Characterization of a maltononaose-producing amylopullulanase from Bacillus aryabhattai W310.

The recombinated amylopullulanase of PulW310B, pullulanase from Bacillus aryabhattai W310, was characterized. Sequence analysis of PulW310B showed that PulW310B has type I pullulanase structures including its typical region and the conserved regions of glycoside hydrolase family 13. Moreover, PulW310B was predicted to has typical domains of pullulanase and SSF51445 belonging to tansglycosidase.

Regulation of multilayer long crystalline MoS by NiO-modified AlO for improved hydrodesulfurization of COS.

Activated alumina-based composite oxide support is a commonly used support in hydrodesulfurization (HDS) catalysts, which not only retains the performance advantages of each component in the composite oxide support, but also significantly improves the defects of a single activated alumina support, such as the strong metal-support force of interaction. In this study, NiO-AlO composite support was prepared by introducing NiO-modified AlO, and MoS/NiO-AlO catalysts were prepared for the hydrodesulfurization of COS by liquid phase reduction method. In the COS hydrogenation reaction, the MoS/NiO-AlO catalyst achieved complete conversion of COS at 220 °C and showed more than 99.

Zscan4 mediates ubiquitination and degradation of the corepressor complex to promote chromatin accessibility in 2C-like cells.

Zygotic genome activation occurs in two-cell (2C) embryos, and a 2C-like state is also activated in sporadic (~1%) naïve embryonic stem cells in mice. Elevated chromatin accessibility is critical for the 2C-like state to occur, yet the underlying molecular mechanisms remain elusive. Zscan4 exhibits burst expression in 2C embryos and 2C-like cells.

Predictive model for HBsAg clearance rate in chronic hepatitis B patients treated with pegylated interferon α-2b for 48 weeks.

Background And Aims: Chronic hepatitis B (CHB) is a major global health concern. This study aims to investigate the factors influencing hepatitis B surface antigen (HBsAg) clearance in CHB patients treated with pegylated interferon α-2b (Peg-IFNα-2b) for 48 weeks and to establish a predictive model.

Methods: This analysis is based on the "OASIS" project, a prospective real-world multicenter study in China.

Identification of a novel peptide with anti-inflammatory activity from Binglangjiang buffalo fermented milk and its potential inhibitory mechanism in lipopolysaccharide-stimulated RAW264.7 cells.

Binglangjiang buffalo fermented milk (BBFM) is rich in bioactive peptides and exhibits significant immunomodulatory activity, although the specific components and effects are not well understood. In this study, we investigated the anti-inflammatory activity peptides from BBFM using peptidomics and proteomics. A total of 769 peptides were identified using LC-MS/MS.

Comparative effectiveness of non-pharmacological therapies for postoperative cognitive dysfunction: Protocol for a systematic review and network meta-analysis.

Introduction: Postoperative cognitive dysfunction (POCD) is a common complication following surgery. Electroacupuncture (EA), manual acupuncture (MA), transcutaneous electrical acupoint stimulation (TEAS), and cognitive training (CT) can effectively maintain or improve the postoperative cognitive function of patients. However, it remains unclear which therapy is the most effective.

Single-point ultrasound-guided iliohypogastric-ilioinguinal-genitofemoral nerve block for inguinal hernia surgery in older adult patients: a randomized controlled trial.

Background: Ultrasound-guided nerve block can be used for perioperative analgesia and can potentially improve the course of recovery. Although iliohypogastric-ilioinguinal nerve block has been successfully used for inguinal hernia surgery, the poor blocking effect of intraoperative traction reflex remains a major drawback of this technique. The main objective of this study was to investigate the feasibility of single-point ultrasound-guided iliohypogastric-ilioinguinal-genitofemoral nerve (GFN) blockage for open anterior inguinal hernia repair in older adults.

Comparative analysis of antioxidant activity and structural changes of polysaccharides between sulfur-fumigation and nonsulfur-fumigation.

Introduction: (referred to Tianma in Chinese), the dried tuber of Bl. (Orchidaceae), is utilized as a medicine-food homology product. Sulfur fumigation is commonly employed in the processing of (GR).

Predicting Precursor Ions Combined with Fragmentation Pathway for Screening and Identification of Flavan-3-ol Oligomers in Tea (. var. ).

