2 results match your criteria: "Kyono ART Clinic Mitsuiseimei[Affiliation]"
Comparison of Residual Dimethyl Sulfoxide (DMSO) and Ethylene Glycol (EG) Concentration in Bovine Ovarian Tissue During Warming Steps Between Slow Freezing and Vitrification.
Cryo Letters
May 2019
Kyono ART Clinic Mitsuiseimei, Sendai Honcho Bl, 3F 1-1-1 Honcho, Aoba-ku, Sendai, Miyagi, 980-0014, Japan. Kyono ART Clinic Takanawa, Takanawa 3-13-1 Takanawa Court 5F, Minato-ku, Tokyo, 108-0074, Japan.
Background: DMSO and EG have been used as cryoprotectants for human ovarian tissue cryopreservation, but residual cryoprotectants concentration and safety have rarely been reported.
Objective: We aimed to compare residual cryoprotectants (DMSO, EG) concentration in bovine ovarian tissue during warming steps between one kind of common slow freezing method and two kinds of vitrification methods, which are usually used for cryopreservation of human ovarian tissue in Japan.
Materials And Methods: In this study, we used five bovine ovaries with an average age of 24.
Evaluation of oxygen consumption in human vitrified and warmed pre-antral follicles after prolonged low temperatures.
Reprod Med Biol
January 2014
Kyono ART Clinic Mitsuiseimei, Sendai Honcho Bldg, 3F, 1-1-1 Honcho, Aoba-ku 980-0014 Sendai Miyagi Japan.
Purpose: The purpose of this study was to evaluate the effect of transportation at prolonged low temperatures on the survival of pre-antral follicles.
Methods: Ovarian tissue was removed from six women with gender identity disorder. Tissues were stored in an icebox at 4 °C for 6 or 18 h prior to vitrification.