Highly sensitive two-dimensional profiling of N-linked glycans by hydrophilic interaction liquid chromatography and dual stacking capillary gel electrophoresis.

Background: N-Glycosylation is one of the most important post-translational modifications in proteins. As the N-glycan profiles in biological samples are diverse and change according to the pathological condition, various profiling methods have been developed, such as liquid chromatography (LC), capillary electrophoresis (CE), and mass spectrometry. However, conventional analytical methods have limitations in sensitivity and/or resolution, hindering the discovery of minor but specific N-glycans that are important both in the basic glycobiology research and in the medical application as biomarkers.

Fast, efficient, narrowband room-temperature phosphorescence from metal-free 1,2-diketones: rational design and the mechanism.

We report metal-free organic 1,2-diketones that exhibit fast and highly efficient room-temperature phosphorescence (RTP) with high colour purity under various conditions, including solutions. RTP quantum yields reached 38.2% in solution under Ar, 54% in a polymer matrix in air, and 50% in crystalline solids in air.

Giant anisotropic thermal expansion of copper-cyanido flat layers with flexible copper nodes.

Flat layers are usually considered as structurally rigid motifs in two-dimensional (2D) materials. In this work, we demonstrate that a flat honeycomb-layer composed of distorted tri-coordinate copper ions bridged with cyanidos in (tetraethylammonium)Cu(CN) exhibits high in-plane flexibility. This resulted in an extremely large anisotropic 2D-thermal expansion.

Identification of lipid-specific proteins with high-density lipid-immobilized beads.

In biological membranes, lipids often interact with membrane proteins (MPs), regulating the localization and activity of MPs in cells. Although elucidating lipid-MP interactions is critical to comprehend the physiological roles of lipids, a systematic and comprehensive identification of lipid-binding proteins has not been adequately established. Therefore, we report the development of lipid-immobilized beads where lipid molecules were covalently immobilized.

Bioisostere-conjugated fluorescent probes for live-cell protein imaging without non-specific organelle accumulation.

Specific labeling of proteins using membrane-permeable fluorescent probes is a powerful technique for bioimaging. Cationic fluorescent dyes with high fluorescence quantum yield, photostability, and water solubility provide highly useful scaffolds for protein-labeling probes. However, cationic probes generally show undesired accumulation in organelles, which causes a false-positive signal in localization analysis.

Metal-organic framework/NbCT MXene composites for ultrasensitive detection of dopamine.

An easy, growth approach led to the formation of several composites of metal-organic framewoks and NbCT MXenes mixed intimately at the submicron scale. The high affinity of MXene surface for dopamine, enhanced by a nanostructuration induced by MOFs, resulted in superior sensing performances. The system exhibited good linearity over the 1-100 nM range, with an excellent limit of detection of 0.

Reaction of size-selected iron-oxide cluster cations with methane: a model study of rapid methane loss in Mars' atmosphere.

We report gas-phase reactions of free iron-oxide clusters, FeO, and their Ar adducts with methane in the context of chemical processes in Mars' atmosphere. Methane activation was observed to produce FeOCH/FeOCD and FeOC, where the reactivity exhibited size and composition dependence. For example, the rate coefficients of methane activation for FeO and FeO were estimated to be 1 × 10 and 3 × 10 cm s, respectively.

Determination of the point resolution of high-resolution transmission electron microscope using the through-focus technique.

From the viewpoint of evaluating the instrumental performance of high-resolution electron microscopy (HREM), the Scherzer condition was investigated using information theory. As a result, the optimum defocus amount Δf can be expressed based on [Formula: see text] , and the formula [Formula: see text] is obtained. Furthermore, a procedure for measuring point resolution using the through-focus technique is developed, and a new method for determining the spherical aberration coefficient using the variance of Δf is introduced in the procedure.

Recent progress in application of surface X-ray scattering techniques to soft interfacial films.

X-ray reflection (XR) and surface grazing incidence X-ray diffraction GIXD) techniques have traditionally been used to evaluate the structure of soft interfacial films. In recent years, the use of synchrotron radiation and two-dimensional detectors has enabled high resolution and high speed measurements of interfacial films, which makes it possible to evaluate more detailed and complex interfacial film structures and adsorption dynamics. In this review, we will provide an overview of recent progress in structural characterization of simple oil/water interfaces, interfacial films of biologically relevant materials, oil/water interfaces for extraction of rare metal ions, and adsorption of nanoparticles.

No-wash fluorogenic labeling of proteins for reversible photoswitching in live cells.

Photoswitchable fluorescent molecules (PSFMs) are positioned as valuable tools for biomolecule localization tracking and super-resolution imaging technologies due to their unique ability to reversibly control fluorescence intensity upon light irradiation. Despite the high demand for PSFMs that are suitable for live-cell imaging, no general method has been reported that enables reversible fluorescence control on proteins of interest in living cells. Herein, we have established a platform to realize reversible fluorescence switching in living cells by adapting a protein labeling system.

Gold nanoparticle-powered screening of membrane protein-specific lipids from complex lipid mixtures.

Membrane proteins (MPs) are affected by binding of specific lipids. We previously developed a methodology for systematically analyzing MP-lipid interactions leveraging surface plasmon resonance (SPR). In this method, the gold sensor chip surface was modified with a self-assembled monolayer (SAM), which allowed for a larger amount of MP-immobilization.

