irradiated 3T3 monolayers termed cell mats, on which the proliferation of normal human fibroblasts is preferentially inhibited. Two days after the inoculation of tumor cells into the microtiter cell mat plates, the cells were treated with various anticancer drugs, and the cell numbers were assessed by radioactivity using a 96-well automatic scintillation counter after subsequent radiolabeling with H-3-deoxyuridine for 24h. In this manner, complete dose response curves of many anticancer drugs were available from one plate within 5 days.

[Surgical treatment of endometrial cancer].

The main treatment of endometrial cancer is surgery. Total abdominal hysterectomy (TAH) and bilateral salpingo-oophorectomy (BSO) is the principal surgery for stage I disease. Recently, pelvic lymph node dissection and para-aortic node biopsy is also being performed.

[Case-control studies of hepatocellular carcinoma and liver cirrhosis in relation to life style and other risk factors].

Case-control studies were initiated to investigate possible risk factors (life style, etc.) for hepatocellular carcinoma (HCC) and liver cirrhosis (LC). One hundred and fifty-four patients with HCC and 97 patients with LC who had no evidence of HCC were compared with frequency-matched 298 control subjects.

[Prospective prediction of radiotherapy induced normal tissue injuries].

Variation of the normal tissue response to radiotherapy in animals of a given age, sex and species, and predictive analysis of the post radiotherapy normal tissue injuries for each case were reviewed. The degree of response of mouse hind legs to 25 Gy in a single dose varies from less than 20 Gy equivalent to more than 30 Gy equivalent injuries, implying that there still remains an unknown dose modifying factor whose modification factor is at large. It has been proposed to predict prospectively the post irradiation response of several normal tissues including the lung.

[Interstitial radiotherapy of carcinoma of the tongue-comparison between Jacobson-Yamamoto grading and prognosis].

One hundred and one patients of less than seventy years old with T1, T2 and N0 carcinoma of the tongue were graded according to the Jacobson-Yamamoto's grading (1-4 D). All patients were also classified into two groups such as less than fifty years old group and fifty to seventy years old group. Patients with grade 3, 4 C and 4 D carcinoma had more metastases to the cervical lymph nodes than grade 1, 2.

[An evaluation of scalene lymph node metastasis in patients with gynecologic malignancies].

From 1981, through 1985, 90 patients with primary or recurrent gynecologic malignancies underwent a scalene lymph nodes (SLN) biopsy. Pelvic lymph nodes (PN) and paraaortic lymph nodes (PAN) also were examined for metastasis with CT, lymphography, and palpation or were biopsied after a laparotomy. Twenty-three of these 90 patients (25.

[Pulmonary tuberculosis in patients with oral cancer].

During an 11 year period, from 1976 to 1986, 33 (10.1%) out of 328 patients with oral cancer treated at our clinic had either a scarred pulmonary tuberculosis (TB) or active TB. Four of these 33 patients developed active TB.

[A follicular, medium-sized cell lymphoma showing minimal progress in the last 13 years--case report].

A case history of a 39-year-old woman with a follicular, medium-size cell type lymphoma (Nodular poorly differentiated lymphocytic lymphoma) is reported. In spite of receiving no treatment, her lymphoma had changed little for 13 years. This case is regarded as a good example for considering the treatment of low grade malignant lymphomas.

[Evaluation of paraaortic lymph node metastasis in patients with primary epithelial carcinoma of the ovary].

From 1979-1986, 110 patients with an ovarian cancer have been treated at Kyushu University Hospital. Sixty-eight of these patients with untreated primary adenocarcinoma of the ovary underwent a paraaortic lymph node (PAN) biopsy at the time of their initial surgery. Twenty-two of these 68 patients were found (32.

[Primary ileal adenocarcinoma, showing squamous differentiation and ossification].

A 55-year-old man underwent an ileocecal resection because of an intestinal obstruction. The resected specimen contained a sessile tumor near the ileal end. This tumor was 9 x 7 cm in size, with a nodular surface of brown tint.

[Analysis of non-Hodgkin's lymphomas of 210 patients with nodular and favorable histologies--report of the Japanese Lymphoma Radiation Therapy Study Group].

Between 1972 and 1985, 210 patients with nodular poorly differentiated lymphocytic (NPDL), nodular mixed lymphocytic and histiocytic (NM), nodular histiocytic (NH), or diffuse well differentiated lymphocytic (DWDL) lymphoma according to Rappaport classification were treated among the 7 hospitals belonging to JLRTG (Japanese Lymphoma Radiation Therapy Study Group). Age greater than 50 years adversely affected overall survival. But no obvious survival differences were seen by sex and the primary site.

[Improved survival rates in Hodgkin's disease].

Results of treatment of 25 Hodgkin's disease patients at Kyushu University Hospital from 1981 to 1985 were reviewed. Total nodal irradiation or MOPP chemotherapy was used mainly. The actuarial 5-year survival rate, 87.

[Superior vena cava syndrome associated with carcinoma of the uterine cervix--report of two cases].

Superior vena cava syndrome (SVCS) is a very rare complication in patients with a gynecologic malignancy. Only 4 cases have been reported in the English literature thus far and, to our knowledge, no such case has been reported in the Japanese literature. We have encountered two cases of SVCS in patients with cervical cancer.

[Evaluation of para-aortic lymph node metastases in patients with cervical cancer].

From 1979 to 1986, 355 patients with cervical cancer underwent paraaortic lymph node (PAN) biopsy before the start of initial treatment. Nineteen of these 355 patients (5.4%) had a positive PAN, and the percentage incidence of positive PAN in each clinical stage was as follow: stage Ib, 2.

[The role of "beta-endorphin & beta-lipotropin" on the gonadotropin regulation in the mechanism of human ovulation].

The aim of this study is to elucidate the role of "beta-endorphin (beta-End) & beta-lipotropin (beta-LPH)" on the regulation of gonadotropin (Gn) secretion. We investigated the relationships between immunoreactive beta-End plus beta-LPH and Gn in peripheral plasma of normal menstruating women, 1) during periovulatory period, especially at the time of Gn surge, 2) at the estrogen induced Gn surge (positive feedback) and 3) at the time of hypoxic stress. Plasma concentrations of beta-End plus beta-LPH were measured as immunoreactive beta-End (i-beta-End) by RIA after extraction with Sep-Pak C18.