Laser-assisted hatching on clinical and neonatal outcomes in patients undergoing single vitrified Blastocyst transfer: A propensity score-matched study.

Purpose: This study determined the effect of laser-assisted hatching on the clinical and neonatal outcomes of single vitrified blastocyst transfer.

Methods: From June 2014 to March 2018, 289 matched pairs after propensity score matching were analyzed. During the blastocyst warming procedure, a small section of the zona pellucida area in the empty perivitelline space was sliced off using multiple laser beams.

Effect of equilibration time on clinical and neonatal outcomes in human blastocysts vitrification.

Purpose: Prolonged exposure to equilibration solutions may be detrimental to an embryo's developmental potential, whereas a shorter exposure may affect the penetration of cryoprotectants into blastomeres. The purpose of this study was to evaluate the effects of different equilibration times on the clinical and neonatal outcomes of human blastocyst vitrification.

Methods: This is a retrospective study based on data collected between November 2008 and November 2015.

Evaluation of the effect of piezo-intracytoplasmic sperm injection on the laboratory, clinical, and neonatal outcomes.

Purpose: Limited research has been published on the effect of piezo-assisted intracytoplasmic sperm injection (P-ICSI). We evaluated the effect of P-ICSI on the laboratory, clinical, and neonatal outcomes.

Methods: This retrospective study was based on the data collected between April 2011 and October 2016.

Effect on clinical and neonatal outcomes of blastocelic microsuction prior to vitrification.

Purpose: Microsuction (MS) is a technique for mechanically emptying fluid from blastocele using a microneedle. In this study, we evaluated the improvement in clinical and neonatal outcomes of vitrified blastocyst transfer programs when MS of blastocelic fluid was used before vitrification.

Methods: This was a retrospective study based on data collected between March 2014 and August 2016.

Successful delivery derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia.

Objective: To report the clinical outcomes following intracytoplasmic sperm injection (ICSI) with vitrified sperm from patients with severe male factor infertility.

Design: Retrospective case series.

Setting: IVF unit of a medical center.

Simple vitrification for small numbers of human spermatozoa.

Conventional freezing procedures and containers are not appropriate for spermatozoa from the testis because of their low number and poor in-situ motility, and various types of container have been utilized to freeze small numbers of spermatozoa. This study tried to develop a vitrification method for small numbers of spermatozoa using the Cell Sleeper, which is a closed type of cell-cryopreservation container. The container with spermatozoa were cooled in liquid nitrogen vapour and then stored in a cryotank.