Truncated derivatives of amphidinol 3 reveal the functional role of polyol chain in sterol-recognition and pore formation.

Here we examined the membrane binding and pore formation of amphidinol 3 (AM3) and its truncated synthetic derivatives. Importantly, both of the membrane affinity and pore formation activity were well correlated with the reported antifungal activity. Our data clearly demonstrated that the C1-C30 moiety of AM3 plays essential roles both in sterol recognition and stable pore formation.

Impaired biosynthesis of ergosterol confers resistance to complex sphingolipid biosynthesis inhibitor aureobasidin A in a PDR16-dependent manner.

Complex sphingolipids and sterols are coordinately involved in various cellular functions, e.g. the formation of lipid microdomains.

Efficient water-based purification of metal-organic polyhedra by centrifugal ultrafiltration.

An efficient water-based purification strategy for metal-organic polyhedra (MOPs) using commercially available centrifugal ultrafiltration membranes was developed. Having a diameter above 3 nm, MOPs were almost fully retained by the filters, while free ligands and other impurities were washed away. MOP retention also enabled efficient counter-ion exchange.

Visualization of multiple localizations of GLUT4 by fluorescent probes of PYP-tag with designed unnatural warhead.

Within a cell, multiple copies of the same protein coexist in different pathways and behave differently. Being able to individually analyze the constant actions of proteins in a cell is crucial to know the pathways through which they pass and which physiological functions they are deeply involved in. However, until now, it has been difficult to distinguish protein copies with distinct translocation properties by fluorescence labeling with different colors in living cells.

Singlet fission as a polarized spin generator for dynamic nuclear polarization.

Singlet fission (SF), converting a singlet excited state into a spin-correlated triplet-pair state, is an effective way to generate a spin quintet state in organic materials. Although its application to photovoltaics as an exciton multiplier has been extensively studied, the use of its unique spin degree of freedom has been largely unexplored. Here, we demonstrate that the spin polarization of the quintet multiexcitons generated by SF improves the sensitivity of magnetic resonance of water molecules through dynamic nuclear polarization (DNP).

Anion specific conformational change in aqueous gellan gum solutions.

I NMR is applied to investigate the motional state and the selective interaction of I ions in tetramethylammonium form of gellan gum (TMA gellan), together with monitoring the conformational change by the optical rotation measurement. The experimental results indicate that I ion promotes the conformational transition, and there exists the preferential affinity of I ion for the ordered conformation of TMA gellan.

Theoretical study on the mechanism of the hydrogen evolution reaction catalyzed by platinum subnanoclusters.

The smallest subnanocluster models of platinum colloid (Pt) are supposed to diffuse in aqueous media in order to examine their behaviors when they are subjected to the electrocatalytic hydrogen evolution reaction under zero overpotential conditions at pH 0. The DFT approach allows us to clarify the nature of individual proton transfer (PT) and electron transfer (ET) processes together with the importance of relying on concerted proton-electron transfer (CPET) pathways to promote the majority of H* adsorption processes by Pt subnanoclusters. Although the CPET processes are closely correlated with the Volmer steps (Pt + H + e → Pt-H*) described so far in electrochemistry, our study for the first time points out the essential capability of the Pt clusters to promote the multiple PT steps without the need to transfer any electrons, revealing the fundamentally high basicity of the naked Pt clusters (p = 27-28 for Pt, Pt, and Pt).

Impact of sphingomyelin acyl chain heterogeneity upon properties of raft-like membranes.

Sphingomyelin (SM) is a main component of lipid rafts and characteristic of abundance of long and saturated acyl chains. Recently, we reported that fluorescence-labeled lipids including C16:0 and C18:0SMs retained membrane behaviors of inherent lipids. Here, we newly prepared fluorescent SMs with longer acyl chains, C22:0 and C24:1, for observing their partition and diffusion in SM/cholesterol (chol)/dioleoylphosphatidylcholine (DOPC) bilayers.

Generation of transmitochondrial cybrids using a microfluidic device.

Mitochondrial cloning is a promising approach to achieve homoplasmic mitochondrial DNA (mtDNA) mutations. We previously developed a microfluidic device that performs single mitochondrion transfer from a mtDNA-intact cell to a mtDNA-less (ρ) cell by promoting cytoplasmic connection through a microtunnel between them. In the present study, we described a method for generating transmitochondrial cybrids using the microfluidic device.

Duration of fever and other symptoms after the inhalation of laninamivir octanoate hydrate: A study of the 2017/18 and 2018/19 seasons and comparison with the 2011/12 to 2016/17 Japanese influenza seasons.

Introduction: Large scale investigation of the clinical effectiveness of neuraminidase inhibitors for circulating influenza viruses are important along with the surveillance of virus susceptibility in vitro.

Methods: The duration of fever and other influenza symptoms as markers of the clinical effectiveness of laninamivir octanoate hydrate (laninamivir) were investigated in the Japanese 2017/18 and 2018/19 influenza seasons and compared with the results of the previous six seasons.

Results: Influenza A(H1N1)pdm09, A(H3N2), and B were found in 14, 45, and 52 patients in the 2017/18 season and in 22, 62, and 0 in the 2018/19 season, respectively.