Flavan-3-ol oligomers (FLOs), including proanthocyanidins (PAs) and theasinensins (TSs), contribute greatly to the flavor and bioactivity of the tea beverage. Ultrahigh-performance liquid chromatography coupled with high-resolution mass spectrometry has been widely used in profiling a wide range of compounds in tea. However, the detection and identification of FLOs with low concentration and high structural diversity remain meaningful yet challenging work.

[Biomechanical study of knee joint based on coronal plane alignment of the knee].

Objective: To establish a finite element model of the knee joint based on coronal plane alignment of the knee (CPAK) typing method, and analyze the biomechanical characteristics of different types of knee joints.

Methods: The finite element models of the knee joint were established based on CT scan data of 6 healthy volunteers. There were 5 males and 1 female with an average age of 24.

Windfarm construction alters soil multinutrient cycling by destabilizing microfauna community in a mountain ecosystem.

In recent decades, investors attracted to wind power's promise of zero-emission electricity have fueled the proliferation of large windfarms across the world. However, the effects of windfarm construction with different land use subtypes (i.e.

Remodel Heterogeneous Electrical Microenvironment at Nano-Scale Interface Optimizes Osteogenesis by Coupling of Immunomodulation and Angiogenesis.

Immunomodulation is essential for implants to regulate tissue regeneration, while bioelectricity plays a fundamental role in regulating immune activities. Under natural preferences, the bone matrix electrical microenvironment is heterogeneous in the nanoscale, which provides fundamental electrical cues to regulate bone immunity and regenerative repair. However, remodeling bone nanoscale heterogeneous electrical microenvironment remains a challenge, and the underlying immune modulation mechanism remains to be explored.

Scaling behavior of cross-entropy loss in the identification of percolation phase transitions.

The cross-entropy loss function is widely used in machine learning to measure the performance of a classification model. Interestingly, our study find that this function has scaling behavior when deep neural networks are used to investigate percolation models. Specifically, we use convolutional neural networks with different pooling methods to study the site percolation on square lattices under two labeling methods (labeling based on spanning cluster and the exact solution of the critical point).

Reductive Dissolution Mechanisms of Manganese Oxide Mediated by Algal Extracellular Organic Matter and the Effects on 17α-Ethinylestradiol Removal.

Reductive dissolution of manganese oxide (MnO) is a major process that improves the availability of manganese in natural aquatic environments. The extracellular organic matter (EOM) secreted by algae omnipresent in eutrophic waters may affect MnO dissolution thus the fate of organic micropollutants. This study investigates the mechanisms of MnO reductive dissolution mediated by EOM and examines the effects of this process on 17α-ethinylestradiol degradation.

Copper-Cobalt Nanozyme Mimicking Laccase for Sensitive Colorimetric Determination of Cannabidiol in Food and Cosmetics.

In colorimetric analysis, nanozymes are invaluable tools due to their simple production, long-lasting stability, and adaptable enzymatic activity, which enable them to induce changes in substrate color. In this study, a simple nanozyme-based colorimetric sensor was developed to detect cannabidiol (CBD) by using the laccase activity of the self-made MOF with copper and cobalt loading (Cu/Co@MOF) nanozyme, which was synthesized using a one-pot microwave method. The Cu/Co@MOF has the ability to catalyze the coupling reaction between 4-AP and various phenolic substrates, thereby converting colorless phenolic substrates into red substances.

Strong self-association of chitosan microgels at interface mediated high stabilities in Pickering emulsion.

The spontaneous self-organization of naturally-occurring polysaccharide particles into a thick and robust gel network at interface in Pickering emulsion is challenging. Inspired by the phenomenon that chitosan microgels (CSMs) with a certain size could self-associate into a solidified gel phase upon freezing, here we tentatively used CSMs to construct a highly-stable Pickering emulsion. CSMs can form a stable Langmuir's layer at the water/oil interface through the network deformation and re-arrangement of dangling chains, while the subsequent negative polymer coating can avoid the bridging resulting from the cross-association for CSMs on different emulsion droplets upon freezing.

Effects of folate biosynthesis defects in Lactiplantibacillus plantarum.

Folate is an essential nutrient for nearly all organisms. While the physiological function and mechanism aspects of folate have been extensively and deeply investigated in Eukarya, related researches in Bacteria remains poorly understood. In this study, we focus on physiological functions of folate in Lactiplantibacillus plantarum by employing a combination of genetics, biochemistry and microscopy approaches.

synthesis of an Al and V co-doped TiO NTA interlayer-enhanced PbO composite for efficient zinc electrowinning.

