Single-cell analysis reveals the loss of FABP4-positive proliferating valvular endothelial cells relates to functional mitral regurgitation.

Background: Functional mitral regurgitation (MR) is a common form of mitral valve dysfunction that often persists even after surgical intervention, requiring reoperation in some cases. To advance our understanding of the pathogenesis of functional MR, it is crucial to characterize the cellular composition of the mitral valve leaflet and identify molecular changes in each cell subtype within the mitral valves of MR patients. Therefore, we aimed to comprehensively examine the cellular and molecular components of mitral valves in patients with MR.

Effects of different temperatures on chondrocyte growth: a transcriptomic analysis.

Background: Our previous study demonstrated that temperature-related microwave ablation (MWA) can safely modulate growth plates of piglets' vertebrae. Therefore, this study is designed to investigate the effects of different temperatures on chondrocyte viability and the underlying molecular mechanisms in vitro.

Methods: Following a 10-minute treatment at different temperatures (37 °C, 40 °C, 42 °C, 44 °C, 46 °C, 48 °C, and 50 °C), CCK-8 assay was used to examine the viability of ATDC5 cells at 12 h.

Anti-neuroinflammatory terpenes from Magnolia grandiflora.

Six previously undescribed sesquiterpenes, magnogranoides A-F (1-6), along with 10 known terpenes, were isolated from the leaves of Magnolia grandiflora L. Six previously undescribed sesquiterpene derivatives (11a-11e and 14a) were synthesized using chemical methods. Those structures were identified through extensive spectroscopic data and quantum chemical calculations.

Unveiling the hepatoprotective mechanisms of Desmodium heterocarpon (L.) DC: Novel flavonoid identification and Keap1/Nrf2 pathway activation.

Background: The pathophysiology of liver diseases is significantly influenced by oxidative stress, making its alleviation a key strategy for treatment. The Keap1/Nrf2 signaling pathway is the body's most crucial antioxidant defense mechanism. Traditional Chinese medicine, Desmodium heterocarpon (L.

Effects of mixed exposure to PFAS on adolescent non-alcoholic fatty liver disease: Integrating evidence from human cohorts, toxicogenomics, and animal models to uncover mechanisms and potential target sites.

Extensive evidence suggests a correlation between environmental pollutants, specifically perfluoroalkyl and polyfluoroalkyl substances (PFAS) and non-alcoholic fatty liver disease (NAFLD). This study aims to investigate the association and underlying mechanisms of PFAS-induced NAFLD in adolescents by employing a comprehensive approach of population-based studies, toxicogenomics, and animal models. A total of 2014 freshmen from Dali University were recruited for this study, with 1694 participants undergoing serum testing for PFAS exposure.

Declining trends and regional variations of organophosphate ester contamination in indoor dust from mainland China: Insights from a filed study and meta-analysis.

This study investigated the presence of 20 organophosphate esters (OPEs) in indoor dust samples collected from the Chinese cities of Lanzhou, Xining, and Lhasa. The results demonstrate the ubiquitous presence of most OPEs in these three cities, with the highest concentrations of ΣOPEs found in Xining. We also summarized the occurrence of OPEs in indoor environments from 38 studies with 1875 samples collected across various regions of mainland China from 2012 to 2023.

Zscan4 mediates ubiquitination and degradation of the corepressor complex to promote chromatin accessibility in 2C-like cells.

Zygotic genome activation occurs in two-cell (2C) embryos, and a 2C-like state is also activated in sporadic (~1%) naïve embryonic stem cells in mice. Elevated chromatin accessibility is critical for the 2C-like state to occur, yet the underlying molecular mechanisms remain elusive. Zscan4 exhibits burst expression in 2C embryos and 2C-like cells.

Protective effect of oleuropein on the brain tissue in D-Galactose-induced aging in rat model.

Background: Oleuropein (OLE) has the potential to reduce oxidative stress and inflammation. So, in the present investigation, we explored the protective effect of OLE on brain aging induced by d-galactose (D-Gal) in a rat model.

Methods And Results: 40 Wister male adult rats were categorized into 5 groups.

DNMT1 inhibition improves the activity of memory-like natural killer cells by enhancing the level of autophagy.

Background: Acute myeloid leukemia (AML) is a common hematological tumor, but it is difficult to treat. DNMT1 is a DNA methyltransferase whose main function is to maintain stable DNA methylation during the DNA replication process. DNMT1 also plays an important role in AML, but its function in cytokine-induced memory-like natural killer (CIML NK) cell activity remains unclear.

Xuanhong Dingchuan Tang suppresses bronchial asthma inflammation via the microRNA-107-3p/PTGS2/MAPK axis.

This study aimed to investigate the mechanism of Xuanhong Dingchuan Tang (XHDCT) in delaying bronchial asthma inflammation via the microRNA (miR)-107-3p/prostaglandin endoperoxide synthase 2 (PTGS2)/mitogen-activated protein kinase (MAPK) axis. Based on the network pharmacological analysis, XHDCT chemical constituents and targets of each chemical constituent were screened through the TCMSP database, and differential-expressed genes of bronchial asthma were obtained from the GEO database, which were intersected to get XHDCT potential anti-inflammatory targets. The key anti-inflammatory targets of XHDCT were acquired by protein-protein interaction (PPI) analysis of the candidate targets.