The high overpotential of the oxygen evolution reaction (OER) and the strong corrosion of the anode are the main problems currently faced by the zinc hydrometallurgical process. This study achieved the successful synthesis of titanium dioxide nanotubes doped by Al and V on a TC4 alloy. Subsequently, a composite electrode, TC4/AVTN-7/PbO-ZrO-CoO, was prepared utilizing composite electrodeposition.

Toward synergetic reduction of pollutant and greenhouse gas emissions from vehicles: a catalysis perspective.

It is a great challenge for vehicles to satisfy the increasingly stringent emission regulations for pollutants and greenhouse gases. Throughout the history of the development of vehicle emission control technology, catalysts have always been in the core position of vehicle aftertreatment. Aiming to address the significant demand for synergistic control of pollutants and greenhouse gases from vehicles, this review provides a panoramic view of emission control technologies and key aftertreatment catalysts for vehicles using fossil fuels (gasoline, diesel, and natural gas) and carbon-neutral fuels (hydrogen, ammonia, and green alcohols).

UV/Ozone-Induced Interface Engineering for High-Performance Horizontal Organic Light-Emitting Transistors Operating at Low Voltage.

Multifunctional organic light-emitting transistors (OLETs), which combine electric-switching and light-producing capabilities into a single device, are attracting increasing interest as promising candidates for new-generation display technology. Despite advancements in the design of organic luminescent materials and the optimization of device geometry configurations, maintaining operating voltage low while enhancing optical performances remains a key challenge in horizontally structured OLETs. Here, a simple and effective interfacial engineering strategy is employed to improve the optical properties of horizontal OLETs operating at low voltage, by introducing ultraviolet ozone (UVO)-induced surface modification on high-k dielectrics.

A Temporal Network Based on Characterizing and Extracting Time Series in Copper Smelting for Predicting Matte Grade.

Addressing the issues of low prediction accuracy and poor interpretability in traditional matte grade prediction models, which rely on pre-smelting input and assay data for regression, we incorporate process sensors' data and propose a temporal network based on Time to Vector (Time2Vec) and temporal convolutional network combined with temporal multi-head attention (TCN-TMHA) to tackle the weak temporal characteristics and uncertain periodic information in the copper smelting process. Firstly, we employed the maximum information coefficient (MIC) criterion to select temporal process sensors' data strongly correlated with matte grade. Secondly, we used a Time2Vec module to extract periodic information from the copper smelting process variables, incorporates time series processing directly into the prediction model.

LM-CycleGAN: Improving Underwater Image Quality Through Learned Perceptual Image Patch Similarity and Multi-Scale Adaptive Fusion Attention.

The underwater imaging process is often hindered by high noise levels, blurring, and color distortion due to light scattering, absorption, and suspended particles in the water. To address the challenges of image enhancement in complex underwater environments, this paper proposes an underwater image color correction and detail enhancement model based on an improved Cycle-consistent Generative Adversarial Network (CycleGAN), named LPIPS-MAFA CycleGAN (LM-CycleGAN). The model integrates a Multi-scale Adaptive Fusion Attention (MAFA) mechanism into the generator architecture to enhance its ability to perceive image details.

The Effect of Cyclic Heat Treatment on the Microstructure and Mechanical Properties of 18CrNiMo7-6 Gear Steel.

To avoid grain coarsening resulting from high-temperature carburizing, the effects of cyclic quenching and tempering on the microstructure and mechanical properties of 18CrNiMo7-6 gear steel were investigated. Three groups of samples were compared, which went through 0/1/3 times of quenching-tempering cycles after initial pseudo-carburizing. The variations in grain size, hardness, tensile strength, and toughness were systematically assessed using a series of experimental techniques.

Hydrogen Embrittlement Sensitivity of X70 Welded Pipe Under a High-Pressure Pure Hydrogen Environment.

With the rapid development of hydrogen pipelines, their safety issues have become increasingly prominent. In order to evaluate the properties of pipeline materials under a high-pressure hydrogen environment, this study investigates the hydrogen embrittlement sensitivity of X70 welded pipe in a 10 MPa high-pressure hydrogen environment, using slow strain rate testing (SSRT) and low-cycle fatigue (LCF) analysis. The microstructure, slow tensile and fatigue fracture morphology of base metal (BM) and weld metal (WM) were characterized and analyzed by means of ultra-depth microscope, scanning electron microscope (SEM), electron backscattering diffraction (EBSD), and transmission electron microscope (TEM).