NEDD4 enhances bone‑tendon healing in rotator cuff tears by reducing fatty infiltration.

Rotator cuff tears (RCT) can cause shoulder pain, weakness and stiffness, significantly affecting daily life. Analysis of the GSE103266 dataset revealed significant changes in the mTOR/PI3K/Akt signaling pathway and lipid metabolism‑related pathways, suggesting that fatty infiltration may affect RCT. The analysis indicated that the ubiquitin ligase NEDD4 plays a critical role in RCT.

A review on functional lung avoidance radiotherapy plan for lung cancer.

Lung cancer is the most common malignant tumor in China. Its incidence and mortality rate increase year by year. In the synthesis treatment of lung cancer, radiotherapy (RT) plays a vital role, and radiation-induced lung injury(RILI) has become the major limiting factor in prescription dose escalation.

Predictive model for HBsAg clearance rate in chronic hepatitis B patients treated with pegylated interferon α-2b for 48 weeks.

Background And Aims: Chronic hepatitis B (CHB) is a major global health concern. This study aims to investigate the factors influencing hepatitis B surface antigen (HBsAg) clearance in CHB patients treated with pegylated interferon α-2b (Peg-IFNα-2b) for 48 weeks and to establish a predictive model.

Methods: This analysis is based on the "OASIS" project, a prospective real-world multicenter study in China.

[Metabolomics study on anti-radiation effect of Yiguan Decoction].

The gas chromatography-mass spectrometer(GC-MS) metabolomics method was used to investigate the anti-radiation effect of Yiguan Decoction water extract on mice and explore its related pathways and mechanisms of action. Healthy SPF male mice from Kunming were randomly assigned to six groups: blank group, model group, positive drug group, and high-dose, medium-dose, and low-dose groups of Yiguan Decoction, with ten mice in each group. Mice were prophylactically dosed for six days, and ~(60)Co γ ray with 5 Gy was used to establish a radiation mouse model.

Taraxasterol extracted from Ixeridium gramineum (Fisch.) Tzvel. Attenuated D-GalN/LPS-induced fulminant hepatitis by modulating the JAK/STAT and TNF signalling pathways.

Ethnopharmacological Relevance: Taraxasterol (TAR), a compound highly abundant and easily obtainable from Tibetan medicine Ixeridium gramineum (Fisch.) Tzvel., exhibits a variety of biological effects, including hepatoprotective, anti-inflammatory, and antioxidant activities.

Application of clown care in hospitalized children: A scoping review.

Purpose: This study aims to provide healthcare providers with insights into relevant interventions by examining the timing, format, content, outcome measures, and effects of clown care interventions on hospitalized children.

Methods: This study utilized a scoping review methodology based on the framework by Arksey and O'Malley. The search for Chinese and English literature on the utilization of clown care for hospitalized children was conducted in databases including CNKI, VIP, Wanfang, China Biology Medicine Database, PubMed, Embase, Web of Science, CINAHL, and Cochrane Library.

Y01 reduces HFD-induced obesity altering gut microbiota and metabolomic profiles and modulating adipose tissue macrophage M1/M2 polarization.

Obesity-related metabolic syndrome is intimately associated with infiltrated adipose tissue macrophages (ATMs), gut microbiota, and metabolic disorders. holds the potential to mitigate obesity; however, there exist strain-specific functionalities and diverse mechanisms, which deserve extensive exploration. This study aims to explore the potential of Y01, isolated from traditional sour whey, in alleviating HFD-induced metabolic syndrome in mice and elucidating its underlying mechanism.

Comparative analysis of the human microbiome from four different regions of China and machine learning-based geographical inference.

The human microbiome, the community of microorganisms that reside on and inside the human body, is critically important for health and disease. However, it is influenced by various factors and may vary among individuals residing in distinct geographic regions. In this study, 220 samples, consisting of sterile swabs from palmar skin and oral and nasal cavities were collected from Chinese Han individuals living in Shanghai, Chifeng, Kunming, and Urumqi, representing the geographic regions of east, northeast, southwest, and northwest China.

An Index-Contact Paired Data Analysis on Sexual Contact Tracing Outcomes of HIV-Infected Individuals - Yunnan Province, China, 2022-2024.

What Is Already Known About This Topic?: A substantial proportion of people living with human immunodeficiency virus (PLWH) remain unaware of their infection status. Contact tracing serves as an effective public health tool for identifying human immunodeficiency virus (HIV) infections and supports progress toward achieving the 95-95-95-95 goals.

What Is Added By This Report?: An egocentric contact tracing study conducted in Yunnan, China, between January 2022 and June 2024 enrolled 1,981 index cases, of whom 314 (15.

Zhuanggu Shubi ointment mediated the characteristic bacteria-intestinal mucosal barrier-bone metabolism axis to intervene in postmenopausal osteoporosis.

Background: Zhuanggu Shubi ointment (ZGSBG) has good efficacy in postmenopausal osteoporosis (PMO), but the mechanism of efficacy involving gut microecology has not been elucidated.

Objective: This study investigated the mechanism of ZGSBG in regulating gut microecology in PMO.

Methods: The bilateral ovarian denervation method was used to construct a rat model of PMO and was administered ZGSBG.